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master's thesis
Priprema i karakterizacija vezikula plazmatske membrane iz stanica alga
Zagreb: University of Zagreb, Faculty of Science, 2022. urn:nbn:hr:217:852292
Balen, Tomo
University of Zagreb
Faculty of Science
Department of Chemistry

Institutional repository: Repository of the Faculty of Science

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Balen, T. (2022). Priprema i karakterizacija vezikula plazmatske membrane iz stanica alga (Master's thesis). Zagreb: University of Zagreb, Faculty of Science. Retrieved from https://urn.nsk.hr/urn:nbn:hr:217:852292

Balen, Tomo. "Priprema i karakterizacija vezikula plazmatske membrane iz stanica alga." Master's thesis, University of Zagreb, Faculty of Science, 2022. https://urn.nsk.hr/urn:nbn:hr:217:852292

Balen, Tomo. "Priprema i karakterizacija vezikula plazmatske membrane iz stanica alga." Master's thesis, University of Zagreb, Faculty of Science, 2022. https://urn.nsk.hr/urn:nbn:hr:217:852292

Balen, T. (2022). 'Priprema i karakterizacija vezikula plazmatske membrane iz stanica alga', Master's thesis, University of Zagreb, Faculty of Science, accessed 22 November 2024, https://urn.nsk.hr/urn:nbn:hr:217:852292

Balen T. Priprema i karakterizacija vezikula plazmatske membrane iz stanica alga [Master's thesis]. Zagreb: University of Zagreb, Faculty of Science; 2022 [cited 2024 November 22] Available at: https://urn.nsk.hr/urn:nbn:hr:217:852292

T. Balen, "Priprema i karakterizacija vezikula plazmatske membrane iz stanica alga", Master's thesis, University of Zagreb, Faculty of Science, Zagreb, 2022. Available at: https://urn.nsk.hr/urn:nbn:hr:217:852292

Metadata
TitlePriprema i karakterizacija vezikula plazmatske membrane iz stanica alga
Title (english)Preparation and characterization of algal plasma membrane vesicles
AuthorTomo Balen
MentorNadica Ivošević DeNardis (mentor)
Committee memberIva Juranović Cindrić (predsjednik povjerenstva)
Committee memberĐani Škalamera (član povjerenstva)
Committee memberJana Pisk (član povjerenstva)
Committee memberSanda Rončević (član povjerenstva)
GranterUniversity of Zagreb
Faculty of Science
(Department of Chemistry)
Zagreb
Defense date and country2022-02-25, Croatia
Scientific / art field,
discipline and subdiscipline		NATURAL SCIENCES
Chemistry
Abstract
U ovom je radu provedena priprema i karakterizacija vezikula plazmatskih membrana dobivenih iz mikroalga Dunaliella tertiolecta uzgojenih kod dvaju saliniteta. Osmotskim šokom mekanih stanica nastale su mikrometarske vezikule koje su karakterizirane metodama oslikavanja primjenom epifluorescencijske, konfokalne i mikroskopije atomskih sila. Pripremljene vezikule bile su mikrometarske, pojedinačne, uglavnom sferičnoga oblika, prazne ili s nešto staničnoga materijala te su pokazale svojstvo autofluorescencije uslijed prisutnosti klorofila koji je ugrađen tijekom rekonstrukcije membrane. Oslikavanjem na nanometarskoj skali vizualizirane su površinske globularne strukture koje bi mogle odgovarati glikoproteinima između kojih su nanometarske pore. Vezikule plazma membrane su propusne za male hidrofilne molekule kalceina, dok su polupropusne za veće molekule konkanavalin A-FITC uz specifičnu interakciju s površinskim skupinama glukozil i manozil na membrani. Rezultati nisu pokazali značajnu razliku u smislu morfologije i propusnosti između vezikula plazma membrana kod dva saliniteta. Rezultati ovoga rada doprinose daljnjem razvoju nove generacije alternativnih bioinspiriranih nosača aktivnih komponenti.
Abstract (english)
In this Thesis plasma membrane vesicles of Dunaliella tertiolecta microalgae grown at two salinities were prepared and characterized. Osmotic shock of soft cells resulted in micrometersized vesicles that were characterized by imaging methods using fluorescence, confocal, and atomic force microscopy. The prepared vesicles were micrometer-sized, single, mostly spherical, empty or with some cellular material, and exhibited the property of autofluorescence due to the presence of chlorophyll incorporated during membrane reconstruction. Nanometerscale measurements show spherical surface structures that could correspond to glycoproteins, with nanometer pores in between. Plasma membrane vesicles are permeable to small hydrophilic calcein molecules, whereas they are semipermeable to larger concavalin A-FITC molecules consistent with specific interaction with glucosyl and mannosyl groups on the membrane. The results showed no significant difference in morphology and permeability between plasma membrane vesicles in the two salinities. The results of this work contribute to the further development of a new generation of alternative bioinspired drug carriers.
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:217:852292
ProjectNumber: IP-2018-01-5840
Title: Od površinskih svojstava stanica alga do pokazatelja stresa u vodenim ekosustavima
Acronym: CELLSTRESS
Leader: Nadica Ivošević DeNardis
Jurisdiction: Croatia
Funder: HRZZ
Funding stream: IP
Study programmeTitle: Graduate university studies in chemistry; specializations in: Research programme
Course: Research programme
Study programme type: university
Study level: graduate
Academic / professional title: magistar/magistra kemije (magistar/magistra kemije)
Type of resourceText
File originBorn digital
Access conditionsOpen access
Embargo expiration date: 2024-02-25
Terms of useLicence In copyright icon
Created on2022-03-02 13:28:56