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master's thesis
Utjecaj promjene glikozilacije humanog serumskog transferina na otpuštanje željeza u prisutnosti kompetitivnog kelatora EDTA
Zagreb: University of Zagreb, Faculty of Pharmacy and Biochemistry, 2017. urn:nbn:hr:163:525460
Deriš, Helena
University of Zagreb
Faculty of Pharmacy and Biochemistry
Department of general and inorganic chemistry

Institutional repository: Repository of Faculty of Pharmacy and Biochemistry University of Zagreb

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Deriš, H. (2017). Utjecaj promjene glikozilacije humanog serumskog transferina na otpuštanje željeza u prisutnosti kompetitivnog kelatora EDTA (Master's thesis). Zagreb: University of Zagreb, Faculty of Pharmacy and Biochemistry. Retrieved from https://urn.nsk.hr/urn:nbn:hr:163:525460

Deriš, Helena. "Utjecaj promjene glikozilacije humanog serumskog transferina na otpuštanje željeza u prisutnosti kompetitivnog kelatora EDTA." Master's thesis, University of Zagreb, Faculty of Pharmacy and Biochemistry, 2017. https://urn.nsk.hr/urn:nbn:hr:163:525460

Deriš, Helena. "Utjecaj promjene glikozilacije humanog serumskog transferina na otpuštanje željeza u prisutnosti kompetitivnog kelatora EDTA." Master's thesis, University of Zagreb, Faculty of Pharmacy and Biochemistry, 2017. https://urn.nsk.hr/urn:nbn:hr:163:525460

Deriš, H. (2017). 'Utjecaj promjene glikozilacije humanog serumskog transferina na otpuštanje željeza u prisutnosti kompetitivnog kelatora EDTA', Master's thesis, University of Zagreb, Faculty of Pharmacy and Biochemistry, accessed 25 November 2024, https://urn.nsk.hr/urn:nbn:hr:163:525460

Deriš H. Utjecaj promjene glikozilacije humanog serumskog transferina na otpuštanje željeza u prisutnosti kompetitivnog kelatora EDTA [Master's thesis]. Zagreb: University of Zagreb, Faculty of Pharmacy and Biochemistry; 2017 [cited 2024 November 25] Available at: https://urn.nsk.hr/urn:nbn:hr:163:525460

H. Deriš, "Utjecaj promjene glikozilacije humanog serumskog transferina na otpuštanje željeza u prisutnosti kompetitivnog kelatora EDTA", Master's thesis, University of Zagreb, Faculty of Pharmacy and Biochemistry, Zagreb, 2017. Available at: https://urn.nsk.hr/urn:nbn:hr:163:525460

Metadata
TitleUtjecaj promjene glikozilacije humanog serumskog transferina na otpuštanje željeza u prisutnosti kompetitivnog kelatora EDTA
Title (english)Effect of change in glycosylation of human serum transferrin on iron release to competing chelator EDTA
AuthorHelena Deriš
MentorTin Weitner (mentor)
Committee memberTin Weitner (predsjednik povjerenstva)
Committee memberMario Gabričević (član povjerenstva)
Committee memberDavor Šakić (član povjerenstva)
GranterUniversity of Zagreb
Faculty of Pharmacy and Biochemistry
(Department of general and inorganic chemistry)
Zagreb
Defense date and country2017-11-23, Croatia
Scientific / art field,
discipline and subdiscipline		BIOMEDICINE AND HEALTHCARE
Pharmacy
Pharmacy
Abstract
U ovom radu proučavan je utjecaj promjene glikozilacije transferina na konstantu vezanja željeza. Svrha rada bila je dokazati utjecaj glikanskog dijela strukture transferina na njegovu funkciju, točnije, na jačinu vezanja željeza. Sva ispitivanja su se provodila u PIPES puferu pri pH 7.4, na temperaturi 25°C, a kompleksirajući kompetitivni agens bila je EDTA. Korištena tehnika bila je spektroskopija u UV-Vis području. Iz podataka o apsorbanciji na 450 nm i koncentraciji dodane EDTA, izračunate su najprije pripadajuće koncentracije apo-transferina i holo-transferina te koncentracija otpuštenog željeza, a zatim i odgovarajuće konstante ravnoteže. Dobivene konstante ravnoteže veće su za red veličine kod vezanja željeza za nativni transferin u odnosu na asijalo-transferin. Također, kinetika otpuštanja željeza s asijalo-transferina je promijenjena u odnosu na nativnu sijaliniziranu formu.
Abstract (english)
This work investigates the effect of change of transferrin glycosylation on the iron binding constant. The purpose of this study was to demonstrate the influence of the glycan part of the transferrin structure on its function, specifically on the iron binding strength. All experiments were carried out by UV-Vis spectroscopy in PIPES buffer (pH = 7.4) at 25° C and EDTA was used as a competitive chelator. Based on the absorbance data at 450 nm and the concentration of the added EDTA, concentrations of apo-transferin, holo-transferin and free iron were first obtained and then the corresponding equilibrium constants were calculated. The obtained equilibrium constants are an order of magnitude higher for the iron binding to the native transferrin, compared to asialo-transferrin. Additionally, the iron release kinetics from asialo-transferrin has been altered relative to the native sialylated protein form.
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:163:525460
Study programmeTitle: Pharmacy
Study programme type: university
Study level: integrated undergraduate and graduate
Academic / professional title: magistar/magistra farmacije (magistar/magistra farmacije)
Type of resourceText
File originBorn digital
Access conditionsOpen access
Terms of useLicence In copyright icon
Created on2018-01-24 16:20:29