prikaz prve stranice dokumenta Promjene genoma u atipičnim stanicama u urinu dokazane metodom fluorescentne hibridizacije in situ
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master's thesis
Promjene genoma u atipičnim stanicama u urinu dokazane metodom fluorescentne hibridizacije in situ
Zagreb: University of Zagreb, Faculty of Science, 2012. urn:nbn:hr:217:201558
Jurković, Sven
University of Zagreb
Faculty of Science
Department of Biology

Institutional repository: Repository of the Faculty of Science

Cite this document

Jurković, S. (2012). Promjene genoma u atipičnim stanicama u urinu dokazane metodom fluorescentne hibridizacije in situ (Master's thesis). Zagreb: University of Zagreb, Faculty of Science. Retrieved from https://urn.nsk.hr/urn:nbn:hr:217:201558

Jurković, Sven. "Promjene genoma u atipičnim stanicama u urinu dokazane metodom fluorescentne hibridizacije in situ." Master's thesis, University of Zagreb, Faculty of Science, 2012. https://urn.nsk.hr/urn:nbn:hr:217:201558

Jurković, Sven. "Promjene genoma u atipičnim stanicama u urinu dokazane metodom fluorescentne hibridizacije in situ." Master's thesis, University of Zagreb, Faculty of Science, 2012. https://urn.nsk.hr/urn:nbn:hr:217:201558

Jurković, S. (2012). 'Promjene genoma u atipičnim stanicama u urinu dokazane metodom fluorescentne hibridizacije in situ', Master's thesis, University of Zagreb, Faculty of Science, accessed 25 November 2024, https://urn.nsk.hr/urn:nbn:hr:217:201558

Jurković S. Promjene genoma u atipičnim stanicama u urinu dokazane metodom fluorescentne hibridizacije in situ [Master's thesis]. Zagreb: University of Zagreb, Faculty of Science; 2012 [cited 2024 November 25] Available at: https://urn.nsk.hr/urn:nbn:hr:217:201558

S. Jurković, "Promjene genoma u atipičnim stanicama u urinu dokazane metodom fluorescentne hibridizacije in situ", Master's thesis, University of Zagreb, Faculty of Science, Zagreb, 2012. Available at: https://urn.nsk.hr/urn:nbn:hr:217:201558

Metadata
TitlePromjene genoma u atipičnim stanicama u urinu dokazane metodom fluorescentne hibridizacije in situ
Title (english)Genome changes in atypical urine cells proved by fluorescence in situ hybridization method
AuthorSven Jurković
MentorInga Marijanović (mentor)
Committee memberInga Marijanović (predsjednik povjerenstva)
Committee memberDubravka Hranilović (član povjerenstva)
Committee memberDamjan Franjević (član povjerenstva)
GranterUniversity of Zagreb
Faculty of Science
(Department of Biology)
Zagreb
Defense date and country2012-02-20, Croatia
Scientific / art field,
discipline and subdiscipline		NATURAL SCIENCES
Biology
Abstract
Karcinom mokraćnog mjehura jedan je od vodećih karcinoma u muškoj populaciji. Visoko osjetljivim, specifičnim i neinvazivnim metodama molekularne biologije kao što je interfazni FISH (fluorescentna hibridizacija in situ) moguće je detektirati promjene genoma u stanicama sedimenta. Cilj je bio ispitati i dokazati prisutnost promjena genoma u atipičnim stanicama sedimenta urina. Tehnikom interfaznog FISH-a detektirane su numeričke i strukturne promjene pomoću četiri obilježene probe koje hibridiziraju za centromerne regije kromosoma 3 (CEP 3), kromosoma 7 (CEP 7) i kromosoma 17 (CEP 17) odnosno za lokus 9p21 (LSI p16). Analizirano je 50 uzoraka urina u bolesnika kod kojih je bila prisutna stanična atipija. Pozitivan nalaz nađen je kod 21 (42%) bolesnika. U pet (24%) bolesnika bile su prisutne numeričke i strukturne promjene, u 15 (71%) samo numeričke, a u jednog pacijenta (5%) samo strukturne promjene. Dakle, tehnikom interfaznog FISH-a mogu se detektirati i pratiti poznate promjene genoma u stanicama tumora mokraćnog mjehura.
Abstract (english)
Bladder cancer is one of the leading carcinomas in male population. It is possible to detect genome changes in urine sediment cells by highly sensitive, specific and noninvasive molecular biology methods like interphase-FISH (fluorescent in situ hybridization). The aim was to examine and prove genome changes in atypic cells in urine. Structural and numeric chromosomal aberrations were detected by interphase-FISH method, which uses four labeled probes which hybridize to centromeric regions of chromosome 3 (CEP 3), chromosome 7 (CEP 7) and chromosome 17 (CEP 17), and 9p21 locus (LSI p16). 50 samples of urine of patients were analysed. Positive result was found in 21 (42%) patients. In 5 (24%) patients there were just numeric, in 15 (71%) patients just structural, and in 1 (5%) patient both structural and numeric chromosomal aberrations. The conclusion is that familiar genome changes in bladder cancer cells can be detected and monitored by interphase-FISH method.
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:217:201558
Study programmeTitle: Molecular Biology
Study programme type: university
Study level: graduate
Academic / professional title: magistar/magistra molekularne biologije (magistar/magistra molekularne biologije)
Type of resourceText
File originBorn digital
Access conditionsAccess restricted to students and staff of home institution
Terms of useLicence In copyright icon
Created on2017-05-02 08:38:25