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master's thesis
Karakterizacija strukturnih obilježja valovite faze fosfokolinskoh liposoma FT-IR spektroskopijom
Zagreb: University of Zagreb, Faculty of Science, 2021. urn:nbn:hr:217:739690
Pišonić, Marina
University of Zagreb
Faculty of Science
Department of Chemistry

Institutional repository: Repository of the Faculty of Science

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Pišonić, M. (2021). Karakterizacija strukturnih obilježja valovite faze fosfokolinskoh liposoma FT-IR spektroskopijom (Master's thesis). Zagreb: University of Zagreb, Faculty of Science. Retrieved from https://urn.nsk.hr/urn:nbn:hr:217:739690

Pišonić, Marina. "Karakterizacija strukturnih obilježja valovite faze fosfokolinskoh liposoma FT-IR spektroskopijom." Master's thesis, University of Zagreb, Faculty of Science, 2021. https://urn.nsk.hr/urn:nbn:hr:217:739690

Pišonić, Marina. "Karakterizacija strukturnih obilježja valovite faze fosfokolinskoh liposoma FT-IR spektroskopijom." Master's thesis, University of Zagreb, Faculty of Science, 2021. https://urn.nsk.hr/urn:nbn:hr:217:739690

Pišonić, M. (2021). 'Karakterizacija strukturnih obilježja valovite faze fosfokolinskoh liposoma FT-IR spektroskopijom', Master's thesis, University of Zagreb, Faculty of Science, accessed 21 December 2024, https://urn.nsk.hr/urn:nbn:hr:217:739690

Pišonić M. Karakterizacija strukturnih obilježja valovite faze fosfokolinskoh liposoma FT-IR spektroskopijom [Master's thesis]. Zagreb: University of Zagreb, Faculty of Science; 2021 [cited 2024 December 21] Available at: https://urn.nsk.hr/urn:nbn:hr:217:739690

M. Pišonić, "Karakterizacija strukturnih obilježja valovite faze fosfokolinskoh liposoma FT-IR spektroskopijom", Master's thesis, University of Zagreb, Faculty of Science, Zagreb, 2021. Available at: https://urn.nsk.hr/urn:nbn:hr:217:739690

Metadata
TitleKarakterizacija strukturnih obilježja valovite faze fosfokolinskoh liposoma FT-IR spektroskopijom
Title (english)Characterization of the structural features of ripple phase of phosphocholine liposomes using FT-IR spectroscopy
AuthorMarina Pišonić
MentorDanijela Bakarić (mentor)
Committee memberSnežana Miljanić (predsjednik povjerenstva)
Committee memberŽeljka Soldin (član povjerenstva)
Committee memberVesna Petrović Peroković (član povjerenstva)
Committee memberAdriana Kenđel (član povjerenstva)
GranterUniversity of Zagreb
Faculty of Science
(Department of Chemistry)
Zagreb
Defense date and country2021-09-30, Croatia
Scientific / art field,
discipline and subdiscipline		NATURAL SCIENCES
Chemistry
Abstract
Molekule 1,2-dipalmitoil-sn-glicero-3-fosfokolina (engl. 1,2-dipalmitoyl-sn-glicero-3- phosphatidylcholine, DPPC) u vodenom mediju stvaraju hidrirane dvosloje koji se, ovisno o temperaturi, nalaze u određenoj fazi. Prijelaz DPPC-a iz faze gela (Lβ') u fazu fluida (Lα), odnosno glavni fazni prijelaz opažen pri temperaturi mekšanja (Tm), prekinut je valovitom fazom (Pβ') koja se opaža pri temperaturi pretprijelaza (Tp). Budući da su Tm i Tp iznimno osjetljive na ugradnju organskih molekula u lipidni dvosloj, posebice onih koje su kadre primati i otpuštati proton, u sklopu ovog diplomskog rada istraženi su višestruki hidrirani dvosloji DPPC lipida u odsutnosti i prisutnosti male količine palmitinske kiseline (engl. palmitic acid, PA) u puferima različitih pH vrijednosti. Tm i Tp određene su analizom vibracijskih potpisa metilenskih (νsCH2) i fosfatnih (νasPO2−) skupina iz temperaturno-ovisnih FT-IR spektara. Dobiveni podaci su djelomično upotpunjeni onima dobivenima iz razlikovno-pretražne kalorimetrije (engl. differential scanning calorimetry, DSC).
Abstract (english)
1,2-dipalmitoyl-sn-glycero-3-phosphocoline form hydrated bilayers in an aqueous medium which, depending on the temperature, can be found in certain phase. The transition from gel (Lβ') to fluid phase (Lα), assigned as the main phase transition with a peak at melting point temperature (Tm), is interrupted by the ripple phase (Pβ') observed at the pretransition temeprature (Tp). Since Tm and Tp are extremely sensitive to the incorporation of organic molecules into the lipid bilayer, especially those capable of receiving and releasing a proton, multiple hydrated bilayers of DPPC lipids in the absence and presence of small amounts of palmitic acid (PA) in buffers of different pH values have been investigated across this thesis. Tm and Tp have been determined by the analysis of vibrational signatures of methylene (νsCH2) and phosphate (νasPO2−) groups from temperature-dependent FT-IR spectra. These data have been partially supplemented by those obtained from differential scanning calorimetry (DSC).
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:217:739690
ProjectNumber: UIP-2014-09-6090
Title: Molekularni aspekti oksidativnih procesa u stanicama
Title: Molecular Aspects of Oxidative Processes in Cells
Acronym: MolOxStress
Leader: Mario Vazdar
Jurisdiction: Croatia
Funder: HRZZ
Funding stream: UIP
ProjectNumber: IP-2019-04-3804
Title: Prijenos tvari kroz biološke membrane potpomognut proteinima
Title: Protein Assisted Translocation of Matter across Biological Membranes
Acronym: ProtBioMemb
Leader: Mario Vazdar
Jurisdiction: Croatia
Funder: HRZZ
Funding stream: IP
Study programmeTitle: Graduate university studies in chemistry; specializations in: Research programme
Course: Research programme
Study programme type: university
Study level: graduate
Academic / professional title: magistar/magistra kemije (magistar/magistra kemije)
Type of resourceText
File originBorn digital
Access conditionsOpen access
Embargo expiration date: 2023-09-30
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Created on2021-10-26 14:00:41