prikaz prve stranice dokumenta Učinak flavonoida kvercetina na staničnu smrt izazvanu vodikovim peroksidom u kulturi neurona P19
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master's thesis
Učinak flavonoida kvercetina na staničnu smrt izazvanu vodikovim peroksidom u kulturi neurona P19
Zagreb: University of Zagreb, Faculty of Science, 2012. urn:nbn:hr:217:903595
Puhović, Jasmina
University of Zagreb
Faculty of Science
Department of Biology

Institutional repository: Repository of the Faculty of Science

Cite this document

Puhović, J. (2012). Učinak flavonoida kvercetina na staničnu smrt izazvanu vodikovim peroksidom u kulturi neurona P19 (Master's thesis). Zagreb: University of Zagreb, Faculty of Science. Retrieved from https://urn.nsk.hr/urn:nbn:hr:217:903595

Puhović, Jasmina. "Učinak flavonoida kvercetina na staničnu smrt izazvanu vodikovim peroksidom u kulturi neurona P19." Master's thesis, University of Zagreb, Faculty of Science, 2012. https://urn.nsk.hr/urn:nbn:hr:217:903595

Puhović, Jasmina. "Učinak flavonoida kvercetina na staničnu smrt izazvanu vodikovim peroksidom u kulturi neurona P19." Master's thesis, University of Zagreb, Faculty of Science, 2012. https://urn.nsk.hr/urn:nbn:hr:217:903595

Puhović, J. (2012). 'Učinak flavonoida kvercetina na staničnu smrt izazvanu vodikovim peroksidom u kulturi neurona P19', Master's thesis, University of Zagreb, Faculty of Science, accessed 25 November 2024, https://urn.nsk.hr/urn:nbn:hr:217:903595

Puhović J. Učinak flavonoida kvercetina na staničnu smrt izazvanu vodikovim peroksidom u kulturi neurona P19 [Master's thesis]. Zagreb: University of Zagreb, Faculty of Science; 2012 [cited 2024 November 25] Available at: https://urn.nsk.hr/urn:nbn:hr:217:903595

J. Puhović, "Učinak flavonoida kvercetina na staničnu smrt izazvanu vodikovim peroksidom u kulturi neurona P19", Master's thesis, University of Zagreb, Faculty of Science, Zagreb, 2012. Available at: https://urn.nsk.hr/urn:nbn:hr:217:903595

Metadata
TitleUčinak flavonoida kvercetina na staničnu smrt izazvanu vodikovim peroksidom u kulturi neurona P19
Title (english)Effect of flavonoid quercetine on hydrogen peroxide-induced cell death in culture of P19 neurons
AuthorJasmina Puhović
MentorMaja Jazvinšćak Jembrek (mentor)
MentorNada Oršolić (mentor)
Committee memberNada Oršolić (predsjednik povjerenstva)
Committee memberMaja Jazvinšćak Jembrek (član povjerenstva)
Committee memberMaja Matulić (član povjerenstva)
GranterUniversity of Zagreb
Faculty of Science
(Department of Biology)
Zagreb
Defense date and country2012-01-23, Croatia
Scientific / art field,
discipline and subdiscipline		NATURAL SCIENCES
Biology
Abstract
Flavonoid kvercetin ima sposobnost neutralizacije slobodnih radikala te može zaštititi stanice od oksidativnih oštećenja. Stoga se proučava njegov potencijal u prevenciji i terapiji neuroloških poremećaja uzrokovanih oksidativnim stresom. Cilj istraživanja bio je utvrditi učinak kvercetina na staničnu smrt izazvanu vodikovim peroksidom (H2O2) u kulturi neurona P19, dobivenoj diferencijacijom stanica embrionalnog karcinoma miša. Kod stanica izloženih oksidativnom oštećenju kvercetin je povećao udio živih stanica. Utvrdili smo da je u prisutnosti kvercetina smanjeno otpuštanje laktat-dehidrogenaze iz stanica izloženih 1,5 mM H2O2, što upućuje na protekciju od stanične smrti s obilježjima nekroze. Također, u prisutnosti kvercetina je inhibirana aktivacija kaspaza-3/7 izazvana 150 μM H2O2, što ukazuje na antiapoptotsko djelovanje kvercetina. U prisutnosti kvercetina nije bilo promjene u razini mRNA za proteine Bcl-2 i Bax u odnosu na 150 μM H2O2, koji je smanjio ekspresiju mRNA za Bcl-2. Dobiveni rezultati upućuju na neuroprotektivno djelovanje kvercetina u uvjetima oksidacijskog stresa u neuronima P19.
Abstract (english)
Flavonoid quercetin is capable of scavenging free radicals and protecting cells from oxidative injuries. It may provide therapeutic potential for the prevention and treatment of neuronal disorders caused by oxidative stress. In this study we used P19 neurons, originated from the mouse embryonal carcinoma P19 cell line, to investigate the effects of quercetin on H2O2-induced neuronal death. During the oxidative injury, quercetin increased the number of viable cells. Quercetin decreased leakage of lactate dehydrogenase from cells exposed to 1,5 mM H202, suggesting protective role of quercetin on necrosis. Activation of caspase-3/7 induced by 150 μM H202 was inhibited in the presence of quercetin, thereby demostrating its antapoptotic activity. However, it neither affected 150 μM H202- induced decrease in expression of Bcl-2 mRNA, nor the expression of Bax mRNA that was not affected by 150 μM H202. In conclusion, our results indicate neuroprotective potential of quercetin against oxidative stress-induced neuronal cell death.
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:217:903595
Study programmeTitle: Experimental Biology
Study programme type: university
Study level: graduate
Academic / professional title: magistar/magistra eksperimentalne biologije (magistar/magistra eksperimentalne biologije)
Type of resourceText
File originBorn digital
Access conditionsAccess restricted to students and staff of home institution
Terms of useLicence In copyright icon
Created on2017-05-02 10:43:41