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master's thesis
Inhibicija virulencije vrste Candida albicans oleuropeinom i hidroksitirosolom
Zagreb: University of Zagreb, Faculty of Pharmacy and Biochemistry, 2017. urn:nbn:hr:163:531956
Bobnjarić, Ivan
University of Zagreb
Faculty of Pharmacy and Biochemistry
Department of microbiology

Institutional repository: Repository of Faculty of Pharmacy and Biochemistry University of Zagreb

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Bobnjarić, I. (2017). Inhibicija virulencije vrste Candida albicans oleuropeinom i hidroksitirosolom (Master's thesis). Zagreb: University of Zagreb, Faculty of Pharmacy and Biochemistry. Retrieved from https://urn.nsk.hr/urn:nbn:hr:163:531956

Bobnjarić, Ivan. "Inhibicija virulencije vrste Candida albicans oleuropeinom i hidroksitirosolom." Master's thesis, University of Zagreb, Faculty of Pharmacy and Biochemistry, 2017. https://urn.nsk.hr/urn:nbn:hr:163:531956

Bobnjarić, Ivan. "Inhibicija virulencije vrste Candida albicans oleuropeinom i hidroksitirosolom." Master's thesis, University of Zagreb, Faculty of Pharmacy and Biochemistry, 2017. https://urn.nsk.hr/urn:nbn:hr:163:531956

Bobnjarić, I. (2017). 'Inhibicija virulencije vrste Candida albicans oleuropeinom i hidroksitirosolom', Master's thesis, University of Zagreb, Faculty of Pharmacy and Biochemistry, accessed 24 December 2024, https://urn.nsk.hr/urn:nbn:hr:163:531956

Bobnjarić I. Inhibicija virulencije vrste Candida albicans oleuropeinom i hidroksitirosolom [Master's thesis]. Zagreb: University of Zagreb, Faculty of Pharmacy and Biochemistry; 2017 [cited 2024 December 24] Available at: https://urn.nsk.hr/urn:nbn:hr:163:531956

I. Bobnjarić, "Inhibicija virulencije vrste Candida albicans oleuropeinom i hidroksitirosolom", Master's thesis, University of Zagreb, Faculty of Pharmacy and Biochemistry, Zagreb, 2017. Available at: https://urn.nsk.hr/urn:nbn:hr:163:531956

Metadata
TitleInhibicija virulencije vrste Candida albicans oleuropeinom i hidroksitirosolom
Title (english)Inhibition of Candida albicans virulation factors with oleuropein and hydroxytyrosol
AuthorIvan Bobnjarić
MentorIvan Kosalec (mentor)
Committee memberIvan Kosalec (predsjednik povjerenstva)
Committee memberDavor Šakić (član povjerenstva)
GranterUniversity of Zagreb
Faculty of Pharmacy and Biochemistry
(Department of microbiology)
Zagreb
Defense date and country2017-09-28, Croatia
Scientific / art field,
discipline and subdiscipline		BIOMEDICINE AND HEALTHCARE
Pharmacy
Pharmacy
Abstract
Candida albicans je dio ljudskog mikrobioma (endogeni oportunistički kvasac koji u zdravih osoba ne uzrokuje infekciju). No, narušavanjem ravnoteže između mikrobioma i nositelja, komenzalni oblik prelazi u infekciju s kliničkom manifestacijom. Cilj ovoga rada je utvrditi učinak oleuropeina i hidroksitirosola te njihov menhanizam djelovanja na vrstu Candida albicans. Određivanjem ergosterola SQM metodom utvrđeno je da oleuropein i hidroksitirosol u sub-MIK vrijednostima nakon 18 sati inkubacije na 35°C daju rezultate koji pokazuju njegovo uplitanje u put biosinteze ergosterola. Određivanjem učinka hidroksitirosola i oleuropeina na inhibiciju germinacije blastospora vrste C. albicans pokazalo se oleuropein i hidroksitirosol nakon 3 sata inkubacije na 37 °C inhibiraju germinaciju blastospora već u sub-MIK koncentracijama djelovanjem na unutarstanične signalne puteve (cAMP-PKA). Ispitivanjem učinka na rast filamenata otkriveno je da oleuropein i hidroksitirosol nakon 3 sata inkubacije na 37 °C inhibiraju rast filamenata germiniranih stanica vrste C. albicans već u sub-MIK koncentracijama. Iz dobivenih se podataka može zaključiti da su oleuropein i hidroksitirosol izrazito zanimljive molekule za daljnja istraživanja mehanizama antifungalnog učinka, ali i mogućeg sinergističkog učinka s antimikoticima. Prema tome, potrebna su daljnja istraživanja mehanizama djelovanja na faktore virulencije.
Abstract (english)
Candida albicans is an endogenous opportunistic yeast that does not cause infections in healthy individuals. However, disruption of the balance between microbiome and microorganism, the commensal form becomes pathogenic with clinical manifestation. The aim ofthis study was to determine the effect of oleuropein and hydroxytyrosol and their mechanism of action in C. albicans in vitro. Using the SQM approach for modulation ofergosterol synthesiswe found that oleuropein and hydroxytyrosol in concentrations below the MIC after 18 hours of incubation at 35 °C showed their interference in ergosterol biosynthesis pathway. The testing of the effect of hydroxytrosol and oleuropein on the inhibition of blastospore germination of C. albicans showed that after 3 hours of incubation at 37 °C oleuropein and hydroxytyrosol inhibit the germination even in values below MIC affecting the cellular signal pathways (cAMP-PKA). The testing of inhibition of growth of filaments reveals that oleuropein and hydroxytyrosol after 3 hours of incubation at 37 °C inhibit the growth of filaments of C. albicans germinated cells even in sub-MIC concentrations. Oleuropein and hydroxytyrosol are extremely interesting molecules for further research into mechanisms of antifungal activity, but also for possible synergistic effect with antimycotics. Therefore, further research into mechanisms of action on virulence factors is required.
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:163:531956
Study programmeTitle: Pharmacy
Study programme type: university
Study level: integrated undergraduate and graduate
Academic / professional title: magistar/magistra farmacije (magistar/magistra farmacije)
Type of resourceText
File originBorn digital
Access conditionsOpen access
Terms of useLicence In copyright icon
Created on2017-11-29 11:27:03