prikaz prve stranice dokumenta Lipidna peroksidacija i redoks status askorbata tijekom stresom-inducirane somatske embriogeneze bundeve
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master's thesis
Lipidna peroksidacija i redoks status askorbata tijekom stresom-inducirane somatske embriogeneze bundeve
Zagreb: University of Zagreb, Faculty of Science, 2012. urn:nbn:hr:217:660714
Paulišić, Sandi
University of Zagreb
Faculty of Science
Department of Biology

Institutional repository: Repository of the Faculty of Science

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Paulišić, S. (2012). Lipidna peroksidacija i redoks status askorbata tijekom stresom-inducirane somatske embriogeneze bundeve (Master's thesis). Zagreb: University of Zagreb, Faculty of Science. Retrieved from https://urn.nsk.hr/urn:nbn:hr:217:660714

Paulišić, Sandi. "Lipidna peroksidacija i redoks status askorbata tijekom stresom-inducirane somatske embriogeneze bundeve." Master's thesis, University of Zagreb, Faculty of Science, 2012. https://urn.nsk.hr/urn:nbn:hr:217:660714

Paulišić, Sandi. "Lipidna peroksidacija i redoks status askorbata tijekom stresom-inducirane somatske embriogeneze bundeve." Master's thesis, University of Zagreb, Faculty of Science, 2012. https://urn.nsk.hr/urn:nbn:hr:217:660714

Paulišić, S. (2012). 'Lipidna peroksidacija i redoks status askorbata tijekom stresom-inducirane somatske embriogeneze bundeve', Master's thesis, University of Zagreb, Faculty of Science, accessed 22 November 2024, https://urn.nsk.hr/urn:nbn:hr:217:660714

Paulišić S. Lipidna peroksidacija i redoks status askorbata tijekom stresom-inducirane somatske embriogeneze bundeve [Master's thesis]. Zagreb: University of Zagreb, Faculty of Science; 2012 [cited 2024 November 22] Available at: https://urn.nsk.hr/urn:nbn:hr:217:660714

S. Paulišić, "Lipidna peroksidacija i redoks status askorbata tijekom stresom-inducirane somatske embriogeneze bundeve", Master's thesis, University of Zagreb, Faculty of Science, Zagreb, 2012. Available at: https://urn.nsk.hr/urn:nbn:hr:217:660714

Metadata
TitleLipidna peroksidacija i redoks status askorbata tijekom stresom-inducirane somatske embriogeneze bundeve
Title (english)Lipid peroxidation and ascorbate redox state in stress-induced somatic embryogenesis of pumpkin
AuthorSandi Paulišić
MentorSnježana Mihaljević (mentor)
MentorDunja Leljak-Levanić (mentor)
Committee memberDunja Leljak-Levanić (predsjednik povjerenstva)
Committee memberSnježana Mihaljević (član povjerenstva)
Committee memberSandra Radić Brkanac (član povjerenstva)
Committee memberNataša Bauer (član povjerenstva)
GranterUniversity of Zagreb
Faculty of Science
(Department of Biology)
Zagreb
Defense date and country2012-12-17, Croatia
Scientific / art field,
discipline and subdiscipline		NATURAL SCIENCES
Biology
Abstract
Somatska embriogeneza specifičan je oblik stresom-induciranog morfološkog odgovora u biljaka, a u bundeve (Cucurbita pepo L.) moguće ju je potaknuti na podlozi s dodatkom 2,4-D, ali i na podlozi bez hormona s dodatkom 1 mmol/L NH4+ kao jedinim izvorom dušika. Dok se o učincima 2,4-D na somatsku embriogenezu u biljaka zna dosta, fiziološki i morfogeni učinci asimilacije NH4+ na regulaciju somatske embriogeneze tek se istražuju. Analizirana je aktivnost askorbat peroksidaze, redoks status askorbata i lipidna peroksidacija u embriogenom tkivu bundeve. Utvrđeno je da je specifična aktivnost APX u obrnutoj korelaciji s veličinom i mitotskim indeksom embriogenih stanica raslih na podlozi s NH4+, dok je sadržaj L-AA bio manji u tkivu raslom na podlozi s NH4+ nego s 2,4-D. Povećan udio DHA izmjeren je nakon presađivanja tkiva s MS podloga koje sadrže NH4+ ili 2,4D na podlogu MS s nepromijenjenim izvorima dušika i bez hormona, koja pospješuje rast embriogenog tkiva i razvitak starijih stadija embrija. Nije izmjerena značajnija razina lipidne peroksidacije u embriogenom tkivu, neovisno o tretmanu.
Abstract (english)
Somatic embryogenesis represents specific form of stress-induced morphogenic response in plants, and in pumpkin (Cucurbita pepo L.) it can be induced on medium with 4,5 μmol/L 2,4-D and on medium without hormones but with 1 mmol/L NH4+ as the sole source of nitrogen. The effects of 2,4-D on somatic embryogenesis are well known, but physiological and morphogenic aspects of NH4+ assimilation on regulation of somatic embryogenesis are still being investigated. Ascorbate peroxidase activity, redox state of ascorbate and lipid peroxidation were analyzed in pumpkin embryogenic tissue. Results confirmed inverse correlation between specific APX activity and cell size and elongation of embryogenic cells grown on medium with NH4+, while L-AA content was lower in tissue grown on NH4+ then on 2,4-D. The increased L-AA content was measured after transplantation of tissue from MS medium containing NH4+ or 2,4-D to MS medium with unaltered nitrogen sources and without hormones. Lipid peroxidation measured in embryogenic tissue was not significant, regardless of tretment.
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:217:660714
Study programmeTitle: Molecular Biology
Study programme type: university
Study level: graduate
Academic / professional title: magistar/magistra molekularne biologije (magistar/magistra molekularne biologije)
Type of resourceText
File originBorn digital
Access conditionsAccess restricted to students and staff of home institution
Terms of useLicence In copyright icon
Created on2017-05-02 10:20:46