prikaz prve stranice dokumenta Testing of candidate genes for compound biosynthesis in Arnica montana
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master's thesis
Testing of candidate genes for compound biosynthesis in Arnica montana
Zagreb: University of Zagreb, Faculty of Science, 2022. urn:nbn:hr:217:279243
Mrakovčić, Nikolina
University of Zagreb
Faculty of Science
Department of Biology

Institutional repository: Repository of the Faculty of Science

Cite this document

Mrakovčić, N. (2022). Testing of candidate genes for compound biosynthesis in Arnica montana (Master's thesis). Zagreb: University of Zagreb, Faculty of Science. Retrieved from https://urn.nsk.hr/urn:nbn:hr:217:279243

Mrakovčić, Nikolina. "Testing of candidate genes for compound biosynthesis in Arnica montana." Master's thesis, University of Zagreb, Faculty of Science, 2022. https://urn.nsk.hr/urn:nbn:hr:217:279243

Mrakovčić, Nikolina. "Testing of candidate genes for compound biosynthesis in Arnica montana." Master's thesis, University of Zagreb, Faculty of Science, 2022. https://urn.nsk.hr/urn:nbn:hr:217:279243

Mrakovčić, N. (2022). 'Testing of candidate genes for compound biosynthesis in Arnica montana', Master's thesis, University of Zagreb, Faculty of Science, accessed 14 May 2024, https://urn.nsk.hr/urn:nbn:hr:217:279243

Mrakovčić N. Testing of candidate genes for compound biosynthesis in Arnica montana [Master's thesis]. Zagreb: University of Zagreb, Faculty of Science; 2022 [cited 2024 May 14] Available at: https://urn.nsk.hr/urn:nbn:hr:217:279243

N. Mrakovčić, "Testing of candidate genes for compound biosynthesis in Arnica montana", Master's thesis, University of Zagreb, Faculty of Science, Zagreb, 2022. Available at: https://urn.nsk.hr/urn:nbn:hr:217:279243

Metadata
TitleTesting of candidate genes for compound biosynthesis in Arnica montana
Title (croatian)Testiranje gena kandidata uključenih u biosintezu metabolita biljke Arnica montana
AuthorNikolina Mrakovčić
MentorGundula Noll (mentor)
MentorNataša Bauer (sumentor)
Committee memberNataša Bauer (predsjednik povjerenstva)
Committee memberMirta Tkalec (član povjerenstva)
Committee memberBiljana Balen (član povjerenstva)
Committee memberMarin Ježić (član povjerenstva)
GranterUniversity of Zagreb
Faculty of Science
(Department of Biology)
Zagreb
Defense date and country2022-02-28, Croatia
Scientific / art field,
discipline and subdiscipline		NATURAL SCIENCES
Biology
Abstract
Arnica montana L. is a traditional medicinal plant used for centuries. It owes its medicinal properties to sesquiterpene lactones, helenalin and dihydrohelenalin. Traditionally, the plants are harvested from its natural habitat, but there is need for alternative ways to sustainably obtain bioactive compounds. To achieve alternative production of A. montana bioactive sesquiterpene lactones, their biosynthesis pathway must be elucidated. Functional characterization of candidate genes, that might be involved in sesquiterpene lactones biosynthesis, has been conducted in the heterologous expression system yeast Saccharomyces cerevisiae and compounds analysis via gas chromatography-mass spectrometry. Additionally, analysis of potential candidate genes expression patterns and sesquiterpene lactones content distribution in plants was carried out. Candidate genes AmGAS and AmGAO were verified to encode respective germacrene A synthase and germacrene A acid oxidase, and their enzymatic activity was confirmed. Candidate genes AmCYPs were functionally analysed to a certain extent, with candidate AmCYP7 showing great potential for further investigation. Correlation of expression patterns and content of bioactive compounds in A. montana tissues revealed that among tested, AmCYP7 gene has potential to be part of sesquiterpene lactones biosynthesis pathway and provided preliminary evidence for their biosynthesis at different developmental stages during the plant´s life cycle.
Abstract (croatian)
Arnica montana L. je biljka koja se stoljećima koristi u tradicionalnoj medicini. Za njezina ljekovita svojstva odgovorni su seskviterpenski laktoni, helenalin i dihidrohelenalin. Arnika se tradicionalno ubire s prirodnog staništa i postoji potreba za alternativnim i održivijim načinima sinteze bioaktivnih sastojaka. Kako bi alternativna proizvodnja bioaktivnih seskviterpenskih laktona bila moguća, potrebno je razjasniti put biosinteze. Provedena je funkcionalna karakterizacija gena kandidata iz A. montana, koji su potencijalno uključeni u biosintetski put seskviterpenskih laktona, u heterolognom ekspresijskom sustavu, kvascu Saccharomyces cerevisiae. Metaboliti sintetizirani u kvascu analizirani su plinskom kromatografijom i masenom spektrometrijom. Također, analizirani su ekspresijski obrasci gena kandidata te distribucija i sadržaj seskviterpenskih laktona u različitim tkivima biljke A. montana. Potvrđeno je da geni kandidati AmGAS i AmGAO kodiraju sintazu germakrina A, odnosno oksidazu germakrina A te je potvrđena njihova enzimska aktivnost. Geni AmCYP su djelomično funkcionalno okarakterizirani, a gen AmCYP7 pokazao je najveći potencijal za daljnje istraživanje. Korelacija ekspresijskih obrazaca gena kandidata i sadržaja bioaktivnih sastojaka u tkivima biljke A. montana pokazala je da je gen AmCYP7 vjerojatno uključen u biosintezu seskviterpenskih laktona i pružila preliminarne dokaze za biosintezu seskviterpenskih laktona u različitim razvojnim stadijima biljke A. montana.
Keywords
Keywords (croatian)
Languageenglish
URN:NBNurn:nbn:hr:217:279243
Study programmeTitle: Molecular Biology
Study programme type: university
Study level: graduate
Academic / professional title: magistar/magistra molekularne biologije (magistar/magistra molekularne biologije)
Type of resourceText
File originBorn digital
Access conditionsOpen access
Embargo expiration date: 2024-02-28
Terms of useLicence In copyright icon
Created on2022-03-16 10:21:08