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master's thesis
Usporedba različitih postupaka izdvajanja virusne RNA iz tkiva ptičjeg mozga
Zagreb: University of Zagreb, Faculty of Science, 2012. urn:nbn:hr:217:950519
Podgorski, Iva
University of Zagreb
Faculty of Science
Department of Biology

Institutional repository: Repository of the Faculty of Science

Cite this document

Podgorski, I. (2012). Usporedba različitih postupaka izdvajanja virusne RNA iz tkiva ptičjeg mozga (Master's thesis). Zagreb: University of Zagreb, Faculty of Science. Retrieved from https://urn.nsk.hr/urn:nbn:hr:217:950519

Podgorski, Iva. "Usporedba različitih postupaka izdvajanja virusne RNA iz tkiva ptičjeg mozga." Master's thesis, University of Zagreb, Faculty of Science, 2012. https://urn.nsk.hr/urn:nbn:hr:217:950519

Podgorski, Iva. "Usporedba različitih postupaka izdvajanja virusne RNA iz tkiva ptičjeg mozga." Master's thesis, University of Zagreb, Faculty of Science, 2012. https://urn.nsk.hr/urn:nbn:hr:217:950519

Podgorski, I. (2012). 'Usporedba različitih postupaka izdvajanja virusne RNA iz tkiva ptičjeg mozga', Master's thesis, University of Zagreb, Faculty of Science, accessed 22 November 2024, https://urn.nsk.hr/urn:nbn:hr:217:950519

Podgorski I. Usporedba različitih postupaka izdvajanja virusne RNA iz tkiva ptičjeg mozga [Master's thesis]. Zagreb: University of Zagreb, Faculty of Science; 2012 [cited 2024 November 22] Available at: https://urn.nsk.hr/urn:nbn:hr:217:950519

I. Podgorski, "Usporedba različitih postupaka izdvajanja virusne RNA iz tkiva ptičjeg mozga", Master's thesis, University of Zagreb, Faculty of Science, Zagreb, 2012. Available at: https://urn.nsk.hr/urn:nbn:hr:217:950519

Metadata
TitleUsporedba različitih postupaka izdvajanja virusne RNA iz tkiva ptičjeg mozga
Title (english)Comparison of different methods for extraction of viral RNA from avian brain tissue
AuthorIva Podgorski
MentorVladimir Savić (mentor)
MentorInga Marijanović (mentor)
Committee memberInga Marijanović (predsjednik povjerenstva)
Committee memberVladimir Savić (član povjerenstva)
Committee memberMartina Šeruga Musić (član povjerenstva)
Committee memberDijana Škorić (član povjerenstva)
GranterUniversity of Zagreb
Faculty of Science
(Department of Biology)
Zagreb
Defense date and country2012-07-03, Croatia
Scientific / art field,
discipline and subdiscipline		NATURAL SCIENCES
Biology
Abstract
Brza i točna dijagnostika zaraznih bolesti životinja vrlo je bitna radi pravodobnog sprječavanja širenja bolesti i posljedičnih velikih gubitaka. Tehnike molekularne biologije, posebno izdvajanje RNA i DNA, nalaze veliku primjenu u dijagnostici virusnih infekcija. U ovom radu je istražen učinak uranjanja uzoraka u tekući dušik i uspješnost 4 različite metode izdvajanja virusne RNA iz tkiva mozga pokusno zaraženih pilića koje je pohranjeno na -70°C. RNA je izdvojena korištenjem komercijalnih reagensa: 1) Roche High Pure Viral Nucleic Acid Kit, 2) TRIzol®, 3) Qiagen RNeasy Lipid Tissue Mini Kit te 4) kombinacijom TRIzol® i Roche High Pure Viral Nucleic Acid Kita. Prinosi izdvojene RNA su uspoređeni RRT-PCR analizom na uređaju LightCycler 1.5. Svaka metoda je dala dovoljnu količinu RNA za potvrdu prisutnosti ptičjeg paramiksovirusa tipa 1, ali najbolji prinosi dobiveni su korištenjem komercijalnog kompleta Qiagen RNeasy Lipid Tissue Mini Kit i korištenjem reagensa TRIzol®. Uranjanje tkiva u tekući dušik nije imalo utjecaja na prinos RNA.
Abstract (english)
Rapid and accurate diagnosis of animal infectious diseases is cruical for timely prevention of the spread and the consequent heavy losses. Molecular biology techniques, especially RNA and DNA isolation, are extensively used in viral infection diagnosis. This study investigates the effect of sample freezing in liquid nitrogen and viral RNA yield by use of four different RNA isolation methods from experimentally infected chickensʼ brain tissue previously stored at -70°C. RNA was isolated by using commercial reagents: 1) Roche High Pure Viral Nucleic Acid Kit, 2) TRIzol®, 3) Qiagen RNeasy Lipid Tissue Mini Kit and 4) combination of TRIzol® and Roche High Pure Viral Nucleic Acid Kit. Yields of isolated RNA were compared by RRT-PCR in LightCycler 1.5. Each method provided enough RNA to detect avian paramyxovirus type 1, but isolation by using Qiagen RNeasy Lipid Tissue Mini Kit and TRIzol® gave the best RNA yield. Freezing of the brain tissue in liquid nitrogen did not influence the yield of RNA.
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:217:950519
Study programmeTitle: Molecular Biology
Study programme type: university
Study level: graduate
Academic / professional title: magistar/magistra molekularne biologije (magistar/magistra molekularne biologije)
Type of resourceText
File originBorn digital
Access conditionsAccess restricted to students and staff of home institution
Terms of useLicence In copyright icon
Created on2017-05-02 10:29:59