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master's thesis
Utjecaj visoke temperature na biodostupnost polifenolnih spojeva i antioksidacijski potencijal klica brokule (Brassica oleracea L. convar. botrytis (L.) Alef)
Zagreb: University of Zagreb, Faculty of Science, 2021. urn:nbn:hr:217:189399
Kolomaz, Mihael
University of Zagreb
Faculty of Science
Department of Biology

Institutional repository: Repository of the Faculty of Science

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Kolomaz, M. (2021). Utjecaj visoke temperature na biodostupnost polifenolnih spojeva i antioksidacijski potencijal klica brokule (Brassica oleracea L. convar. botrytis (L.) Alef) (Master's thesis). Zagreb: University of Zagreb, Faculty of Science. Retrieved from https://urn.nsk.hr/urn:nbn:hr:217:189399

Kolomaz, Mihael. "Utjecaj visoke temperature na biodostupnost polifenolnih spojeva i antioksidacijski potencijal klica brokule (Brassica oleracea L. convar. botrytis (L.) Alef)." Master's thesis, University of Zagreb, Faculty of Science, 2021. https://urn.nsk.hr/urn:nbn:hr:217:189399

Kolomaz, Mihael. "Utjecaj visoke temperature na biodostupnost polifenolnih spojeva i antioksidacijski potencijal klica brokule (Brassica oleracea L. convar. botrytis (L.) Alef)." Master's thesis, University of Zagreb, Faculty of Science, 2021. https://urn.nsk.hr/urn:nbn:hr:217:189399

Kolomaz, M. (2021). 'Utjecaj visoke temperature na biodostupnost polifenolnih spojeva i antioksidacijski potencijal klica brokule (Brassica oleracea L. convar. botrytis (L.) Alef)', Master's thesis, University of Zagreb, Faculty of Science, accessed 18 May 2024, https://urn.nsk.hr/urn:nbn:hr:217:189399

Kolomaz M. Utjecaj visoke temperature na biodostupnost polifenolnih spojeva i antioksidacijski potencijal klica brokule (Brassica oleracea L. convar. botrytis (L.) Alef) [Master's thesis]. Zagreb: University of Zagreb, Faculty of Science; 2021 [cited 2024 May 18] Available at: https://urn.nsk.hr/urn:nbn:hr:217:189399

M. Kolomaz, "Utjecaj visoke temperature na biodostupnost polifenolnih spojeva i antioksidacijski potencijal klica brokule (Brassica oleracea L. convar. botrytis (L.) Alef)", Master's thesis, University of Zagreb, Faculty of Science, Zagreb, 2021. Available at: https://urn.nsk.hr/urn:nbn:hr:217:189399

Metadata
TitleUtjecaj visoke temperature na biodostupnost polifenolnih spojeva i antioksidacijski potencijal klica brokule (Brassica oleracea L. convar. botrytis (L.) Alef)
Title (english)Influence of high temperature on bioavailability of polyphenolic compounds and antioxidant potential of broccoli seedlings (Brassica oleracea L. convar. botrytis (L.) Alef)
AuthorMihael Kolomaz
MentorIvana Šola (mentor)
Committee memberIvana Šola (predsjednik povjerenstva)
Committee memberSofia Ana Blažević (član povjerenstva)
Committee memberMarin Ježić (član povjerenstva)
Committee memberDuje Lisičić (član povjerenstva)
GranterUniversity of Zagreb
Faculty of Science
(Department of Biology)
Zagreb
Defense date and country2021-11-11, Croatia
Scientific / art field,
discipline and subdiscipline		NATURAL SCIENCES
Biology
Abstract
Biljke se toplinskom stresu prilagođavaju promjenama u vlastitom kemijskom sastavu. Kupusnjače su zastupljene u prehrani ljudi i životinja, stoga je važno kako će visoka temperatura (VT) utjecati na njihovu nutritivnu vrijednost. Cilj ovoga rada bio je istražiti utjecaj VT (38°C) na biodostupnost različitih skupina i pojedinačnih polifenolnih spojeva u klicama brokule (Brassica oleracea L. convar. botrytis (L.) Alef.) prije i tijekom simulirane ljudske probave in vitro, kao i na njihov antioksidacijski potencijal, kako bi se utvrdio stupanj stabilnosti/podložnosti navedenih parametara prema VT. Spektrofotometrijski je određen udio različitih skupina spojeva, a metodom HPLC razdvojeni su, identificirani i kvantificirani metaboliti: L-askorbinska, galna, benzojeva, kavena, p-kumarinska, ferulična i sinapinska kiselina te tilirozid, kempferol i kvercetin. Antioksidacijski potencijal određen je metodama ABTS, DPPH i FRAP. VT značajno je povećala biodostupnost ukupnih flavonola i hidroksicimetnih kiselina klica brokule. Jedini spoj čija se koncentracija razlikovala u izvornom ekstraktu između pokusnih skupina je ferulična kiselina, kojoj se koncentracija pri VT snizila, pa bi je se moglo razmotriti kao biomarker VT okoliša. Što se tiče probave, biodostupnost benzojeve kiseline je veća, a kavene manja iz klica uzgojenih pri VT tijekom svih faza probave. Biodostupnost kvercetina u fazama usta i želuca bila je viša kod klica uzgajanih pri VT. VT je smanjila ili nije utjecala na antioksidacijski potencijal klica brokule u izvornom ekstraktu i nakon probave in vitro.
Abstract (english)
Plants adapt to heat stress by changing their own chemical composition. Brassicaceae are present in the diet of humans and animals, so it is important to know how high temperature (HT) will affect their nutritional value. The aim of this study was to investigate the influence of HT (38°C) on the bioavailability of different groups and individual polyphenolic compounds in broccoli sprouts (Brassica oleracea L. convar. botrytis (L.) Alef.) before and after in vitro simulated human digestion, as well as on their antioxidant potential, in order to determine the degree of stability/susceptibility of these parameters to HT. The proportion of different groups of compounds was determined spectrophotometrically, and individual metabolites were separated, identified and quantified by HPLC: L-ascorbic, gallic, benzoic, caffeic, p-coumaric, ferulic and synapic acid, and tyroside, kaempferol and quercetin. Antioxidant potential was determined by ABTS, DPPH and FRAP methods. HT significantly increased the bioavailability of total flavonols and hydroxycinnamic acids of broccoli sprouts. The only compound which concentration differed in the original extract between the experimental groups was ferulic acid; at HT its concentration decreased, so it could be assessed as a potential enviromental HT biomarker.. In terms of digestion, the bioavailability of benzoic acid was higher and caffeine lower from sprouts grown at HT during each phase of digestion. The bioavailability of quercetin in the salivary and gastric phase was higher in sprouts grown at HT. HT reduced or did not affect the antioxidant potential of broccoli sprouts in the original extract and after in vitro digestion.
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:217:189399
Study programmeTitle: Molecular Biology
Study programme type: university
Study level: graduate
Academic / professional title: magistar/magistra molekularne biologije (magistar/magistra molekularne biologije)
Type of resourceText
File originBorn digital
Access conditionsOpen access
Embargo expiration date: 2023-11-11
Terms of useLicence In copyright icon
Created on2021-11-12 13:29:38