prikaz prve stranice dokumenta Imunohistokemijska vizualizacija proteina SOX2, SOX17 i CALCA u tumoru zametnih stanica sjemenika čovjeka
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master's thesis
Imunohistokemijska vizualizacija proteina SOX2, SOX17 i CALCA u tumoru zametnih stanica sjemenika čovjeka
Zagreb: University of Zagreb, Faculty of Science, 2022. urn:nbn:hr:217:533739
Malnar, Marina
University of Zagreb
Faculty of Science
Department of Biology

Institutional repository: Repository of the Faculty of Science

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Malnar, M. (2022). Imunohistokemijska vizualizacija proteina SOX2, SOX17 i CALCA u tumoru zametnih stanica sjemenika čovjeka (Master's thesis). Zagreb: University of Zagreb, Faculty of Science. Retrieved from https://urn.nsk.hr/urn:nbn:hr:217:533739

Malnar, Marina. "Imunohistokemijska vizualizacija proteina SOX2, SOX17 i CALCA u tumoru zametnih stanica sjemenika čovjeka." Master's thesis, University of Zagreb, Faculty of Science, 2022. https://urn.nsk.hr/urn:nbn:hr:217:533739

Malnar, Marina. "Imunohistokemijska vizualizacija proteina SOX2, SOX17 i CALCA u tumoru zametnih stanica sjemenika čovjeka." Master's thesis, University of Zagreb, Faculty of Science, 2022. https://urn.nsk.hr/urn:nbn:hr:217:533739

Malnar, M. (2022). 'Imunohistokemijska vizualizacija proteina SOX2, SOX17 i CALCA u tumoru zametnih stanica sjemenika čovjeka', Master's thesis, University of Zagreb, Faculty of Science, accessed 29 November 2024, https://urn.nsk.hr/urn:nbn:hr:217:533739

Malnar M. Imunohistokemijska vizualizacija proteina SOX2, SOX17 i CALCA u tumoru zametnih stanica sjemenika čovjeka [Master's thesis]. Zagreb: University of Zagreb, Faculty of Science; 2022 [cited 2024 November 29] Available at: https://urn.nsk.hr/urn:nbn:hr:217:533739

M. Malnar, "Imunohistokemijska vizualizacija proteina SOX2, SOX17 i CALCA u tumoru zametnih stanica sjemenika čovjeka", Master's thesis, University of Zagreb, Faculty of Science, Zagreb, 2022. Available at: https://urn.nsk.hr/urn:nbn:hr:217:533739

Metadata
TitleImunohistokemijska vizualizacija proteina SOX2, SOX17 i CALCA u tumoru zametnih stanica sjemenika čovjeka
Title (english)Immunohistochemical visualization of SOX2, SOX17 and CALCA proteins in human testicular germ cell tumor
AuthorMarina Malnar
MentorNino Sinčić (mentor)
MentorRomana Gračan (sumentor)
Committee memberJasna Lajtner (predsjednik povjerenstva)
Committee memberInes Radanović (član povjerenstva)
Committee memberDraginja Mrvoš-Sermek (član povjerenstva)
Committee memberNenad Judaš (član povjerenstva)
GranterUniversity of Zagreb
Faculty of Science
(Department of Biology)
Zagreb
Defense date and country2022-04-12, Croatia
Scientific / art field,
discipline and subdiscipline		NATURAL SCIENCES
Biology
Abstract
Tumori sjemenika su najčešći tumori koji pogađaju muškarce u najreproduktivnijoj dobi od 15 do 45 godina. Tumori zametnih stanica sjemenika (engl. testicular germ cell tumor, TGCT) dijagnosticiraju se u oko 95 % slučajeva, što ih čini najčešćim oblikom tumora sjemenika. Kao vrlo heterogena skupina neoplazija, ugrubo se dijele na seminome i neseminome, a nerijetko se javljaju u obliku miješanih tumora zametnih stanica. Geni SOX2 i SOX17 su uključeni u regulaciju diferencijacije embrionalnih stanica i danas se koriste kao tkivni biomarkeri u dijagnostici embrionalnog karcinoma i seminoma. CALCA je gen supresor tumora, čija je ekspresija poremećena u nekoliko vrsta tumora. U ovom je istraživanju metodom imunohistokemije istražena količina i lokalizacija proteina SOX2, SOX17 i CALCA u tkivu sjemenika ljudi s TGCT-om (N=38), kao i u tkivu sjemenika bez neoplastičnih promjena (N=20). Protein CALCA je pokazao veliki dijagnostički potencijal za otkrivanje koriokarcinoma, koji je najagresivniji tip TGCT-a. Koekspresija SOX17 i CALCA, a odsutstvo proteina SOX2, se pokazala potencijalnim biomarkerom u dijagnosticiranju neoplazije zametnih stanica in situ koja je preteča zloćudnih TGCT-a.
Abstract (english)
Testicular tumors are the most common type of tumors affecting men in their most reproductive ages, from 15 to 45 years. Testicular germ cell tumors (TGCTs) are diagnosed in about 95 % of cases, making them the most common form of testicular tumors. As a very heterogeneus group of neoplasms, they are roughly divided into seminomas and nonseminomas, and often occur in the form of mixed germ cell tumors. The SOX2 and SOX17 genes are involved in the regulation of embryonic cell differentiation and they are used as tissue biomarkers in current diagnosis of embryonal carcinoma and seminoma. CALCA gene is a tumor suppressor gene whose expression is disrupted in several types of tumors. In this study, the amount and localization of SOX2, SOX17 and CALCA proteins were investigated by immunohistochemical method in testicular tissue of people with TGCT (N=38) and in testicular tissue without neoplastic changes (N=20). The CALCA protein has shown great diagnostic potential for detecting choriocarcinoma, which is the most aggressive type of TGCT. Coexpression of SOX17 and CALCA proteins, with the absence of SOX2 protein, may be used as a potential biomarkers in the diagnosis of germ cell neoplasia in situ considered as a precursor of malignant TGCTs.
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:217:533739
Study programmeTitle: Integrated undergraduate and graduate university study of Biology and Chemistry Education; specializations in: Integrated undergraduate and graduate university study of Biology and Chemistry Education
Course: Integrated undergraduate and graduate university study of Biology and Chemistry Education
Study programme type: university
Study level: integrated undergraduate and graduate
Academic / professional title: magistar/magistra edukacije biologije i kemije (magistar/magistra edukacije biologije i kemije)
Type of resourceText
File originBorn digital
Access conditionsOpen access
Embargo expiration date: 2023-04-12
Terms of useLicence In copyright icon
Created on2022-04-28 09:13:00