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master's thesis
Ekspresija gena MAO i COMT u mozgu gušterica vrsta Podarcis sicula i Podarcis melisellensis
Zagreb: University of Zagreb, Faculty of Science, 2022. urn:nbn:hr:217:435130
Zvonar, Sara
University of Zagreb
Faculty of Science
Department of Biology

Institutional repository: Repository of the Faculty of Science

Cite this document

Zvonar, S. (2022). Ekspresija gena MAO i COMT u mozgu gušterica vrsta Podarcis sicula i Podarcis melisellensis (Master's thesis). Zagreb: University of Zagreb, Faculty of Science. Retrieved from https://urn.nsk.hr/urn:nbn:hr:217:435130

Zvonar, Sara. "Ekspresija gena MAO i COMT u mozgu gušterica vrsta Podarcis sicula i Podarcis melisellensis." Master's thesis, University of Zagreb, Faculty of Science, 2022. https://urn.nsk.hr/urn:nbn:hr:217:435130

Zvonar, Sara. "Ekspresija gena MAO i COMT u mozgu gušterica vrsta Podarcis sicula i Podarcis melisellensis." Master's thesis, University of Zagreb, Faculty of Science, 2022. https://urn.nsk.hr/urn:nbn:hr:217:435130

Zvonar, S. (2022). 'Ekspresija gena MAO i COMT u mozgu gušterica vrsta Podarcis sicula i Podarcis melisellensis', Master's thesis, University of Zagreb, Faculty of Science, accessed 28 November 2024, https://urn.nsk.hr/urn:nbn:hr:217:435130

Zvonar S. Ekspresija gena MAO i COMT u mozgu gušterica vrsta Podarcis sicula i Podarcis melisellensis [Master's thesis]. Zagreb: University of Zagreb, Faculty of Science; 2022 [cited 2024 November 28] Available at: https://urn.nsk.hr/urn:nbn:hr:217:435130

S. Zvonar, "Ekspresija gena MAO i COMT u mozgu gušterica vrsta Podarcis sicula i Podarcis melisellensis", Master's thesis, University of Zagreb, Faculty of Science, Zagreb, 2022. Available at: https://urn.nsk.hr/urn:nbn:hr:217:435130

Metadata
TitleEkspresija gena MAO i COMT u mozgu gušterica vrsta Podarcis sicula i Podarcis melisellensis
Title (english)Expression of MAO and COMT genes in the brain of Podarcis sicula and Podarcis melisellensis lizards
AuthorSara Zvonar
MentorSofia Ana Blažević (mentor)
Committee memberSofia Ana Blažević (predsjednik povjerenstva)
Committee memberDuje Lisičić (član povjerenstva)
Committee memberNataša Bauer (član povjerenstva)
Committee memberDubravka Hranilović (član povjerenstva)
GranterUniversity of Zagreb
Faculty of Science
(Department of Biology)
Zagreb
Defense date and country2022-04-28, Croatia
Scientific / art field,
discipline and subdiscipline		NATURAL SCIENCES
Biology
Abstract
Agresivnost kao oblik kompetitivnog ponašanja česta je pojava koja uvelike utječe na odnos između jedinki, populacija i/ili vrsta. Primijećena je i u međusobnom odnosu jedinki vrsta Podarcis sicula (primorska gušterica) i Podarcis melisellensis (krška gušterica) duž jadranske obale, pri čemu P. sicula kao agresivnija vrsta aktivno potiskuje P. melisellensis, smanjujući njezin areal. Poznato je da se agresivnost javlja kao rezultat djelovanja određenih neurotransmitera u mozgu - dopamina, serotonina i noradrenalina. Cilj ovog rada je analiza ekspresije gena MAO (monoamin oksidaza) i COMT (katehol-O-metiltransferaza) koji razgrađuju dopamin i tako mogu djelovati na njegovu koncentraciju u mozgu jedinki vrsta P. sicula i P. melisellensis. U svrhu istraživanja provedeno je terensko uzorkovanje jedinki na tri lokacije (Pag, Sinj, Knin), njihovo žrtvovanje i pohrana moždanog tkiva iz kojeg je izolirana RNA, dizajn početnica specifičnih za vrstu, reverzna transkripcija (RT-PCR) i kvantitativni PCR (qRT-PCR). Nije pronađena razlika u relativnoj ekspresiji gena MAO i COMT između istraživanih vrsta.
Abstract (english)
Aggression as a form of competitive behavior is a common occurrence that greatly affects the relationship between individuals, populations and/or species. It has also been noted in the relationship between the species Podarcis sicula (coastal lizard) and Podarcis melisellensis (karst lizard) along the Adriatic coast, where more aggressive P. sicula actively suppresses P. melisellensis reducing its living area. It is known that aggression occurs as a result of the action of certain neurotransmitters in the brain - dopamine, serotonin and norepinephrine. The aim of this study was to analyze the expression of MAO (monoamine oxidase) and COMT (catechol-O-methyltransferase) genes that degrade dopamine therefore affecting dopamine levels in the brain of individuals of species P. sicula and P. melisellensis. Field sampling of individuals at three locations (Pag, Sinj, Knin) was performed, their sacrifice and storage of brain tissue from which RNA was isolated, species specific primers designed and reverse transcription (RT-PCR) and quantitative PCR (qRT-PCR) performed. No difference in the relative expression of MAO and COMT genes was found between the studied species.
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:217:435130
Study programmeTitle: Experimental Biology
Study programme type: university
Study level: graduate
Academic / professional title: magistar/magistra eksperimentalne biologije (magistar/magistra eksperimentalne biologije)
Type of resourceText
File originBorn digital
Access conditionsOpen access
Embargo expiration date: 2024-04-28
Terms of useLicence In copyright icon
Created on2022-05-13 08:30:18