Zinc is one of the essential microelements important for the development of human body and participates in the growth and development of cells as part of many enzymes. At the organism level it is the key in processes of normal function of the immune system and small changes in the amount can lead to a greater number of physiological disorders. Therefore, it is necessary to have a method that allows a quick, accurate and reproducible measurement of its quantity. For this purpose, Atomic Absorption Spectrometry (AAS) has been used as the well-established and widely used method together with the Total Reflection X-ray Fluorescence Spectrometry (TXRF), which has become increasingly used in recent years and has the potential to enable even faster, simpler, multi-elemental analysis in nanogram quantities at a low cost of maintenance. By carrying out a brief analytical validation of the atomic absorption spectrophotometry method, as well as the total reflection x-ray fluorescence spectrometry method for determining urine zinc concentrations, it has been shown that the eligibility criteria for accuracy, precision and linearity have been met. Comparison with the existing method of determination of zinc concentrations in urine Bland-Altman analysis showed that there is a proportional and constant deviation between the methods, that is, that they are not comparable. Despite the fact that the methods are not comparable, due to the many advantages that include the analytical characteristics of the TXRF technique, it is increasingly applied to a wide spectrum of samples.