prikaz prve stranice dokumenta Združeni učinak anestetika sevoflurana i ionizirajućeg zračenja na primarna oštećenja DNA u miša
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master's thesis
Združeni učinak anestetika sevoflurana i ionizirajućeg zračenja na primarna oštećenja DNA u miša
Zagreb: University of Zagreb, Faculty of Science, 2012. urn:nbn:hr:217:369599
Halovanić, Sanja
University of Zagreb
Faculty of Science
Department of Biology

Institutional repository: Repository of the Faculty of Science

Cite this document

Halovanić, S. (2012). Združeni učinak anestetika sevoflurana i ionizirajućeg zračenja na primarna oštećenja DNA u miša (Master's thesis). Zagreb: University of Zagreb, Faculty of Science. Retrieved from https://urn.nsk.hr/urn:nbn:hr:217:369599

Halovanić, Sanja. "Združeni učinak anestetika sevoflurana i ionizirajućeg zračenja na primarna oštećenja DNA u miša." Master's thesis, University of Zagreb, Faculty of Science, 2012. https://urn.nsk.hr/urn:nbn:hr:217:369599

Halovanić, Sanja. "Združeni učinak anestetika sevoflurana i ionizirajućeg zračenja na primarna oštećenja DNA u miša." Master's thesis, University of Zagreb, Faculty of Science, 2012. https://urn.nsk.hr/urn:nbn:hr:217:369599

Halovanić, S. (2012). 'Združeni učinak anestetika sevoflurana i ionizirajućeg zračenja na primarna oštećenja DNA u miša', Master's thesis, University of Zagreb, Faculty of Science, accessed 30 December 2024, https://urn.nsk.hr/urn:nbn:hr:217:369599

Halovanić S. Združeni učinak anestetika sevoflurana i ionizirajućeg zračenja na primarna oštećenja DNA u miša [Master's thesis]. Zagreb: University of Zagreb, Faculty of Science; 2012 [cited 2024 December 30] Available at: https://urn.nsk.hr/urn:nbn:hr:217:369599

S. Halovanić, "Združeni učinak anestetika sevoflurana i ionizirajućeg zračenja na primarna oštećenja DNA u miša", Master's thesis, University of Zagreb, Faculty of Science, Zagreb, 2012. Available at: https://urn.nsk.hr/urn:nbn:hr:217:369599

Metadata
TitleZdruženi učinak anestetika sevoflurana i ionizirajućeg zračenja na primarna oštećenja DNA u miša
Title (english)The combined effect of anaesthetic sevoflurane and ionising radiation on primary DNA damage in mice
AuthorSanja Halovanić
MentorVesna Benković (mentor)
Committee memberVesna Benković (predsjednik povjerenstva)
Committee memberZora Popović (član povjerenstva)
Committee memberZdravko Dolenec (član povjerenstva)
Committee memberVesna Petrović Peroković (član povjerenstva)
GranterUniversity of Zagreb
Faculty of Science
(Department of Biology)
Zagreb
Defense date and country2012-12-13, Croatia
Scientific / art field,
discipline and subdiscipline		NATURAL SCIENCES
Biology
Abstract
Sevofluran, fluorometil-heksafluoroizopropil-eter, je inhalacijski anestetik, a slaba topljivost i neiritabilnost omogućuju mu široku primjenu u uvodu i održavanju opće anestezije. Ionizirajuće zračenje ima široku primjenu u medicini (u radioterapiji). Cilj istraživanja je utvrditi i usporediti međudjelovanja γ-zračenja (60Co) u klinički relevantnoj dozi (1 Gy) i anestetika sevoflurana (2,4%, 50:50) na modelu miša soja Swiss albino, s naglaskom na proučavanje stupnja osjetljivosti različitih vrsta tkiva (mozga, jetre i bubrega) i leukocita periferne krvi. Za procjenu primarnih oštećenja DNA, primijenjena je metoda alkalnog komet testa neposredno nakon, te 2, 6 i 24 h od izlaganja anesteziji i zračenju. Najveći genotoksični učinak izmjeren je u stanicama bubrega i mozga 2 sata, u krvi 6 sati, a u jetri 24 sata nakon tretmana životinja u odnosu na kontrolnu skupinu. Za ispitivanje razlika u količini popravka između krvi i različitih tkiva, izračunat je stanični indeks popravka (CRI – od engl. cellular DNA repair index), koji je pokazao različitu osjetljivost organa.
Abstract (english)
Sevoflurane (hexafluoroisopropyl fluoromethyl ether) is inhalation anaesthetic which low solubility and non-irritability, provides its wide application in induction and maintenance of general anesthesia. Ionising radiation is widely used in medicine (radiotherapy). The aim of the study was to determine and compare the interaction of γ-radiation (60Co) in a clinically relevant dose (1 Gy) and anaesthetic sevoflurane (2.4%, 50:50) in the model of Swiss albino mices, with an emphasis on the study of the degree of sensitivity of different types of tissue (brain, liver and kidneys), and peripheral blood leukocytes. For the assessment of primary DNA damage, the method of alkaline comet assay was applied immediately after, and 2, 6 and 24 h of exposure to radiation and anaesthesia. The strongest genotoxic effect was measured in kidney and brain cells 2 hours, in the blood 6 hours, and in the liver 24 hours after the treatment of the animals as compared to a control group. To test the difference in the amount of repair between blood and various tissues, cellular DNA repair index (CRI) was calculated, which showed different sensitivity of organs.
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:217:369599
Study programmeTitle: Integrated undergraduate and graduate university study of Biology and Chemistry Education; specializations in: Integrated undergraduate and graduate university study of Biology and Chemistry Education
Course: Integrated undergraduate and graduate university study of Biology and Chemistry Education
Study programme type: university
Study level: integrated undergraduate and graduate
Academic / professional title: magistar/magistra edukacije biologije i kemije (magistar/magistra edukacije biologije i kemije)
Type of resourceText
File originBorn digital
Access conditionsAccess restricted to students and staff of home institution
Terms of useLicence In copyright icon
Created on2017-05-02 08:00:59