prikaz prve stranice dokumenta Razvoj muških reproduktivnih organa Wistar štakora prenatalno izloženih alfa-cipermetrinu
  Download
PDF 2.97 MB
master's thesis
Razvoj muških reproduktivnih organa Wistar štakora prenatalno izloženih alfa-cipermetrinu
Zagreb: University of Zagreb, Faculty of Science, 2022. urn:nbn:hr:217:269343
Merčep, Antonia
University of Zagreb
Faculty of Science
Department of Biology

Institutional repository: Repository of the Faculty of Science

Cite this document

Merčep, A. (2022). Razvoj muških reproduktivnih organa Wistar štakora prenatalno izloženih alfa-cipermetrinu (Master's thesis). Zagreb: University of Zagreb, Faculty of Science. Retrieved from https://urn.nsk.hr/urn:nbn:hr:217:269343

Merčep, Antonia. "Razvoj muških reproduktivnih organa Wistar štakora prenatalno izloženih alfa-cipermetrinu." Master's thesis, University of Zagreb, Faculty of Science, 2022. https://urn.nsk.hr/urn:nbn:hr:217:269343

Merčep, Antonia. "Razvoj muških reproduktivnih organa Wistar štakora prenatalno izloženih alfa-cipermetrinu." Master's thesis, University of Zagreb, Faculty of Science, 2022. https://urn.nsk.hr/urn:nbn:hr:217:269343

Merčep, A. (2022). 'Razvoj muških reproduktivnih organa Wistar štakora prenatalno izloženih alfa-cipermetrinu', Master's thesis, University of Zagreb, Faculty of Science, accessed 13 May 2024, https://urn.nsk.hr/urn:nbn:hr:217:269343

Merčep A. Razvoj muških reproduktivnih organa Wistar štakora prenatalno izloženih alfa-cipermetrinu [Master's thesis]. Zagreb: University of Zagreb, Faculty of Science; 2022 [cited 2024 May 13] Available at: https://urn.nsk.hr/urn:nbn:hr:217:269343

A. Merčep, "Razvoj muških reproduktivnih organa Wistar štakora prenatalno izloženih alfa-cipermetrinu", Master's thesis, University of Zagreb, Faculty of Science, Zagreb, 2022. Available at: https://urn.nsk.hr/urn:nbn:hr:217:269343

Metadata
TitleRazvoj muških reproduktivnih organa Wistar štakora prenatalno izloženih alfa-cipermetrinu
Title (english)Development of male reproductive organs in Wistar rats prenatally exposed to alfa-cypermethrin
AuthorAntonia Merčep
MentorAnja Katić (mentor)
MentorNada Oršolić (komentor)
Committee memberNada Oršolić (predsjednik povjerenstva)
Committee memberJasna Lajtner (član povjerenstva)
Committee memberMarija Ivković (član povjerenstva)
Committee memberAna Galov (član povjerenstva)
GranterUniversity of Zagreb
Faculty of Science
(Department of Biology)
Zagreb
Defense date and country2022-07-13, Croatia
Scientific / art field,
discipline and subdiscipline		NATURAL SCIENCES
Biology
Abstract
Alfa-cipermetrin je sintetički piretroid tipa II, široke uporabe u poljoprivredi i kućanstvima. Istraživanjima je dokazano da mnogi piretroidi mogu djelovati kao endokrini disruptori, ali za αcipermetrin to još uvijek nije dovoljno istraženo. Testisi su zbog reproduktivne važnosti i osjetljivog razvoja dobar pokazatelj djelovanja endokrinih disruptora. Cilj ovog istraživanja bio je po prvi puta istražiti utječe li peroralna prenatalna izloženost α-cipermetrinu na razvoj muških reproduktivnih organa potomaka Wistar štakora. U tu svrhu provedene su histopatološke, stereološke i imunohistokemijske analize proliferacijskog biljega histona H3 i apoptotičkog biljega pocijepane kaspaze 3 na fetalnim testisima te analize brojnosti i vijabilnosti spermija i procjena očuvanosti spermatogeneze u pubertalnim testisima štakora. S porastom doze α-cipermetrina dolazi do deorganizacije intersticijskog prostora, gubitka cjelovitosti Leydigovih stanica i značajnog smanjenja proliferacije Sertolijevih stanica. Prenatalna izloženost α-cipermetrinu ne utječe na volumensku gustoću gonocita i Sertolijevih stanica i ne uzrokuje njihovu apoptozu u fetalnim testisima, a kod mužjaka u pubertetu ne utječe na brojnost i vijabilnost spermija kao ni na očuvanost spermatogeneze.
Abstract (english)
Alpha-cypermethrin is a synthetic pyrethroid type II widely used in agriculture and households. Studies have shown that synthetic pyrethroids may act as endocrine disruptors, but for αcypermethrin it has not been investigated enough yet. Testes are a good indicator of endocrine disruption due to their reproductive importance and sensitive development. The aim of this study was to investigate for the first time whether oral prenatal exposure to α-cypermethrin affects the development of male reproductive organs of Wistar rat offspring. For this purpose, histopathological, stereological and immunohistochemical analyses of histone H3 proliferation marker and apoptotic marker of cleaved caspase 3 in foetal testes and sperm number and viability analyses as well as assessment of spermatogenesis maintenance in rat pubertal testes were performed. Increase of α-cypermethrin dose leads to disorganisation of the interstitial space, loss of Leydig cells integrity and significant reduction of the Sertoli cells proliferation. Prenatal exposure to α-cypermethrin does not affect the volume density of gonocytes and Sertoli cells and does not cause their apoptosis in foetal testes. Also, it does not affect the number and viability of sperm as well as the maintenance of spermatogenesis in pubertal male rats.
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:217:269343
Study programmeTitle: Experimental Biology
Study programme type: university
Study level: graduate
Academic / professional title: magistar/magistra eksperimentalne biologije (magistar/magistra eksperimentalne biologije)
Type of resourceText
File originBorn digital
Access conditionsOpen access
Terms of useLicence In copyright icon
Created on2022-07-19 09:32:33