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master's thesis
Utjecaj utišavanja i inhibicije Hsp90 na ekspresiju endotelina-1 u THP-1 stanicama
Zagreb: University of Zagreb, Faculty of Pharmacy and Biochemistry, 2015. urn:nbn:hr:163:938981
Brzić, Kristina
University of Zagreb
Faculty of Pharmacy and Biochemistry
Department of medical biochemistry and haematology

Institutional repository: Repository of Faculty of Pharmacy and Biochemistry University of Zagreb

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Brzić, K. (2015). Utjecaj utišavanja i inhibicije Hsp90 na ekspresiju endotelina-1 u THP-1 stanicama (Master's thesis). Zagreb: University of Zagreb, Faculty of Pharmacy and Biochemistry. Retrieved from https://urn.nsk.hr/urn:nbn:hr:163:938981

Brzić, Kristina. "Utjecaj utišavanja i inhibicije Hsp90 na ekspresiju endotelina-1 u THP-1 stanicama." Master's thesis, University of Zagreb, Faculty of Pharmacy and Biochemistry, 2015. https://urn.nsk.hr/urn:nbn:hr:163:938981

Brzić, Kristina. "Utjecaj utišavanja i inhibicije Hsp90 na ekspresiju endotelina-1 u THP-1 stanicama." Master's thesis, University of Zagreb, Faculty of Pharmacy and Biochemistry, 2015. https://urn.nsk.hr/urn:nbn:hr:163:938981

Brzić, K. (2015). 'Utjecaj utišavanja i inhibicije Hsp90 na ekspresiju endotelina-1 u THP-1 stanicama', Master's thesis, University of Zagreb, Faculty of Pharmacy and Biochemistry, accessed 29 March 2025, https://urn.nsk.hr/urn:nbn:hr:163:938981

Brzić K. Utjecaj utišavanja i inhibicije Hsp90 na ekspresiju endotelina-1 u THP-1 stanicama [Master's thesis]. Zagreb: University of Zagreb, Faculty of Pharmacy and Biochemistry; 2015 [cited 2025 March 29] Available at: https://urn.nsk.hr/urn:nbn:hr:163:938981

K. Brzić, "Utjecaj utišavanja i inhibicije Hsp90 na ekspresiju endotelina-1 u THP-1 stanicama", Master's thesis, University of Zagreb, Faculty of Pharmacy and Biochemistry, Zagreb, 2015. Available at: https://urn.nsk.hr/urn:nbn:hr:163:938981

Metadata
TitleUtjecaj utišavanja i inhibicije Hsp90 na ekspresiju endotelina-1 u THP-1 stanicama
Title (english)Effects of Hsp90 silencing and inhibition on endothelin-1 expression in THP-1 cells
AuthorKristina Brzić
MentorKarmela Barišić (mentor)
MentorJozsef Petrik (mentor)
Committee memberKarmela Barišić
Committee memberJozsef Petrik
Committee memberSandra Šupraha Goreta
GranterUniversity of Zagreb
Faculty of Pharmacy and Biochemistry
(Department of medical biochemistry and haematology)
Zagreb
Defense date and country2015-07-06, Croatia
Scientific / art field,
discipline and subdiscipline		BIOMEDICINE AND HEALTHCARE
Pharmacy
Medical Biochemistry
Abstract
Tumor je patološka promjena koja nastaje kada zdrave stanice organizma započnu nekontroliran rast i razvoj zbog nemogućnosti regulacijskih mehanizama da ih kontroliraju. Jedna od važnih karakteristika tumorskih stanica jest i njihova sposobnost odolijevanja apoptozi tj. programiranoj staničnoj smrti. Hsp90, protein toplinskoga stresa iz obitelji HSP90, konstututivno je eksprimiran u svim stanicama. U stresnim stanjima njegova ekspresija je inducirana. Hsp90 ima različite funkcije, a jedna od... More njegovih funkcija je i inhibicija programirane stanične smrti stabilizacijom čimbenika uključenih u staničnu proliferaciju. Endotelin-1 (ET-1) jest peptid uključen u regulaciju velikog broja fizioloških i patoloških procesa, uključujući i malignu proliferaciju. U brojnim tumorima utvrđena je povećana ekspresija peptida endotelinskoga sustava. Konačan rezultat te prekomjerne ekspresije je aktivacija proliferacije, rezistencija tumorskih stanica na apoptozu, stimulacija nastanka novih krvnih žila, modulacija imunoga odgovora, abnormalna osteogeneza. Svi ovi procesi pridonose invazivnosti i metastaziranju tumora. Glavni cilj ovoga diplomskoga rada bio je procijeniti učinak smanjene prisutnosti (utišavanja) ili inhibicije Hsp90 u tumorskim stanicama na ekspresiju ET-1, vrlo važnoga antiapoptotskoga čimbenika. THP-1 stanice ljudske monocitne leukemije korištene su kao eksperimentalni model tumorskih stanica. Hsp90 je inhibiran na dva načina: 1. RNA interferencijom uz pomoć specifične siRNA (engl. small interfering RNA, siRNA) i 2. tretiranjem stanica kemijskim inhibotorom ekspresije Hsp90, 17-DMAG-om. U mediju iznad THP-1 stanica tretiranih inhibitorima Hsp90 izmjerena je koncentracija ET-1. Kao kontrolni uzorci korišten je medij iznad netretiranih stanica te medij iznad netretiranih stanica u koji je dodana nespecifična RNA. Dobiveni rezultati ukazuju da utišavanje ili inhibicija Hsp90 ne dovode do promjene u ekspresiji ET-1. Less
Abstract (english)
A tumor is an abnormal growth of tissues which can be caused by the collapse of cell regulatory mechanisms for growth and development. Tumor cells can evade apoptosis, programed cell death which has an important role in the protection of the organism from damaged and potentially dangerous cells. Hsp90 is an intracellular stress protein expressed constitutively, and induced by different type of stress. The important role of Hsp90 is to protect cells from apoptosis by stabilising factors that... More promote cell proliferation. Endothelin-1 (ET-1) is a peptid involved in the regulation of numerous of processes, including cancer proliferation. It has been shown that ET-1 is overexpressed in variety of tumors such as prostatic, ovarian, renal, pulmonary, colorectal, cervical, breast, bladder, endometrial carcinom, brain tumors, melanoma and bone metastasis. ET-1 participates in growth, progression, apoptosis, formation of new vesels, invasion, metastasis, abnormal osteogenesis and alternation of nociceptive stimuli. The goal of this study was to examine the role of Hsp90 on ET-1 expression in tumor cells. THP-1 cells, human acute monocytic leukemia cell line, were used as experimental model of tumor cells. Hsp90 was inhibited either, by specific siRNA interference or using chemical inhibitor 17-DMAG. ET-1 expression was measured by determing ET-1 concentrations in media of THP-1 cells after their exposure to 17-DMAG or siRNA specific to Hsp90. Results of this study showed that the expression of ET-1 was not affected either by chemical inhibition nor by siRNA interference of Hsp90. Less
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:163:938981
Study programmeTitle: Medical Biochemistry
Study programme type: university
Study level: integrated undergraduate and graduate
Academic / professional title: magistar/magistra medicinske biokemije (magistar/magistra medicinske biokemije)
Type of resourceText
File originBorn digital
Access conditionsAccess restricted to students and staff of home institution
Terms of useLicence In copyright icon
Created on2016-01-19 15:46:37
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