prikaz prve stranice dokumenta Metabolomski pristupi u otkrivanju novih biomarkera i dubinska fenotipizacija u bubrežnim i srčanim bolestima u pasa
  Download
PDF 6.99 MB
doctoral thesis
Metabolomski pristupi u otkrivanju novih biomarkera i dubinska fenotipizacija u bubrežnim i srčanim bolestima u pasa
Zagreb: University of Zagreb, Faculty of Science, 2022. urn:nbn:hr:217:971002
Rubić, Ivana
University of Zagreb
Faculty of Science
Department of Chemistry

Institutional repository: Repository of the Faculty of Science

Cite this document

Rubić, I. (2022). Metabolomski pristupi u otkrivanju novih biomarkera i dubinska fenotipizacija u bubrežnim i srčanim bolestima u pasa (Doctoral thesis). Zagreb: University of Zagreb, Faculty of Science. Retrieved from https://urn.nsk.hr/urn:nbn:hr:217:971002

Rubić, Ivana. "Metabolomski pristupi u otkrivanju novih biomarkera i dubinska fenotipizacija u bubrežnim i srčanim bolestima u pasa." Doctoral thesis, University of Zagreb, Faculty of Science, 2022. https://urn.nsk.hr/urn:nbn:hr:217:971002

Rubić, Ivana. "Metabolomski pristupi u otkrivanju novih biomarkera i dubinska fenotipizacija u bubrežnim i srčanim bolestima u pasa." Doctoral thesis, University of Zagreb, Faculty of Science, 2022. https://urn.nsk.hr/urn:nbn:hr:217:971002

Rubić, I. (2022). 'Metabolomski pristupi u otkrivanju novih biomarkera i dubinska fenotipizacija u bubrežnim i srčanim bolestima u pasa', Doctoral thesis, University of Zagreb, Faculty of Science, accessed 21 December 2024, https://urn.nsk.hr/urn:nbn:hr:217:971002

Rubić I. Metabolomski pristupi u otkrivanju novih biomarkera i dubinska fenotipizacija u bubrežnim i srčanim bolestima u pasa [Doctoral thesis]. Zagreb: University of Zagreb, Faculty of Science; 2022 [cited 2024 December 21] Available at: https://urn.nsk.hr/urn:nbn:hr:217:971002

I. Rubić, "Metabolomski pristupi u otkrivanju novih biomarkera i dubinska fenotipizacija u bubrežnim i srčanim bolestima u pasa", Doctoral thesis, University of Zagreb, Faculty of Science, Zagreb, 2022. Available at: https://urn.nsk.hr/urn:nbn:hr:217:971002

Metadata
TitleMetabolomski pristupi u otkrivanju novih biomarkera i dubinska fenotipizacija u bubrežnim i srčanim bolestima u pasa
Title (english)Metabolomic approaches to novel biomarker discovery and deep phenotyping in renal and cardiac diseases in dogs
AuthorIvana Rubić
MentorVladimir Mrljak (mentor)
Committee memberMarko Močibob (predsjednik povjerenstva)
Committee memberVlasta Drevenkar (član povjerenstva)
Committee memberRenata Barić-Rafaj (član povjerenstva)
GranterUniversity of Zagreb
Faculty of Science
(Department of Chemistry)
Zagreb
Defense date and country2022-06-14, Croatia
Scientific / art field,
discipline and subdiscipline		NATURAL SCIENCES
Chemistry
Universal decimal classification
(UDC)		54 - Chemistry. Crystallography. Mineralogy
Abstract
Babezioza i dilatacijska kardiomiopatija važne su bolesti koje se nalaze u pasa, ali i ljudi. Jedna je od najčešćih komplikacija kod babezioze pasa uzrokovane protozoonom Babesia canis akutna ozljeda bubrega. Drugi je po učestalosti uzrok zatajenja srca u pasa idiopatska dilatacijska kardiomiopatija (iDCM) koja rezultira zatajenjem srca ili iznenadnom srčanom smrću zbog nastanaka aritmije. Svrha je istraživanja bila otkriti promjene u metabolomu seruma i urina pasa s promijenjenom funkcijom bubrega kod babezioze, te u metabolomu plazme pasa s iDCM-om u odnosu na zdrave pse. U istraživanju su primijenjeni neciljani i ciljani metabolomski pristup upotrebom tekućinske kromatografije spregnute sa spektrometrijom masa te metabolomska analiza temeljena na plinskoj kromatografiji spregnutoj sa spektrometrijom masa. U metabolomu seruma i urina pasa s babeziozom otkrivene su promjene povezane s oksidacijskim stresom, upalnim odgovorom domaćina, hipoksijom, anemijom i energetskim metabolizmom. U istraživanju iDCM-a identificirane su promjene u metabolomu plazme pasa i poremećaji u metaboličkim putevima koji su uključeni u razvoj iDCM-a.
Abstract (english)
Babesiosis and dilated cardiomyopathy are important diseases in dogs and humans. Acute kidney injury is one of the most common complications in canine babesiosis caused with protozoa Babesia canis. The second most common cause of heart failure in dogs is idiopathic dilated cardiomyopathy (iDCM), which results in heart failure or sudden cardiac death due to arrhythmia. This study aimed to determine changes in the serum and urine metabolome of dogs with renal failure in babesiosis, and in the plasma metabolome of dogs with iDCM compared to healthy dogs. In this study, we applied an untargeted and targeted metabolomics approach using liquid chromatography coupled to mass spectrometry and gas chromatography coupled to mass spectrometry-based metabolomics. In the serum and urine samples of dogs with babesiosis were found differences associated with oxidative stress, inflammatory host response, hypoxia, anemia, and energy metabolism. We discovered changes in canine plasma metabolome and metabolic pathway disorders involved in the development of iDCM.
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:217:971002
Promotion2022
Study programmeTitle: Doctoral study in chemistry
Study programme type: university
Study level: postgraduate
Academic / professional title: doktor/doktorica znanosti, područje prirodnih znanosti, polje kemija (doktor/doktorica znanosti, područje prirodnih znanosti, polje kemija)
Type of resourceText
ExtentLXXXVII, 149 str. ; 30 cm
File originBorn digital
Access conditionsOpen access
Terms of useLicence In copyright icon
Created on2022-06-30 14:36:34