prikaz prve stranice dokumenta Notch i Ikaros u diferencijaciji hematopoetskih stanica
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master's thesis
Notch i Ikaros u diferencijaciji hematopoetskih stanica
Zagreb: University of Zagreb, Faculty of Science, 2012. urn:nbn:hr:217:434380
Konjević, Matea
University of Zagreb
Faculty of Science
Department of Biology

Institutional repository: Repository of the Faculty of Science

Cite this document

Konjević, M. (2012). Notch i Ikaros u diferencijaciji hematopoetskih stanica (Master's thesis). Zagreb: University of Zagreb, Faculty of Science. Retrieved from https://urn.nsk.hr/urn:nbn:hr:217:434380

Konjević, Matea. "Notch i Ikaros u diferencijaciji hematopoetskih stanica." Master's thesis, University of Zagreb, Faculty of Science, 2012. https://urn.nsk.hr/urn:nbn:hr:217:434380

Konjević, Matea. "Notch i Ikaros u diferencijaciji hematopoetskih stanica." Master's thesis, University of Zagreb, Faculty of Science, 2012. https://urn.nsk.hr/urn:nbn:hr:217:434380

Konjević, M. (2012). 'Notch i Ikaros u diferencijaciji hematopoetskih stanica', Master's thesis, University of Zagreb, Faculty of Science, accessed 23 December 2024, https://urn.nsk.hr/urn:nbn:hr:217:434380

Konjević M. Notch i Ikaros u diferencijaciji hematopoetskih stanica [Master's thesis]. Zagreb: University of Zagreb, Faculty of Science; 2012 [cited 2024 December 23] Available at: https://urn.nsk.hr/urn:nbn:hr:217:434380

M. Konjević, "Notch i Ikaros u diferencijaciji hematopoetskih stanica", Master's thesis, University of Zagreb, Faculty of Science, Zagreb, 2012. Available at: https://urn.nsk.hr/urn:nbn:hr:217:434380

Metadata
TitleNotch i Ikaros u diferencijaciji hematopoetskih stanica
Title (english)Notch and Ikaros in hematopoietic cell differentiation
AuthorMatea Konjević
MentorMariastefania Antica (mentor)
MentorMaja Matulić (mentor)
Committee memberMaja Matulić (predsjednik povjerenstva)
Committee memberMariastefania Antica (član povjerenstva)
Committee memberNada Oršolić (član povjerenstva)
GranterUniversity of Zagreb
Faculty of Science
(Department of Biology)
Zagreb
Defense date and country2012-02-14, Croatia
Scientific / art field,
discipline and subdiscipline		NATURAL SCIENCES
Biology
Abstract
Hematopoeza je proces nastajanja novih krvnih stanica. Transkripcijski faktori iz porodice Ikaros i puta dojavljivanja Notch reguliraju važne kontrolne točke tijekom hematopoeze koje će odlučiti u kojem će se smjeru stanica diferencirati. U radu je potvrđena i izmjerena ekspresija transkripcijskih faktora iz porodice Ikaros: Ikaros, Helios i Aiolos te puta dojavljivanja Notch: Notch1 i Hes1 u NB4 stanicama prije i poslije diferencijacije ATRA-om. U radu je uspješno uhodan, prilagođen i primijenjen protokol za metodu višestruke lančane reakcije polimerazom (multiplex PCR). Metodom je istovremeno umnoženo 6 odsječaka DNA u uzorku od 2500 stanica. Za multiplex PCR su odabrane početnice te provjerene i testirane na specifičnost i izbjegavanje sekundarnih struktura. Semi-kvantitativni RT-PCR je korišten u svrhu otkrivanja moguće promjene u razinama mRNA u stanicama prije i poslije diferencijacije. Smanjenje u ekspresiji gena Aiolos i Hes1 ukazuje na njihovu moguću ulogu u diferencijaciji i razvoju nezrelih mijeloidnih stanica.
Abstract (english)
Hematopoiesis is the process of formation of new blood cells. Transcription factors from the Ikaros family and Notch signaling pathway regulate important checkpoints during hematopoiesis, which will decide in which direction the cells differentiate. In this work was confirmed and measured expression of transcription factors from the Ikaros family: Ikaros, Helios and Aiolos and Notch signaling pathway: Notch1 and Hes1 in NB4 cells before and after differentiation induced with ATRA. Protocol for the method of multiplex polymerase chain reaction (multiplex PCR) was successfully established, adapted and implemented. Six segments of DNA was simultaneously amplified in a sample of 2500 cells. For multiplex PCR, primers were selected and tested for specificity and to avoid secondary structures. Semi-quantitative RT-PCR was used to detect possible changes in the levels of mRNA in cells before and after differentiation. The reduction in expression of genes Aiolos and Hes1 suggests their possible role in differentiation and development of immature myeloid cells.
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:217:434380
Study programmeTitle: Experimental Biology
Study programme type: university
Study level: graduate
Academic / professional title: magistar/magistra eksperimentalne biologije (magistar/magistra eksperimentalne biologije)
Type of resourceText
File originBorn digital
Access conditionsAccess restricted to students and staff of home institution
Terms of useLicence In copyright icon
Created on2017-05-02 08:47:56