prikaz prve stranice dokumenta Učinkovitost somatske embriogeneze u mutantima uročnjaka (Arabidopsis thaliana L.) s narušenim mehanizmom metilacije DNA
  Download
PDF 2 MB
master's thesis
Učinkovitost somatske embriogeneze u mutantima uročnjaka (Arabidopsis thaliana L.) s narušenim mehanizmom metilacije DNA
Zagreb: University of Zagreb, Faculty of Science, 2022. urn:nbn:hr:217:550503
Ivanić, Ariana
University of Zagreb
Faculty of Science
Department of Biology

Institutional repository: Repository of the Faculty of Science

Cite this document

Ivanić, A. (2022). Učinkovitost somatske embriogeneze u mutantima uročnjaka (Arabidopsis thaliana L.) s narušenim mehanizmom metilacije DNA (Master's thesis). Zagreb: University of Zagreb, Faculty of Science. Retrieved from https://urn.nsk.hr/urn:nbn:hr:217:550503

Ivanić, Ariana. "Učinkovitost somatske embriogeneze u mutantima uročnjaka (Arabidopsis thaliana L.) s narušenim mehanizmom metilacije DNA." Master's thesis, University of Zagreb, Faculty of Science, 2022. https://urn.nsk.hr/urn:nbn:hr:217:550503

Ivanić, Ariana. "Učinkovitost somatske embriogeneze u mutantima uročnjaka (Arabidopsis thaliana L.) s narušenim mehanizmom metilacije DNA." Master's thesis, University of Zagreb, Faculty of Science, 2022. https://urn.nsk.hr/urn:nbn:hr:217:550503

Ivanić, A. (2022). 'Učinkovitost somatske embriogeneze u mutantima uročnjaka (Arabidopsis thaliana L.) s narušenim mehanizmom metilacije DNA', Master's thesis, University of Zagreb, Faculty of Science, accessed 24 May 2024, https://urn.nsk.hr/urn:nbn:hr:217:550503

Ivanić A. Učinkovitost somatske embriogeneze u mutantima uročnjaka (Arabidopsis thaliana L.) s narušenim mehanizmom metilacije DNA [Master's thesis]. Zagreb: University of Zagreb, Faculty of Science; 2022 [cited 2024 May 24] Available at: https://urn.nsk.hr/urn:nbn:hr:217:550503

A. Ivanić, "Učinkovitost somatske embriogeneze u mutantima uročnjaka (Arabidopsis thaliana L.) s narušenim mehanizmom metilacije DNA", Master's thesis, University of Zagreb, Faculty of Science, Zagreb, 2022. Available at: https://urn.nsk.hr/urn:nbn:hr:217:550503

Metadata
TitleUčinkovitost somatske embriogeneze u mutantima uročnjaka (Arabidopsis thaliana L.) s narušenim mehanizmom metilacije DNA
Title (english)Somatic embryogenesis efficiency in DNA methylation mutants of Arabidopsis thaliana L.
AuthorAriana Ivanić
MentorDunja Leljak-Levanić (mentor)
Committee memberDunja Leljak-Levanić (predsjednik povjerenstva)
Committee memberŽeljka Vidaković-Cifrek (član povjerenstva)
Committee memberBiljana Balen (član povjerenstva)
Committee memberPetra Peharec Štefanić (član povjerenstva)
GranterUniversity of Zagreb
Faculty of Science
(Department of Biology)
Zagreb
Defense date and country2022-02-24, Croatia
Scientific / art field,
discipline and subdiscipline		NATURAL SCIENCES
Biology
Abstract
Somatska embriogeneza (SE) je proces kojim se iz somatske stanice biljke razvija somatski embrij te se naposljetku regenerira čitava biljka. U regulaciji embriogeneze ključnu ulogu ima mehanizam metilacije DNA koji regulira ekspresiju gena i održava stabilnost genoma. Za razliku od animalnih organizama, biljke sadržavaju robusnije mehanizme metilacije DNA te se u njihovom genomu može metilirati bilo koji citozin. Mehanizam metilacije DNA usmjeren malim molekulama RNA (engl. RNA-directed DNA methylation, RdDM) je mehanizam metilacije DNA de novo koji se specifično razvio u biljkama. Poznato je da mutanti za neku od komponenti mehanizma RdDM ispoljavaju aberantni fenotip u različitim stadijima reproduktivnog razvoja tijekom zigotne embriogeneze (ZE), koja dijeli puno sličnosti sa SE. U ovome radu istražena je povezanost mehanizma RdDM i embriogenog potencijala na modelu SE u uročnjaku. Pokazano je da potencijal za indukciju SE ovisi o funkcionalnosti mehanizma RdDM, a tijekom faze sazrijevanja somatskih embrija značaj RdDM-a opada. Ustanovljeno je kako je ekspresija gena povezanih s embriogenezom LEC2, RIE1, MYB115 i ECA1 pod utjecajem mehanizma RdDM. Analizom relativne ekspresije gena LEC2 i RIE1 pokazano je da je kontrola ekspresije LEC2 ovisna o funkcionalnosti RdDM-a tijekom obje faze SE, dok kontrola ekspresije RIE1 o funkcionalnosti RdDM-a ovisi samo tijekom sazrijevanja somatskih embrija.
Abstract (english)
Somatic embryogenesis (SE) is a process in which a somatic plant cell develops into a somatic embryo from which a whole plant can then be regenerated. Mechanism of DNA methylation has a fundamental role in embryogenesis regulation by means of regulating gene expression and maintaining genome stability. Unlike animals, plants possess more robust mechanisms of DNA methylation which can methylate virtually any cytosine in the genome. RNA-directed DNA methylation pathway (RdDM) is a mechanism of DNA methylation de novo specific for plants. Previous research has shown that RdDM mutants show aberrant phenotype in different stages of reproductive development during zygotic embryogenesis (ZE), a process which has many similarities with SE. Here an analysis of embryogenic potential and its dependence on a functional RdDM pathway using a model of SE in Arabidopsis thaliana was conducted. It was shown that the potential for induction of SE depended on a functional RdDM pathway while somatic embryo maturation was less dependent on it. It was also shown that expression of embryogenesis-related genes LEC2, RIE1, MYB115 and ECA1 is regulated by RdDM pathway. Analysis of relative gene expression of LEC2 and RIE1 was performed. Control of LEC2 gene expression was dependent on a functional RdDM pathway during both phases of SE, while control of RIE1 gene expression depended on RdDM pathway only during the maturation phase.
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:217:550503
Study programmeTitle: Molecular Biology
Study programme type: university
Study level: graduate
Academic / professional title: magistar/magistra molekularne biologije (magistar/magistra molekularne biologije)
Type of resourceText
File originBorn digital
Access conditionsOpen access
Terms of useLicence In copyright icon
Created on2022-03-17 18:34:35