prikaz prve stranice dokumenta Molekularno-filogenetski odnosi unutar roda Rana (Amphibia, Ranidae)na osnovi metode RFLP i analize sekvenci za 16S, cytb i ITS2
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master's thesis
Molekularno-filogenetski odnosi unutar roda Rana (Amphibia, Ranidae)na osnovi metode RFLP i analize sekvenci za 16S, cytb i ITS2
Zagreb: University of Zagreb, Faculty of Science, 2012. urn:nbn:hr:217:083543
Korlević, Petra
University of Zagreb
Faculty of Science
Department of Biology

Institutional repository: Repository of the Faculty of Science

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Korlević, P. (2012). Molekularno-filogenetski odnosi unutar roda Rana (Amphibia, Ranidae)na osnovi metode RFLP i analize sekvenci za 16S, cytb i ITS2 (Master's thesis). Zagreb: University of Zagreb, Faculty of Science. Retrieved from https://urn.nsk.hr/urn:nbn:hr:217:083543

Korlević, Petra. "Molekularno-filogenetski odnosi unutar roda Rana (Amphibia, Ranidae)na osnovi metode RFLP i analize sekvenci za 16S, cytb i ITS2." Master's thesis, University of Zagreb, Faculty of Science, 2012. https://urn.nsk.hr/urn:nbn:hr:217:083543

Korlević, Petra. "Molekularno-filogenetski odnosi unutar roda Rana (Amphibia, Ranidae)na osnovi metode RFLP i analize sekvenci za 16S, cytb i ITS2." Master's thesis, University of Zagreb, Faculty of Science, 2012. https://urn.nsk.hr/urn:nbn:hr:217:083543

Korlević, P. (2012). 'Molekularno-filogenetski odnosi unutar roda Rana (Amphibia, Ranidae)na osnovi metode RFLP i analize sekvenci za 16S, cytb i ITS2', Master's thesis, University of Zagreb, Faculty of Science, accessed 27 December 2024, https://urn.nsk.hr/urn:nbn:hr:217:083543

Korlević P. Molekularno-filogenetski odnosi unutar roda Rana (Amphibia, Ranidae)na osnovi metode RFLP i analize sekvenci za 16S, cytb i ITS2 [Master's thesis]. Zagreb: University of Zagreb, Faculty of Science; 2012 [cited 2024 December 27] Available at: https://urn.nsk.hr/urn:nbn:hr:217:083543

P. Korlević, "Molekularno-filogenetski odnosi unutar roda Rana (Amphibia, Ranidae)na osnovi metode RFLP i analize sekvenci za 16S, cytb i ITS2", Master's thesis, University of Zagreb, Faculty of Science, Zagreb, 2012. Available at: https://urn.nsk.hr/urn:nbn:hr:217:083543

Metadata
TitleMolekularno-filogenetski odnosi unutar roda Rana (Amphibia, Ranidae)na osnovi metode RFLP i analize sekvenci za 16S, cytb i ITS2
Title (english)Phylogenetic relationships within the genus Rana (Amphibia, Ranidae) based on a method RFLP and 16S, cytb, ITS2 sequence analysis
AuthorPetra Korlević
MentorDamjan Franjević (mentor)
Committee memberDamjan Franjević (predsjednik povjerenstva)
Committee memberDomagoj Đikić (član povjerenstva)
Committee memberKristian Vlahoviček (član povjerenstva)
GranterUniversity of Zagreb
Faculty of Science
(Department of Biology)
Zagreb
Defense date and country2012-02-24, Croatia
Scientific / art field,
discipline and subdiscipline		NATURAL SCIENCES
Biology
Abstract
Proteini MAB sadrţe dvije funkcionalno bitne interakcijske domene: MATH i BTB. Do sada je pokazano da su oni dio sustava eliminacije proteina koji stanici više nisu potrebni. Neki od istraženih proteina MAB (CeMEL-26, HsSPOP) dio su multiproteinskih kompleksa kulin 3 E3 ligaza i sluţe za prepoznavanje supstrata koje treba razgraditi. Tijekom razvoja pšenice ekspresija gena TaMAB2 prisutna je samo u oplođenoj jajnoj stanici, u zigoti te u dvostaničnom embriju. U ovom radu napravljena je heterologna ekspresija gena TaMAB2 iz pšenice u uročnjaku. Utvrđeno je da transgenične biljke proizvode cjeloviti protein TaMAB2 koji se u stanicama nakuplja. Sam protein TaMAB2 je ubikvitiniran, te vjerojatno stvara homo- i heterodimere. Rast i razvoj transgeničnih biljaka je značajno fenotipski promijenjen u odnosu na biljke divljeg tipa ali im fototropizam i gravitropizam nisu poremećeni. Analizom unutarstanične lokalizacije proteina TaMAB2 u stanicama BY-2 i pokožici luka utvrđeno je nakupljanje velikih agregata oko jezgrine ovojnice i u blizini stanične membrane. Ovi rezultati ukazuju da bi protein TaMAB2 mogao imati bitnu ulogu u eliminaciji proteina i uspostavljanju polarnosti tijekom rane embriogeneze pšenice.
Abstract (english)
Water frogs (genus Rana, subgenus Pelophylax) present an interesting research subject for ecology, physiology and genetics. Rana kl. esculenta hybridogenetic complex, which consists of two parental species (Rana ridibunda and Rana lessonae) and their hybrid species (Rana kl. esculenta), is of special interest. Hybrids often show morphological traits similar to either parent, making them harder to identify on the field. Because of that, more relevance is given to molecular and phylogenetic methods in the identification of water frog species. Identification by morphological traits has shown that in central Croatia both parental populations of R. ridibunda and R. lessonae can inhabit the same location and thus lead to the formation of hybrids. The goal of this project was to determine the phylogenetic relationships of water frog populations from Crna Mlaka based on 16S rRNA, cytochrome b and ITS2sequence analysis, and to examine the efficiency of a simple RFLP method for identifying parental species and hybrids based on the digestion of a conserved ITS2 fragment with three restriction enzymes (KpnI, HaeII and SmaI). Results of this phylogenetic analysis have shown that both parental species R. ridibunda and R. lessonae, and thus potential hybrids, inhabit Crna Mlaka. Phylogenetic analysis of mitochondrial 16S rRNA and cytb genes proved to be the best method for species and haplotype identification, while the RFLP method and phylogenetic analysis of nuclear ITS2 regions, which could help differentiate hybrid samples, need further protocol optimization.
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:217:083543
Study programmeTitle: Molecular Biology
Study programme type: university
Study level: graduate
Academic / professional title: magistar/magistra molekularne biologije (magistar/magistra molekularne biologije)
Type of resourceText
File originBorn digital
Access conditionsAccess restricted to students and staff of home institution
Terms of useLicence In copyright icon
Created on2017-05-02 08:52:15