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doctoral thesis
Stabilnost i toksični učinci specija arsena u uzorcima voda
Zagreb: University of Zagreb, Faculty of Science, 2022. urn:nbn:hr:217:885388
Alispahić, Amra
University of Zagreb
Faculty of Science
Department of Chemistry

Institutional repository: Repository of the Faculty of Science

Cite this document

Alispahić, A. (2022). Stabilnost i toksični učinci specija arsena u uzorcima voda (Doctoral thesis). Zagreb: University of Zagreb, Faculty of Science. Retrieved from https://urn.nsk.hr/urn:nbn:hr:217:885388

Alispahić, Amra. "Stabilnost i toksični učinci specija arsena u uzorcima voda." Doctoral thesis, University of Zagreb, Faculty of Science, 2022. https://urn.nsk.hr/urn:nbn:hr:217:885388

Alispahić, Amra. "Stabilnost i toksični učinci specija arsena u uzorcima voda." Doctoral thesis, University of Zagreb, Faculty of Science, 2022. https://urn.nsk.hr/urn:nbn:hr:217:885388

Alispahić, A. (2022). 'Stabilnost i toksični učinci specija arsena u uzorcima voda', Doctoral thesis, University of Zagreb, Faculty of Science, accessed 22 November 2024, https://urn.nsk.hr/urn:nbn:hr:217:885388

Alispahić A. Stabilnost i toksični učinci specija arsena u uzorcima voda [Doctoral thesis]. Zagreb: University of Zagreb, Faculty of Science; 2022 [cited 2024 November 22] Available at: https://urn.nsk.hr/urn:nbn:hr:217:885388

A. Alispahić, "Stabilnost i toksični učinci specija arsena u uzorcima voda", Doctoral thesis, University of Zagreb, Faculty of Science, Zagreb, 2022. Available at: https://urn.nsk.hr/urn:nbn:hr:217:885388

Metadata
TitleStabilnost i toksični učinci specija arsena u uzorcima voda
Title (english)Stability and toxic effects of arsenic species in water samples
AuthorAmra Alispahić
MentorAdela Krivohlavek (mentor)
MentorNives Galić (komentor)
Committee memberIva Juranović Cindrić (predsjednik povjerenstva)
Committee memberIvone Jakaša (član povjerenstva)
Committee memberGordana Pehnec (član povjerenstva)
Committee memberSanda Rončević (član povjerenstva)
GranterUniversity of Zagreb
Faculty of Science
(Department of Chemistry)
Zagreb
Defense date and country2022-07-12, Croatia
Scientific / art field,
discipline and subdiscipline		NATURAL SCIENCES
Chemistry
Universal decimal classification
(UDC)		54 - Chemistry. Crystallography. Mineralogy
Abstract
U ovom radu ispitana je stabilnost omjera anorganskih specija arsena As(III)/As(V) u modelnim otopinama i uzorcima prirodnih podzemnih voda s četiri lokacije u Hrvatskoj uz dodatak sljedećih konzervansa: limunska kiselina, natrijev citrat, kalijev oksalat, kalijev–natrijev tartarat i octena kiselina. Specijacijska analiza provedena je metodom voltametrije anodnog otapanja i UPLC–ICP–MS. Utvrđeno je kako kombinacija ispitanih konzervansa s octenom kiselinom uspješno stabilizira omjer specija arsena te je on u modelnim otopinama stabilan do 8 dana, dok je u uzorcima prirodnih voda stabilan 6 do 7 dana. Akutnim testom ekotoksičnosti određena je vrijednost LC50 nakon 24 h na Daphnia magna. Dobivene vrijednosti su 5,07 ppm za As(III), 10,27 ppm za As(V) i 0,58 ppm za As(III) s nanočesticama željeza. MTT testom citotoksičnosti na HepG2 staničnoj liniji određena je vrijednosti LC50 nakon 24 h. Najveća citotoksičnost određena je za As(III) uz dodatak SPIO nanočestica, dok As(V) ne inducira staničnu smrt.
Abstract (english)
In this paper, the stability of inorganic arsenic species ratio As(III)/As(V) in model solutions and natural groundwater samples from four locations in Croatia was investigated. Selected preservatives were citric acid, sodium citrate, potassium oxalate, potassium sodium tartrate and acetic acid. Speciation analysis was performed by anodic stripping voltammetry method and UPLC–ICP–MS. It was shown that the combination of the tested preservatives with acetic acid stabilize the arsenic species ratio for up to 8 days in model solutions and for 6 to 7 days in natural water samples. The LC50 values determined by acute ecotoxicity test on Daphnia magna after 24 h amounted to 5.07, 10.27 and 0.58 ppm for As(III), As(V), As(III)/iron nanoparticles, respectively. Cytotoxicity was determined by MTT test on human hepatocarcinoma cell line (HepG2) after 24 h and expressed as LC50 value. The most pronounced effect was observed for As(III)/Fe nanoparticles, while As(V) was inactive.
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:217:885388
Promotion2022
Study programmeTitle: Doctoral study in chemistry
Study programme type: university
Study level: postgraduate
Academic / professional title: doktor/doktorica znanosti, područje prirodnih znanosti, polje kemija (doktor/doktorica znanosti, područje prirodnih znanosti, polje kemija)
Type of resourceText
ExtentXLIX, 128 str. ; 30 cm
File originBorn digital
Access conditionsOpen access
Embargo expiration date: 2024-07-12
Terms of useLicence In copyright icon
Created on2022-07-12 16:33:16