prikaz prve stranice dokumenta Učinak biljnih hormona stresa na klijance kineskog kupusa (Brassica rapa L. ssp. pekinensis)
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master's thesis
Učinak biljnih hormona stresa na klijance kineskog kupusa (Brassica rapa L. ssp. pekinensis)
Zagreb: University of Zagreb, Faculty of Science, 2012. urn:nbn:hr:217:852354
Janković, Iva
University of Zagreb
Faculty of Science
Department of Biology

Institutional repository: Repository of the Faculty of Science

Cite this document

Janković, I. (2012). Učinak biljnih hormona stresa na klijance kineskog kupusa (Brassica rapa L. ssp. pekinensis) (Master's thesis). Zagreb: University of Zagreb, Faculty of Science. Retrieved from https://urn.nsk.hr/urn:nbn:hr:217:852354

Janković, Iva. "Učinak biljnih hormona stresa na klijance kineskog kupusa (Brassica rapa L. ssp. pekinensis)." Master's thesis, University of Zagreb, Faculty of Science, 2012. https://urn.nsk.hr/urn:nbn:hr:217:852354

Janković, Iva. "Učinak biljnih hormona stresa na klijance kineskog kupusa (Brassica rapa L. ssp. pekinensis)." Master's thesis, University of Zagreb, Faculty of Science, 2012. https://urn.nsk.hr/urn:nbn:hr:217:852354

Janković, I. (2012). 'Učinak biljnih hormona stresa na klijance kineskog kupusa (Brassica rapa L. ssp. pekinensis)', Master's thesis, University of Zagreb, Faculty of Science, accessed 22 November 2024, https://urn.nsk.hr/urn:nbn:hr:217:852354

Janković I. Učinak biljnih hormona stresa na klijance kineskog kupusa (Brassica rapa L. ssp. pekinensis) [Master's thesis]. Zagreb: University of Zagreb, Faculty of Science; 2012 [cited 2024 November 22] Available at: https://urn.nsk.hr/urn:nbn:hr:217:852354

I. Janković, "Učinak biljnih hormona stresa na klijance kineskog kupusa (Brassica rapa L. ssp. pekinensis)", Master's thesis, University of Zagreb, Faculty of Science, Zagreb, 2012. Available at: https://urn.nsk.hr/urn:nbn:hr:217:852354

Metadata
TitleUčinak biljnih hormona stresa na klijance kineskog kupusa (Brassica rapa L. ssp. pekinensis)
Title (english)Plant stress hormones effect on the Chinese cabbage seedlings (Brassica rapa L. ssp. pekinensis)
AuthorIva Janković
MentorBranka Salopek Sondi (mentor)
MentorBiljana Balen (mentor)
Committee memberBiljana Balen (predsjednik povjerenstva)
Committee memberBranka Salopek Sondi (član povjerenstva)
Committee memberSandra Radić Brkanac (član povjerenstva)
GranterUniversity of Zagreb
Faculty of Science
(Department of Biology)
Zagreb
Defense date and country2012-02-14, Croatia
Scientific / art field,
discipline and subdiscipline		NATURAL SCIENCES
Biology
Abstract
Biljni hormoni auksini neophodni su u regulaciji biljnog razvitka kao i odgovoru biljaka na čimbenike stresa. Auksini se u biljnom tkivu pojavljuju slobodni (aktivne forme) ili konjugirani s aminokiselinama i šećerima (skladišne i transportne forme). Iako precizni mehanizam održavanja homeostaze auksina još nije razjašnjen, pretpostavlja se da glavnu ulogu imaju procesi konjugacije te hidrolize konjugiranih auksina po potrebi (pomoću enzima auksin-amidohidrolaza). Da bismo dobili uvid u interakcije hormona stresa i auksinskog metabolizma, u ovom radu istraţen je učinak hormona stresa: salicilne kiseline (SA), jasmonske kiseline (JA) i apscizinske kiseline (ABA) na klijance kineskog kupusa (Brassica rapa L. ssp. pekinensis). Koristeći biotest inhibicije korijena in vivo utvrđeno je da hormoni stresa djeluju inhibitorno na rast korijena uglavnom ovisno o koncentraciji. Na genskoj razini je metodom lančane reakcije polimerazom u stvarnom vremenu utvrđeno stimulativno djelovanje JA i SA na ekspresiju transkripta za auksin-amidohidrolazu BrILL2, dok je ABA djelovala inhibitorno. Spektrofotometrijskim metodama izmjereno je oksidacijsko stanje klijanaca te se pokazalo da ne dolazi do značajnog oksidacijskog stresa uslijed tretmana hormonima stresa.
Abstract (english)
Plant hormones auxins play important role in regulation of plant development as well as plant response to stress. Auxins can be found in plants as free forms (active forms), and conjugated with amino acids or sugars (storage and transport forms). Although the precise mechanism of auxin homeostasis is not fully discovered, it is assumed that their homeostasis is regulated by processes of conjugation, and hydrolysis upon tissue demand (which is accomplished by auxin amidohydrolases enzymes). In order to gain information about influence of stress hormones on the auxin homeostasis, the effect of stress hormones: salicylic acid (SA), jasmonic acid (JA), and abscisic acid (ABA) was investigated on the Chinese cabbage seedlings (Brassica rapa L. ssp. pekinensis). Inhibition of root growth upon treatment with stress hormones was identified in vivo, mostly in a dose dependent manner, using root growth bioassay. An expression level of auxin amidohydrolase BrILL2 transcript upon treatments was examined by RT-PCR. JA and SA caused up regulation, while ABA caused down regulation of BrILL2 transcript expression. Finally, oxidation status of Chinese cabbage seedlings upon treatments was discovered using spectrophotometric methods and it was found that stress hormones do not significantly affect oxidation status of seedlings.
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:217:852354
Study programmeTitle: Molecular Biology
Study programme type: university
Study level: graduate
Academic / professional title: magistar/magistra molekularne biologije (magistar/magistra molekularne biologije)
Type of resourceText
File originBorn digital
Access conditionsAccess restricted to students and staff of home institution
Terms of useLicence In copyright icon
Created on2017-05-02 08:18:00