prikaz prve stranice dokumenta Co-occurrence of aflatoxins, ochratoxin A, fumonisins, and zearalenone in cereals and feed, determined by competitive direct enzyme-linked immunosorbent assay and thin-layer chromatography
  Download
PDF 163 KB
Scientific paper - Original scientific paper
Co-occurrence of aflatoxins, ochratoxin A, fumonisins, and zearalenone in cereals and feed, determined by competitive direct enzyme-linked immunosorbent assay and thin-layer chromatography
Arhiv za higijenu rada i toksikologiju, 60 (2009), 4; 427-434. https://doi.org/10.2478/10004-1254-60-2009-1975
Šegvić Klarić, Maja; Cvetnić, Zdenka; Pepeljnjak, Stjepan; Kosalec, Ivan

Institutional repository: Repository of Faculty of Pharmacy and Biochemistry University of Zagreb

Cite this document

Šegvić Klarić, M., Cvetnić, Z., Pepeljnjak, S. & Kosalec, I. (2009). Co-occurrence of aflatoxins, ochratoxin A, fumonisins, and zearalenone in cereals and feed, determined by competitive direct enzyme-linked immunosorbent assay and thin-layer chromatography. Arhiv za higijenu rada i toksikologiju, 60. (4), 427-434. doi: 10.2478/10004-1254-60-2009-1975

Šegvić Klarić, Maja, et al. "Co-occurrence of aflatoxins, ochratoxin A, fumonisins, and zearalenone in cereals and feed, determined by competitive direct enzyme-linked immunosorbent assay and thin-layer chromatography." Arhiv za higijenu rada i toksikologiju, vol. 60, no. 4, 2009, pp. 427-434. https://doi.org/10.2478/10004-1254-60-2009-1975

Šegvić Klarić, Maja, Zdenka Cvetnić, Stjepan Pepeljnjak and Ivan Kosalec. "Co-occurrence of aflatoxins, ochratoxin A, fumonisins, and zearalenone in cereals and feed, determined by competitive direct enzyme-linked immunosorbent assay and thin-layer chromatography." Arhiv za higijenu rada i toksikologiju 60, no. 4 (2009): 427-434. https://doi.org/10.2478/10004-1254-60-2009-1975

Šegvić Klarić, M., et al. (2009) 'Co-occurrence of aflatoxins, ochratoxin A, fumonisins, and zearalenone in cereals and feed, determined by competitive direct enzyme-linked immunosorbent assay and thin-layer chromatography', Arhiv za higijenu rada i toksikologiju, 60(4), pp. 427-434. doi: 10.2478/10004-1254-60-2009-1975

Šegvić Klarić M, Cvetnić Z, Pepeljnjak S, Kosalec I. Co-occurrence of aflatoxins, ochratoxin A, fumonisins, and zearalenone in cereals and feed, determined by competitive direct enzyme-linked immunosorbent assay and thin-layer chromatography. Arhiv za higijenu rada i toksikologiju [Internet]. 2009 December 22 [cited 2024 November 25];60(4):427-434. doi: 10.2478/10004-1254-60-2009-1975

M. Šegvić Klarić, Z. Cvetnić, S. Pepeljnjak and I. Kosalec, "Co-occurrence of aflatoxins, ochratoxin A, fumonisins, and zearalenone in cereals and feed, determined by competitive direct enzyme-linked immunosorbent assay and thin-layer chromatography", Arhiv za higijenu rada i toksikologiju, vol. 60, no. 4, pp. 427-434, December 2009. [Online]. Available at: https://urn.nsk.hr/urn:nbn:hr:163:950918. [Accessed: 25 November 2024]

Metadata
Title (english)Co-occurrence of aflatoxins, ochratoxin A, fumonisins, and zearalenone in cereals and feed, determined by competitive direct enzyme-linked immunosorbent assay and thin-layer chromatography
Title (croatian)Određivanje aflatoksina, okratoksina A, fumonizina i zearalenona u žitaricama i krmivu primjenom kompetetivnoga direktnog imunoenzimatskog testa (CD-ELISA) i tankoslojne kromatografije (TLC)
AuthorMaja Šegvić Klarić
AuthorZdenka Cvetnić
AuthorStjepan Pepeljnjak
AuthorIvan Kosalec
Author's institutionUniversity of Zagreb
Faculty of Pharmacy and Biochemistry
(Department of microbiology)
Scientific / art field,
discipline and subdiscipline		BIOMEDICINE AND HEALTHCARE
Pharmacy
Pharmacy
Abstract (english)
Aspergillus, Penicillium, and Fusarium species frequently contaminate crops. For this reason mycotoxins such as afl atoxins (AFs), ochratoxin A (OTA), fumonisins (FBs), and zearalenone (ZEA) are found in food and feed in a wide range of concentrations, depending on environmental and storage conditions. Consumption of mycotoxin-contaminated food and feed has been associated with acute and chronic poisoning and carcinoma. The aim of this study was to determine the incidence and co-occurrence of AFs (B1+B2+G1+G2), OTA, FBs (B1+B2+B3), and ZEA in 37 samples of cereals and feed randomly collected in 2007 from households of an endemic nephropathy (EN) area in Croatia. The mycotoxins were determined using the competitive direct ELISA test (CD-ELISA) in combination with thin-layer chromatography (TLC). The most frequent mycotoxin was ZEA (92 %, mean 318.3 μg kg-1), followed by FBs (27 %, 3690 μg kg-1), AFs (24.3 %, 4.6 μg kg-1), and OTA (16.2 %, 9.8 μg kg-1). Levels of AFs, ZEA, and FBs detected by CD-ELISA signifi cantly correlated with the TLC results. However, only one OTA-positive sample was confi rmed by TLC due to its high limit of detection. The levels of these mycotoxins were below the permissible limit for animal feed. Twenty-nine percent of cereals were contaminated with FBs, OTA, or ZEA in mass fractions above the permissible limit for humans. Co-occurrence of two toxins varied between 4.2 % and 54 % and of three between 4.2 % and 7.6 %. Prolonged co-exposure to AFs, OTA, FBs, and ZEA might increase the risk of various chronic diseases.
Abstract (croatian)
Vrste plijesni iz rodova Aspergillus, Penicillium i Fusarium česti su kontaminanti usjeva te na takvim supstratima tvore mikotoksine. Stoga su žitarice i krmiva često kontaminirana afl atoksinima (AFs), okratoksinom A (OTA), fumonizinima (FBs) i zearalenonom (ZEA) u različitim koncentracijama ovisno o mikroklimatskim uvjetima na polju i u skladištu. Konzumiranje hrane kontaminirane mikotoksinima često je povezano s akutnim ili kroničnim trovanjima, ali i s razvojem karcinoma. Cilj ovog rada bio je odrediti istodobnu pojavnost AFs (B1+B2+G1+G2), OTA, FBs (B1+B2+B3) i ZEA u uzorcima žitarica i krme (N=37) koji su nasumično skupljeni u individualnim domaćinstvima na području endemske nefropatije (EN) u Hrvatskoj (2007). Za određivanje navedenih mikotoksina korišten je kompetitivni direktni ELISA-test (CD-ELISA) u kombinaciji s tankoslojnom kromatografi jom (TLC). Najzastupljeniji mikotoksin bio je ZEA (92 %, srednja koncentracija 318.3 μg kg-1), nakon čega slijede FBs (27 %, 3690 μg kg-1), AFs (24.3 %, 4.6 μg kg-1) te OTA (16.2 %, 9.8 μg kg-1). Koncentracije AFs, FBs i ZEA određene CD-ELISA-testom statistički značajno koreliraju s rezultatima dobivenim s TLC. OTA je potvrđen metodom TLC samo u jednom uzorku zbog visokog limita detekcije. Dokazane koncentracije su ispod razina dopuštenih za krmiva, dok je 29 % uzoraka žitarica sadržavalo FBs, OTA ili ZEA u koncentracijama iznad dopuštenih u hrani za ljude. Kokontaminacija s dvama odnosno trima toksinima varirala je između 4.2 % i 54 % odnosno između 4.2 % i 7.6 %. Dugotrajni unos AFs, OTA, FBs i ZEA putem hrane može povećati rizik od razvoja različitih kroničnih bolesti zbog njihova mogućega sinergističkog djelovanja.
Keywords (english)
Keywords (croatian)
Languageenglish
Publication typeScientific paper - Original scientific paper
Publication statusPublished
Peer reviewPeer review - international
Publication versionPublished version
Journal titleArhiv za higijenu rada i toksikologiju
Numberingvol. 60, no. 4, pp. 427-434
p-ISSN0004-1254
e-ISSN1848-6312
DOIhttps://doi.org/10.2478/10004-1254-60-2009-1975
URN:NBNurn:nbn:hr:163:950918
Publication2009-12-22
Document URLhttps://hrcak.srce.hr/45377
Type of resourceText
Access conditionsOpen access
Terms of useLicence In copyright icon
Created on2018-02-27 11:19:32