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master's thesis
Analysis of tau protein spread in rat brain as a model of Alzheimer's disease
Zagreb: University of Zagreb, Faculty of Science, 2021. urn:nbn:hr:217:244721
Horvat Velić, Emina
University of Zagreb
Faculty of Science
Department of Biology

Institutional repository: Repository of the Faculty of Science

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Horvat Velić, E. (2021). Analysis of tau protein spread in rat brain as a model of Alzheimer's disease (Master's thesis). Zagreb: University of Zagreb, Faculty of Science. Retrieved from https://urn.nsk.hr/urn:nbn:hr:217:244721

Horvat Velić, Emina. "Analysis of tau protein spread in rat brain as a model of Alzheimer's disease." Master's thesis, University of Zagreb, Faculty of Science, 2021. https://urn.nsk.hr/urn:nbn:hr:217:244721

Horvat Velić, Emina. "Analysis of tau protein spread in rat brain as a model of Alzheimer's disease." Master's thesis, University of Zagreb, Faculty of Science, 2021. https://urn.nsk.hr/urn:nbn:hr:217:244721

Horvat Velić, E. (2021). 'Analysis of tau protein spread in rat brain as a model of Alzheimer's disease', Master's thesis, University of Zagreb, Faculty of Science, accessed 27 April 2024, https://urn.nsk.hr/urn:nbn:hr:217:244721

Horvat Velić E. Analysis of tau protein spread in rat brain as a model of Alzheimer's disease [Master's thesis]. Zagreb: University of Zagreb, Faculty of Science; 2021 [cited 2024 April 27] Available at: https://urn.nsk.hr/urn:nbn:hr:217:244721

E. Horvat Velić, "Analysis of tau protein spread in rat brain as a model of Alzheimer's disease", Master's thesis, University of Zagreb, Faculty of Science, Zagreb, 2021. Available at: https://urn.nsk.hr/urn:nbn:hr:217:244721

Metadata
TitleAnalysis of tau protein spread in rat brain as a model of Alzheimer's disease
Title (croatian)Analiza širenja proteina tau u mozgu štakora kao modela Alzheimerove bolesti
AuthorEmina Horvat Velić
MentorGoran Šimić (mentor)
MentorDubravka Hranilović (sumentor)
Committee memberDubravka Hranilović (predsjednik povjerenstva)
Committee memberJasna Lajtner (član povjerenstva)
Committee memberMartina Šeruga Musić (član povjerenstva)
Committee memberSofia Ana Blažević (član povjerenstva)
GranterUniversity of Zagreb
Faculty of Science
(Department of Biology)
Zagreb
Defense date and country2021-11-17, Croatia
Scientific / art field,
discipline and subdiscipline		NATURAL SCIENCES
Biology
Abstract
Alzheimer's disease (AD) is a neurodegenerative disease and the most common primary cause of dementia syndrome. Many theories exist regarding possible mechanisms of development and progression of AD, one of which is the tau hypothesis. This theory suggests that the hyperphosphorylated tau proteins misfold, oligomerize and aggregate, disrupting microtubule structure and function ultimately leading to the death of synapses and neurons. In this work we investigated changes and spreading of tau fibrils and tau oligomers that were inoculated into the medial entorhinal cortex of nine Wistar rats. The phosphorylated tau proteins were visualised by using the AT8 antibody, which binds to Ser202/Thr205 epitope of tau. Three coronal levels (-8.04, -6.84, and -5.64 mm from bregma) were analysed at 4, 8, and 11-month post-inoculation. The main finding was that the animals inoculated with the tau fibrils displayed significantly greater amount of neurofibrillary changes and spread of tau fibrils, while such changes did not occur in animals inoculated with tau oligomers and phosphate buffer saline (controls). These findings suggest that tau fibrils may be the true culprit in AD and thus deserve further validation and a more detailed analysis in future studies.
Abstract (croatian)
Alzheimerova bolest (AB) je neurodegenerativna bolest i najčešći uzrok sindroma demencije. Postoje mnoge teorije o mogućim mehanizmima nastanka i širenja AB-a, od kojih je jedna tau hipoteza. Ta teorija pretpostavlja da hiperfosforilacija tau proteina dovodi do njihovog pogrešnog smatanja, oligomerizacije i nakupljanja, što remeti strukturu i funkciju mikrotubula te u konačnici odumiranja sinapsi i neurona. U ovom radu smo istražili promjene i širenje tau fibrila i tau oligomera inokuliranih u medijalni dio entorinalne moždane kore devet Wistar štakora. Za imunohistokemijsku vizualizaciju fosforiliranog tau proteina na epitopu Ser202/Thr205 korišteno je AT8 protutijelo. Tri koronalne razine (udaljenost od bregme -8,04, -6,84 i -5,64 mm) analizirane su 4, 8 i 11 mjeseci nakon inokulacije. Glavni nalaz je bio taj da su u životinjama kojima su inokulirane tau fibrile uočene značajne neurofibrilarne promjene i širenje tau fibrila, dok se takve promjene nisu dogodile kod životinja kojima su inokulirani tau oligomeri ili otopina fosfatnog pufera (kontrole). Navedeni rezultati upućuju da bi tau fibrile mogle biti uzročnik nastanka AB-a, pa stoga zaslužuju daljnju validaciju i detaljniju analizu u budućim istraživanjima.
Keywords
Keywords (english)
Languageenglish
URN:NBNurn:nbn:hr:217:244721
Study programmeTitle: Molecular Biology
Study programme type: university
Study level: graduate
Academic / professional title: magistar/magistra molekularne biologije (magistar/magistra molekularne biologije)
Type of resourceText
File originBorn digital
Access conditionsOpen access
Embargo expiration date: 2023-11-17
Terms of useLicence In copyright icon
Created on2021-11-22 13:59:32