prikaz prve stranice dokumenta Genotoxicity of fumonisin B1, beauvericin and ochratoxin A in porcine kidney PK15 cells: effects of individual and combined treatment
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Scientific paper - Original scientific paper
Genotoxicity of fumonisin B1, beauvericin and ochratoxin A in porcine kidney PK15 cells: effects of individual and combined treatment
Croatica Chemica Acta, 81 (2008), 1; 139-146. urn:nbn:hr:163:459799
Šegvić Klarić, Maja; Pepeljnjak, Stjepan; Rozgaj, Ružica

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Šegvić Klarić, M., Pepeljnjak, S. & Rozgaj, R. (2008). Genotoxicity of fumonisin B1, beauvericin and ochratoxin A in porcine kidney PK15 cells: effects of individual and combined treatment. Croatica Chemica Acta, 81. (1), 139-146. Retrieved from https://urn.nsk.hr/urn:nbn:hr:163:459799

Šegvić Klarić, Maja, et al. "Genotoxicity of fumonisin B1, beauvericin and ochratoxin A in porcine kidney PK15 cells: effects of individual and combined treatment." Croatica Chemica Acta, vol. 81, no. 1, 2008, pp. 139-146. https://urn.nsk.hr/urn:nbn:hr:163:459799

Šegvić Klarić, Maja, Stjepan Pepeljnjak and Ružica Rozgaj. "Genotoxicity of fumonisin B1, beauvericin and ochratoxin A in porcine kidney PK15 cells: effects of individual and combined treatment." Croatica Chemica Acta 81, no. 1 (2008): 139-146. https://urn.nsk.hr/urn:nbn:hr:163:459799

Šegvić Klarić, M., Pepeljnjak, S. and Rozgaj, R. (2008) 'Genotoxicity of fumonisin B1, beauvericin and ochratoxin A in porcine kidney PK15 cells: effects of individual and combined treatment', Croatica Chemica Acta, 81(1), pp. 139-146. Available at: https://urn.nsk.hr/urn:nbn:hr:163:459799 (Accessed 26 November 2024)

Šegvić Klarić M, Pepeljnjak S, Rozgaj R. Genotoxicity of fumonisin B1, beauvericin and ochratoxin A in porcine kidney PK15 cells: effects of individual and combined treatment. Croatica Chemica Acta [Internet]. 2008 April 15 [cited 2024 November 26];81(1):139-146. Available at: https://urn.nsk.hr/urn:nbn:hr:163:459799

M. Šegvić Klarić, S. Pepeljnjak and R. Rozgaj, "Genotoxicity of fumonisin B1, beauvericin and ochratoxin A in porcine kidney PK15 cells: effects of individual and combined treatment", Croatica Chemica Acta, vol. 81, no. 1, pp. 139-146, April 2008. [Online]. Available at: https://urn.nsk.hr/urn:nbn:hr:163:459799. [Accessed: 26 November 2024]

Metadata
Title (english)Genotoxicity of fumonisin B1, beauvericin and ochratoxin A in porcine kidney PK15 cells: effects of individual and combined treatment
AuthorMaja Šegvić Klarić
AuthorStjepan Pepeljnjak
AuthorRužica Rozgaj
Author's institutionUniversity of Zagreb
Faculty of Pharmacy and Biochemistry
(Department of microbiology)
Scientific / art field,
discipline and subdiscipline		BIOMEDICINE AND HEALTHCARE
Pharmacy
Pharmacy
Abstract (english)
After 24- and 48-hour treatment with 0.05, 0.5 and 5 mg/mL of fumonisin (FB1), beauvericin (BEA) and ochratoxin A (OTA) alone or combinations of two or all three mycotoxins in equal concentrations, Giemsa-stained binucleated PK15 cells were scored for the presence of micronuclei (MN), nuclear buds (NBs) and nucleoplasmic bridges (NPBs). Single mycotoxins induced MN and NPBs in a dose-dependent manner, showing predominantly clastogenic effect. OTA showed stronger genotoxic potential than FB1 and BEA, since 0.05 and 0.5 mg/mL OTA increased the frequency of MN and NPBs, respectively. Mycotoxin combinations increased the frequency of MN and NPBs, mostly in additive manner. A dose-dependent increase in NBs was observed after 24-hour exposure to single mycotoxins. Prolonged treatment with 5 mg/mL of each toxin alone as well as combined exposure to all mycotoxin concentrations resulted in a decrease in NBs, which could be related to the extrusion of micronuclei and/or saturation of the genotoxic effect. This is the first report on genotoxicity of BEA.
Keywords (english)
Languageenglish
Publication typeScientific paper - Original scientific paper
Publication statusPublished
Peer reviewPeer review - international
Publication versionPublished version
Journal titleCroatica Chemica Acta
Numberingvol. 81, no. 1, pp. 139-146
p-ISSN0011-1643
e-ISSN1334-417X
URN:NBNurn:nbn:hr:163:459799
Publication2008-04-15
Document URLhttps://hrcak.srce.hr/23438
Type of resourceText
Access conditionsOpen access
Terms of useLicence In copyright icon
Created on2018-02-27 10:23:30