The Burkholderia cepacia (BCC) bacterial complex is a group of gram-negative nonfermentative bacteria. They are present in the natural environment, but also appear as an opportunistic pathogen in immunocompromised patients. The subject of this research was to determine the possibility of their survival and eventual multiplication in autoclaved commercial spring water poor and rich in nutrients. Four environmental BCC isolates previously isolated from wild landfill, stream sediment near the town of Zabok, Krapina river water, agricultural soil fertilized with poultry manure and one clinical BCC isolate were used for the research. The individual BCC isolates were suspended in nutrient-poor and nutrient-rich water and incubated at 22 °C for 50 days. The number of viable BCC isolates was counted at the beginning of the experiment and at certain time intervals, and the survival rate of each isolate was calculated. The results showed that in water with the addition of nutrients, all isolates successfully survived and multiplied. In nutrient-poor water, the multiplication of isolates was weaker, but they survived better than nutrient-rich water. Since BCC bacteria can survive and multiply in water for a long time, the environment should be considered the main source of these pathogens.