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master's thesis
Ispitivanje interakcija halogeniranih i nehalogeniranih derivata cijanina s dvolančanim DNA i RNA spektroskopskim metodama
Zagreb: University of Zagreb, Faculty of Science, 2021. urn:nbn:hr:217:889313
Mihovilović, Petra
University of Zagreb
Faculty of Science
Department of Chemistry

Institutional repository: Repository of the Faculty of Science

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Mihovilović, P. (2021). Ispitivanje interakcija halogeniranih i nehalogeniranih derivata cijanina s dvolančanim DNA i RNA spektroskopskim metodama (Master's thesis). Zagreb: University of Zagreb, Faculty of Science. Retrieved from https://urn.nsk.hr/urn:nbn:hr:217:889313

Mihovilović, Petra. "Ispitivanje interakcija halogeniranih i nehalogeniranih derivata cijanina s dvolančanim DNA i RNA spektroskopskim metodama." Master's thesis, University of Zagreb, Faculty of Science, 2021. https://urn.nsk.hr/urn:nbn:hr:217:889313

Mihovilović, Petra. "Ispitivanje interakcija halogeniranih i nehalogeniranih derivata cijanina s dvolančanim DNA i RNA spektroskopskim metodama." Master's thesis, University of Zagreb, Faculty of Science, 2021. https://urn.nsk.hr/urn:nbn:hr:217:889313

Mihovilović, P. (2021). 'Ispitivanje interakcija halogeniranih i nehalogeniranih derivata cijanina s dvolančanim DNA i RNA spektroskopskim metodama', Master's thesis, University of Zagreb, Faculty of Science, accessed 30 December 2024, https://urn.nsk.hr/urn:nbn:hr:217:889313

Mihovilović P. Ispitivanje interakcija halogeniranih i nehalogeniranih derivata cijanina s dvolančanim DNA i RNA spektroskopskim metodama [Master's thesis]. Zagreb: University of Zagreb, Faculty of Science; 2021 [cited 2024 December 30] Available at: https://urn.nsk.hr/urn:nbn:hr:217:889313

P. Mihovilović, "Ispitivanje interakcija halogeniranih i nehalogeniranih derivata cijanina s dvolančanim DNA i RNA spektroskopskim metodama", Master's thesis, University of Zagreb, Faculty of Science, Zagreb, 2021. Available at: https://urn.nsk.hr/urn:nbn:hr:217:889313

Metadata
TitleIspitivanje interakcija halogeniranih i nehalogeniranih derivata cijanina s dvolančanim DNA i RNA spektroskopskim metodama
Title (english)Study of interactions of halogenated and non-halogenated cyanine derivatives with double-stranded DNA and RNA by spectrosopic methods
AuthorPetra Mihovilović
MentorMarijana Radić Stojković (mentor)
Committee memberIva Juranović Cindrić (predsjednik povjerenstva)
Committee memberInes Primožič (član povjerenstva)
Committee memberMarko Močibob (član povjerenstva)
Committee memberTomislav Jednačak (član povjerenstva)
GranterUniversity of Zagreb
Faculty of Science
(Department of Chemistry)
Zagreb
Defense date and country2021-10-22, Croatia
Scientific / art field,
discipline and subdiscipline		NATURAL SCIENCES
Chemistry
Abstract
Cijaninske boje, kao poznate fluorescentne probe, pronalaze primjenu u specifičnoj detekciji nukleinskih kiselina. U okviru ovog diplomskog rada spektroskopski su okarakterizirane tri novosintetizirane cijaninske boje. Otopine spojeva posjeduju karakteristični UV/Vis spektar u valnom području od 220 nm do 600 nm. Intrinzično nefluorescentni cijanini, odlikuju se izrazitim fluorimetrijskim odgovorom uslijed vezanja na polinukleotide. Interakcije cijaninskih boja s dvolančanim polinukleotidima ispitane su standardnim spektrofotometrijskim metodama. Eksperimentima temperaturnog mekšanja i titracijama metodom cirkularnog dikroizma utvrđen je način vezanja spojeva na polinukleotide. Obradom fluorimetrijskih titracija pomoću Scatchardove jednadžbe metodom nelinearne regresije dobivene su konstante stabilnosti kompleksa cijaninskih boja s polinukleotidima. Cijanini su pokazali selektivnost u odnosu na na sekvencu i sekundarnu strukturu nukleinskih kiselina. Dobiveni rezultati ukazuju na mješovit način vezanja cijaninskih boja, u kojemu uz interkalaciju dolazi do vezanja dimera ili većih agregata u utore polinukleotida
Abstract (english)
Cyanine dyes, as known fluorescent probes, find application in the specific detection of nucleic acids. Three newly synthesized cyanine dyes were characterized spectroscopically as part of this thesis. Aqueous solutions of compounds absorbed in the UV/Vis region 220-600 nm. Intrinsically non-fluorescent cyanines are characterized by a pronounced fluorimetric response upon binding to polynucleotides. Interactions of cyanine dyes with double-stranded polynucleotides were examined by standard spectrophotometric methods. The mode of binding of compounds to polynucleotides was determined by temperature melting experiments and CD titrations. Stability constants of the complexes with polynucleotides were obtained by processing fluorimetric titrations using the Scatchard equation. Cyanines have shown selectivity with respect to the sequence and secondary structure of nucleic acids. The obtained results also indicate a coexistence of several binding modes, in which intercalation is accompanied by binding of dimer or higher-order aggregates inside the polynucleotide grooves.
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:217:889313
ProjectNumber: IP-2018-01-4694
Title: Molekularno prepoznavanje DNA:RNA hibridnih i višelančanih struktura u bioanalitičkim i in vitro sustavima
Acronym: DNARNAHyB-MolBio
Leader: Marijana Radić Stojković
Jurisdiction: Croatia
Funder: HRZZ
Funding stream: IP
Study programmeTitle: Graduate university studies in chemistry; specializations in: Research programme
Course: Research programme
Study programme type: university
Study level: graduate
Academic / professional title: magistar/magistra kemije (magistar/magistra kemije)
Type of resourceText
File originBorn digital
Access conditionsOpen access
Embargo expiration date: 2023-10-22
Terms of useLicence In copyright icon
Created on2021-11-08 11:25:57