prikaz prve stranice dokumenta Inhibicija urokiaznog aktivacijskog sustava plazminogena u stanicama raka dojke
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master's thesis
Inhibicija urokiaznog aktivacijskog sustava plazminogena u stanicama raka dojke
Zagreb: University of Zagreb, Faculty of Science, 2012. urn:nbn:hr:217:907247
Majstorović, Ivana
University of Zagreb
Faculty of Science
Department of Biology

Institutional repository: Repository of the Faculty of Science

Cite this document

Majstorović, I. (2012). Inhibicija urokiaznog aktivacijskog sustava plazminogena u stanicama raka dojke (Master's thesis). Zagreb: University of Zagreb, Faculty of Science. Retrieved from https://urn.nsk.hr/urn:nbn:hr:217:907247

Majstorović, Ivana. "Inhibicija urokiaznog aktivacijskog sustava plazminogena u stanicama raka dojke." Master's thesis, University of Zagreb, Faculty of Science, 2012. https://urn.nsk.hr/urn:nbn:hr:217:907247

Majstorović, Ivana. "Inhibicija urokiaznog aktivacijskog sustava plazminogena u stanicama raka dojke." Master's thesis, University of Zagreb, Faculty of Science, 2012. https://urn.nsk.hr/urn:nbn:hr:217:907247

Majstorović, I. (2012). 'Inhibicija urokiaznog aktivacijskog sustava plazminogena u stanicama raka dojke', Master's thesis, University of Zagreb, Faculty of Science, accessed 04 November 2024, https://urn.nsk.hr/urn:nbn:hr:217:907247

Majstorović I. Inhibicija urokiaznog aktivacijskog sustava plazminogena u stanicama raka dojke [Master's thesis]. Zagreb: University of Zagreb, Faculty of Science; 2012 [cited 2024 November 04] Available at: https://urn.nsk.hr/urn:nbn:hr:217:907247

I. Majstorović, "Inhibicija urokiaznog aktivacijskog sustava plazminogena u stanicama raka dojke", Master's thesis, University of Zagreb, Faculty of Science, Zagreb, 2012. Available at: https://urn.nsk.hr/urn:nbn:hr:217:907247

Metadata
TitleInhibicija urokiaznog aktivacijskog sustava plazminogena u stanicama raka dojke
Title (english)Inhibition of Urokinase Plasminogen Activation System in Breast Cancer Cells
AuthorIvana Majstorović
MentorMaja Matulić (mentor)
GranterUniversity of Zagreb
Faculty of Science
(Department of Biology)
Zagreb
Defense date and country2012-07-02, Croatia
Scientific / art field,
discipline and subdiscipline		NATURAL SCIENCES
Biology
Abstract
Urokinazni aktivacijski sustav plazminogena je više komponentni regulatorni sustav koji ima ključnu ulogu u mnogim fiziološkim i patološkim procesima, uglavnom putem vanstanične proteolize. Sačinjen je od urokinaznog aktivatora plazminogena, njegovog receptora i dva inhibitora. Cilj ovog diplomskog je bio istražiti učinke natrijevog salicilata, aktivnog metabolita aspirina, na morfologiju i bazalnu aktivnost urokinaze kod MDA MB-231 stanica. Zimografija i kazeinoliza su pokazale smanjenje aktivnosti urokinaze kod stanica tretiranih s natrijevim salicilatom ovisno o koncentraciji i duljini inkubacije. Dok je RT PCR nije pokazao značajne razlike u razini transkripcije urokinaze, njenog receptora i inhibitora, Western blot je pokazao blago smanjenje urokinazne ekspresije. Analiza proteinskih ekstrakata pokazala je malu promjenu u razini NF-κB ekspresije, a RT PCR je pokazao smanjnje mRNA integrina αv and β5. Zaključeno je da natrijev salicilat izaziva smanjenje u aktivnosti urokinaze i ekspresije proteina.
Abstract (english)
Urokinase plasminogen activation system is a multi-component regulatory system that plays a key role in many physiological and pathological processes, mainly by extracellular proteolysis. It consists of urokinase plasminogen activator, its receptor and two inhibitors. The aim of this thesis was to investigate the effects of sodium salicylate, an active aspirin metabolite, on MDA MB-231 morphology and basal urokinase plasminogen activator activity. Zymography and caseinolysis following cell treatment with salicylate revealed a concentration and time dependant decrease in urokinase activity. While RT PCR revealed no significant changes in transcription levels of urokinase, its receptor, and inhibitor, Western blot showed a mild decrease in urokinase expression. Analysis of protein extracts indicated a slight change of NF-κB expression, and RT PCR showed decrease in integrin αv and β5 mRNA. It was concluded that sodium salicylate caused decrease in uPA activity and integrin expression.
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:217:907247
Study programmeTitle: Molecular Biology
Study programme type: university
Study level: graduate
Academic / professional title: magistar/magistra molekularne biologije (magistar/magistra molekularne biologije)
Type of resourceText
File originBorn digital
Access conditionsAccess restricted to students and staff of home institution
Terms of useLicence In copyright icon
Created on2017-05-02 09:29:13