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master's thesis
Optimiranje uvjeta analize S-fenilmerkapturne kiseline u mokraći primjenom vezanog sustava plinski kromatograf-spektrometar masa
Zagreb: University of Zagreb, Faculty of Science, 2021. urn:nbn:hr:217:466382
Cindrić, Doroteja
University of Zagreb
Faculty of Science
Department of Chemistry

Institutional repository: Repository of the Faculty of Science

Cite this document

Cindrić, D. (2021). Optimiranje uvjeta analize S-fenilmerkapturne kiseline u mokraći primjenom vezanog sustava plinski kromatograf-spektrometar masa (Master's thesis). Zagreb: University of Zagreb, Faculty of Science. Retrieved from https://urn.nsk.hr/urn:nbn:hr:217:466382

Cindrić, Doroteja. "Optimiranje uvjeta analize S-fenilmerkapturne kiseline u mokraći primjenom vezanog sustava plinski kromatograf-spektrometar masa." Master's thesis, University of Zagreb, Faculty of Science, 2021. https://urn.nsk.hr/urn:nbn:hr:217:466382

Cindrić, Doroteja. "Optimiranje uvjeta analize S-fenilmerkapturne kiseline u mokraći primjenom vezanog sustava plinski kromatograf-spektrometar masa." Master's thesis, University of Zagreb, Faculty of Science, 2021. https://urn.nsk.hr/urn:nbn:hr:217:466382

Cindrić, D. (2021). 'Optimiranje uvjeta analize S-fenilmerkapturne kiseline u mokraći primjenom vezanog sustava plinski kromatograf-spektrometar masa', Master's thesis, University of Zagreb, Faculty of Science, accessed 09 November 2024, https://urn.nsk.hr/urn:nbn:hr:217:466382

Cindrić D. Optimiranje uvjeta analize S-fenilmerkapturne kiseline u mokraći primjenom vezanog sustava plinski kromatograf-spektrometar masa [Master's thesis]. Zagreb: University of Zagreb, Faculty of Science; 2021 [cited 2024 November 09] Available at: https://urn.nsk.hr/urn:nbn:hr:217:466382

D. Cindrić, "Optimiranje uvjeta analize S-fenilmerkapturne kiseline u mokraći primjenom vezanog sustava plinski kromatograf-spektrometar masa", Master's thesis, University of Zagreb, Faculty of Science, Zagreb, 2021. Available at: https://urn.nsk.hr/urn:nbn:hr:217:466382

Metadata
TitleOptimiranje uvjeta analize S-fenilmerkapturne kiseline u mokraći primjenom vezanog sustava plinski kromatograf-spektrometar masa
Title (english)Optimising the conditions for S-phenylmercapturic acid analysis in urine using gas chromatography-mass spectrometry
AuthorDoroteja Cindrić
MentorIrena Brčić Karačonji (mentor)
Committee memberIva Juranović Cindrić (predsjednik povjerenstva)
Committee memberIvica Đilović (član povjerenstva)
Committee memberĐani Škalamera (član povjerenstva)
Committee memberAdriana Kenđel (član povjerenstva)
GranterUniversity of Zagreb
Faculty of Science
(Department of Chemistry)
Zagreb
Defense date and country2021-09-29, Croatia
Scientific / art field,
discipline and subdiscipline		NATURAL SCIENCES
Chemistry
Abstract
Benzen je aromatski spoj s kancerogenim svojstvima kojem su ljudi, osim profesionalno, izloženi putem cigaretnog dima ili ispušnih plinova motornih vozila. S-Fenilmerkapturna kiselina (S-PMA) smatra se specifičnim pokazateljem izloženosti benzenu kod opće populacije i pri profesionalnoj izloženosti. Cilj ovog istraživanja bio je optimizirati uvjete ekstrakcije SPMA iz mokraće te razviti i validirati osjetljivu i specifičnu analitičku metodu za određivanje masene koncentracije S-PMA u mokraći primjenom vezanog sustava plinski kromatografspektrometar masa. Predložena metoda je pokazala dobru linearnost (R2>0,9996) u ispitivanom koncentracijskom rasponu (0,5 – 100 μg L-1), preciznost (RSD<7 %), točnost (>95 %) i osjetljivost (granica detekcije: 0,03 μg L-1i granica kvantifikacije: 0,09 μg L-1). Metoda je primijenjena za određivanje masene koncentracije S-PMA u mokraći 20 ispitanika koji nisu bili profesionalno izloženi benzenu. Pušači (N=10) su imali značajno višu koncentraciju S-PMA u mokraći u usporedbi s nepušačima (N=10).
Abstract (english)
Benzene is an aromatic compound with carcinogenic properties to which people, except in professional settings, are exposed by cigarette smoke or motor vehicle exhaust. Sphenylmercapturic acid (S-PMA) is considered a specific marker of benzene exposure in the general and occupationally exposed population. The aim of this study was to optimise the conditions of S-PMA extraction from urine and to develop and validate a sensitive and specific analytical method for determining the mass concentration of S-PMA in urine using gas chromatography-mass spectrometry. The proposed method showed good linearity (R2> 0,9996) in the examined concentration range (0,5 – 100 μg L-1), good precision (RSD <7%), accuracy (> 95%) and sensitivity (limit of detection: 0,03 μg L-1 1 and limit of quantification: 0,09 μg L1). The method was applied to determine the mass concentration of S-PMA in the urine of 20 subjects who were not occupationally exposed to benzene. Smokers (N = 10) had a significantly higher concentration of S-PMA in urine compared to non-smokers (N = 10).
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:217:466382
Study programmeTitle: Graduate university studies in chemistry; specializations in: Research programme
Course: Research programme
Study programme type: university
Study level: graduate
Academic / professional title: magistar/magistra kemije (magistar/magistra kemije)
Type of resourceText
File originBorn digital
Access conditionsOpen access
Embargo expiration date: 2023-09-29
Terms of useLicence In copyright icon
Created on2021-10-18 09:55:09