prikaz prve stranice dokumenta Homologna i heterologna ekspresija gena AtBPM1 u transgeničnim biljkama Arabidopsis thaliana i transformiranim stanicama Nicotiana tabacum
Access restricted to students and staff of home institution
master's thesis
Homologna i heterologna ekspresija gena AtBPM1 u transgeničnim biljkama Arabidopsis thaliana i transformiranim stanicama Nicotiana tabacum
Zagreb: University of Zagreb, Faculty of Science, 2012. urn:nbn:hr:217:012699
Martinčić, Jelena
University of Zagreb
Faculty of Science
Department of Biology

Institutional repository: Repository of the Faculty of Science

Cite this document

Martinčić, J. (2012). Homologna i heterologna ekspresija gena AtBPM1 u transgeničnim biljkama Arabidopsis thaliana i transformiranim stanicama Nicotiana tabacum (Master's thesis). Zagreb: University of Zagreb, Faculty of Science. Retrieved from https://urn.nsk.hr/urn:nbn:hr:217:012699

Martinčić, Jelena. "Homologna i heterologna ekspresija gena AtBPM1 u transgeničnim biljkama Arabidopsis thaliana i transformiranim stanicama Nicotiana tabacum." Master's thesis, University of Zagreb, Faculty of Science, 2012. https://urn.nsk.hr/urn:nbn:hr:217:012699

Martinčić, Jelena. "Homologna i heterologna ekspresija gena AtBPM1 u transgeničnim biljkama Arabidopsis thaliana i transformiranim stanicama Nicotiana tabacum." Master's thesis, University of Zagreb, Faculty of Science, 2012. https://urn.nsk.hr/urn:nbn:hr:217:012699

Martinčić, J. (2012). 'Homologna i heterologna ekspresija gena AtBPM1 u transgeničnim biljkama Arabidopsis thaliana i transformiranim stanicama Nicotiana tabacum', Master's thesis, University of Zagreb, Faculty of Science, accessed 22 November 2024, https://urn.nsk.hr/urn:nbn:hr:217:012699

Martinčić J. Homologna i heterologna ekspresija gena AtBPM1 u transgeničnim biljkama Arabidopsis thaliana i transformiranim stanicama Nicotiana tabacum [Master's thesis]. Zagreb: University of Zagreb, Faculty of Science; 2012 [cited 2024 November 22] Available at: https://urn.nsk.hr/urn:nbn:hr:217:012699

J. Martinčić, "Homologna i heterologna ekspresija gena AtBPM1 u transgeničnim biljkama Arabidopsis thaliana i transformiranim stanicama Nicotiana tabacum", Master's thesis, University of Zagreb, Faculty of Science, Zagreb, 2012. Available at: https://urn.nsk.hr/urn:nbn:hr:217:012699

Metadata
TitleHomologna i heterologna ekspresija gena AtBPM1 u transgeničnim biljkama Arabidopsis thaliana i transformiranim stanicama Nicotiana tabacum
Title (english)Homologous and heterologous expression of AtBPM1 in Arabidopsis thaliana plants and in transformed Nicotiana tabacum cells
AuthorJelena Martinčić
MentorNataša Bauer (mentor)
Committee memberNataša Bauer (predsjednik povjerenstva)
Committee memberMirta Tkalec (član povjerenstva)
Committee memberMirjana Kalafatić (član povjerenstva)
GranterUniversity of Zagreb
Faculty of Science
(Department of Biology)
Zagreb
Defense date and country2012-02-03, Croatia
Scientific / art field,
discipline and subdiscipline		NATURAL SCIENCES
Biology
Abstract
Proteini s proteinskim domenama BTB/POZ su identificirani u mnogih eukariota gdje su im definirane raznolike funkcije. Takvi proteini mogu sadržavati i dodatne domene, kao što je domena MATH. Domene MATH/BTB (BPM) proteinima biljaka Arabidopsis thaliana omogućuju različite proteinske interakcije. Pretpostavlja se da protein AtBPM1 sudjeluje u prepoznavanju supstrata i njegovoj ubikvitinaciji u sklopu ubikvitin ligaza E3 koje sadrže kulin 3 (CUL3). Točna funkcija proteina AtBPM1 nije definirana. Kako bi pridonijeli razumijevanju funkcije proteina AtBPM1, u ovom radu je istražena njegova lokalizacija u transgeničnim biljkama uročnjaka te u transformiranim stanicama duhana BY-2. Kotransformacijom stanica BY-2 metodom Biolistic® utvrđeno je da proteini AtBPM1 i ZmCUL3 kolokaliziraju u citoplazmi, a manjim dijelom u jezgri. Protein AtBPM1 lokalizira najvećim dijelom u jezgrici gdje mu je funkcija najvjerojatnije neovisna o CUL3. Nije utvrđeno postojanje stabilnih homodimera i heterodimera proteina AtBPM1, kao ni ubikvitinacija navedenog proteina. Tretman inhibitorom proteasoma MG132 rezultirao je nakupljanjem proteina AtBPM1 i ZmCUL3, što ukazuje na razgradnju navedenih proteina proteasomima.
Abstract (english)
BTB/POZ proteins are identified in many eukaryotes in which they obtain different functions. Such proteins can have some additional domains, like MATH domain. Those domains enable Arabidopsis thaliana MATH/BTB (BPM) proteins to have different protein-protein interactions. It is assumed that AtBPM1 protein participates in substrate recognition and ubiquitination in CUL3 E3 ubiquitin ligases. The exact function of AtBPM1 protein is not defined. In order to contribute to the knowledge of AtBPM1 protein function, the aim of this study was to investigate its localization in transgenic A. thaliana plants and in transformed tobacco BY-2 cells. Due to Biolistic® transformation of BY-2 cells, it is determined that AtBPM1 and ZmCUL3 proteins co-localize in cytoplasm, and partly in nucleus. AtBPM1 protein localizes to a large extent in nucleolus, where its function is probably independent of CUL3. Stable homodimers or heterodimers and ubiquitinated AtBPM1 protein is not found. Treatment with proteasome inhibitor MG132 lead to accumulation of AtBPM1 and ZmCUL3, what indicates on involvement of proteasome in degradation of examined proteins.
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:217:012699
Study programmeTitle: Molecular Biology
Study programme type: university
Study level: graduate
Academic / professional title: magistar/magistra molekularne biologije (magistar/magistra molekularne biologije)
Type of resourceText
File originBorn digital
Access conditionsAccess restricted to students and staff of home institution
Terms of useLicence In copyright icon
Created on2017-05-02 09:36:57