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master's thesis
Odabir fiksativa za procjenu enzimske aktivnosti u citokemijskim testovima leukemija
Zagreb: University of Zagreb, Faculty of Science, 2021. urn:nbn:hr:217:079805
Dunđer, Nikolina
University of Zagreb
Faculty of Science
Department of Biology

Institutional repository: Repository of the Faculty of Science

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Dunđer, N. (2021). Odabir fiksativa za procjenu enzimske aktivnosti u citokemijskim testovima leukemija (Master's thesis). Zagreb: University of Zagreb, Faculty of Science. Retrieved from https://urn.nsk.hr/urn:nbn:hr:217:079805

Dunđer, Nikolina. "Odabir fiksativa za procjenu enzimske aktivnosti u citokemijskim testovima leukemija." Master's thesis, University of Zagreb, Faculty of Science, 2021. https://urn.nsk.hr/urn:nbn:hr:217:079805

Dunđer, Nikolina. "Odabir fiksativa za procjenu enzimske aktivnosti u citokemijskim testovima leukemija." Master's thesis, University of Zagreb, Faculty of Science, 2021. https://urn.nsk.hr/urn:nbn:hr:217:079805

Dunđer, N. (2021). 'Odabir fiksativa za procjenu enzimske aktivnosti u citokemijskim testovima leukemija', Master's thesis, University of Zagreb, Faculty of Science, accessed 13 May 2024, https://urn.nsk.hr/urn:nbn:hr:217:079805

Dunđer N. Odabir fiksativa za procjenu enzimske aktivnosti u citokemijskim testovima leukemija [Master's thesis]. Zagreb: University of Zagreb, Faculty of Science; 2021 [cited 2024 May 13] Available at: https://urn.nsk.hr/urn:nbn:hr:217:079805

N. Dunđer, "Odabir fiksativa za procjenu enzimske aktivnosti u citokemijskim testovima leukemija", Master's thesis, University of Zagreb, Faculty of Science, Zagreb, 2021. Available at: https://urn.nsk.hr/urn:nbn:hr:217:079805

Metadata
TitleOdabir fiksativa za procjenu enzimske aktivnosti u citokemijskim testovima leukemija
Title (english)Selection of fixatives to assess enzyme activity in cytochemical leukemia assays
AuthorNikolina Dunđer
MentorMaja Šantak (mentor)
MentorNada Oršolić (sumentor)
Committee memberNada Oršolić (predsjednik povjerenstva)
Committee memberJasna Lajtner (član povjerenstva)
Committee memberGoran Kovačević (član povjerenstva)
GranterUniversity of Zagreb
Faculty of Science
(Department of Biology)
Zagreb
Defense date and country2021-09-28, Croatia
Scientific / art field,
discipline and subdiscipline		NATURAL SCIENCES
Biology
Abstract
Najčešće fiksirajuće sredstvo u citokemijskim testovima za dijagnostiku leukemija je kombinacija acetona i formalina. Unatoč svojoj širokoj uporabi, formalin je kancerogen i mutagen, stoga se njegova uporaba nastoji izbaciti ili svesti na minimum. Trenutno ne postoji adekvatna zamjena formalina, stoga je glavni cilj ovog rada izraditi probne formulacije fiksativa za citokemijsku dijagnostiku leukemija koji neće sadržavati formalin i koji neće imati štetno djelovanje, a koji će dati jednako dobre rezultate kao fiksativ s formalinom. Napravljeno je 36 probnih formulacija, od kojih je 25 sadržavalo manje štetan dialdehid glioksal uz dodatak suplementa, a ostale nove formulacije nisu uopće sadržavale aldehidne fiksative. Od napravljenih 36 uzoraka njih 8 je fiksiralo stanice usporedivo s fiksativom koji sadrži formalin te su odabrani kao probni fiksativi u citokemijskim testovima za dijagnostiku leukemija. Šest probnih fiksativa je pokazalo dobre rezultate pri bojenju kompletima za detekciju leukemija osim bojenja s kompletom Sudan Black B Eco. No jedna od preostale dvije nove formulacija koje nisu dale dobar rezultat s ostalim testovima za dijagnostiku leukemija je pokazala dobar rezultat s kompletom Sudan Black B Eco.
Abstract (english)
The most common fixative in cytochemical tests for the diagnosis of leukemia is a combination of acetone and formalin. Despite its widespread use, formalin is carcinogenic and mutagenic, therefore its use seeks to be eliminated or minimized. There is currently no adequate replacement for formalin, therefore the main goal of this paper is to develop trial formulations of fixatives for cytochemical diagnosis of leukemia that will not contain formalin and that will not have a harmful effect, and which will give just as good results as a formalin fixative. 36 trial formulations were made, from which 25 contained the less harmful dialdehyde glyoxal with the addition of a supplement, and the other new formulations did not contain aldehyde fixatives at all. Of the 36 samples, 8 of them fixed cells comparable to a formalin-containing fixative and were selected as test fixatives in cytochemical tests for the diagnosis of leukemia. Six trial fixatives showed good results in staining with leukemia detection kits in addition to staining with the Sudan Black B Eco kit. But one of the remaining two new formulations that did not give a good result with other tests for the diagnosis of leukemia showed a good result with the Sudan Black B Eco kit
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:217:079805
Study programmeTitle: Experimental Biology
Study programme type: university
Study level: graduate
Academic / professional title: magistar/magistra eksperimentalne biologije (magistar/magistra eksperimentalne biologije)
Type of resourceText
File originBorn digital
Access conditionsOpen access
Terms of useLicence In copyright icon
Created on2021-10-25 06:47:02