Astrocytomas are the most common type of glioma. They are classified into three malignancy grades: diffuse astrocytoma, anaplastic astrocytoma, and glioblastoma. The Wnt signaling pathway is essential in many biological processes, so its aberrant activity has an important role in tumorigenesis. The Secreted Frizzled-Related Protein (SFRP) family modulates Wnt signaling. It has been shown in various tumors that these genes are frequently hypermethylated, and thereby transcriptionally silent, eventually leading to uncontrolled transcription of oncogenes. The aim of this study was to determine whether there is a different frequency of alterations in the methylation status and the level of SFRP4 protein expression between different histological grades of astrocytomas. SFRP4 protein expression was detected by immunohistochemistry, while promoter methylation of the same samples was examined by MS PCR. Statistical analysis revealed that increase in astrocytoma grade results in SFRP4 protein expression decrease (p=0.008), indicating its role as a tumor suppressor by inhibiting Wnt signaling pathway activity and preventing tumor formation and progression. Methylation analysis revealed that 72.7% SFRP4 promoters in diffuse astrocytoma were methylated, while in higher tumor grades, methylated promoters were not detected. A statistically significant difference in the methylation status of the SFRP4 gene was found between malignancy grades of astrocytic tumors (p<0.001). These findings suggest that some other epigenetic mechanisms regulate SFRP4 expression in higher astrocytoma grades.