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master's thesis
Učinak bakra na genotoksičnost kadmija u duhanu, Nicotiana tabacum L.
Zagreb: University of Zagreb, Faculty of Science, 2011. urn:nbn:hr:217:329095
Ćetković, Maja
University of Zagreb
Faculty of Science
Department of Biology

Institutional repository: Repository of the Faculty of Science

Cite this document

Ćetković, M. (2011). Učinak bakra na genotoksičnost kadmija u duhanu, Nicotiana tabacum L. (Master's thesis). Zagreb: University of Zagreb, Faculty of Science. Retrieved from https://urn.nsk.hr/urn:nbn:hr:217:329095

Ćetković, Maja. "Učinak bakra na genotoksičnost kadmija u duhanu, Nicotiana tabacum L.." Master's thesis, University of Zagreb, Faculty of Science, 2011. https://urn.nsk.hr/urn:nbn:hr:217:329095

Ćetković, Maja. "Učinak bakra na genotoksičnost kadmija u duhanu, Nicotiana tabacum L.." Master's thesis, University of Zagreb, Faculty of Science, 2011. https://urn.nsk.hr/urn:nbn:hr:217:329095

Ćetković, M. (2011). 'Učinak bakra na genotoksičnost kadmija u duhanu, Nicotiana tabacum L.', Master's thesis, University of Zagreb, Faculty of Science, accessed 22 November 2024, https://urn.nsk.hr/urn:nbn:hr:217:329095

Ćetković M. Učinak bakra na genotoksičnost kadmija u duhanu, Nicotiana tabacum L. [Master's thesis]. Zagreb: University of Zagreb, Faculty of Science; 2011 [cited 2024 November 22] Available at: https://urn.nsk.hr/urn:nbn:hr:217:329095

M. Ćetković, "Učinak bakra na genotoksičnost kadmija u duhanu, Nicotiana tabacum L.", Master's thesis, University of Zagreb, Faculty of Science, Zagreb, 2011. Available at: https://urn.nsk.hr/urn:nbn:hr:217:329095

Metadata
TitleUčinak bakra na genotoksičnost kadmija u duhanu, Nicotiana tabacum L.
Title (english)Effect of copper on the genotoxicity of cadmium in tobacco, Nicotiana tabacum L.
AuthorMaja Ćetković
MentorMirjana Pavlica (mentor)
Committee memberMirjana Pavlica (predsjednik povjerenstva)
Committee memberZora Popović (član povjerenstva)
Committee memberZdravko Dolenec (član povjerenstva)
Committee memberVesna Petrović Peroković (član povjerenstva)
GranterUniversity of Zagreb
Faculty of Science
(Department of Biology)
Zagreb
Defense date and country2011-06-27, Croatia
Scientific / art field,
discipline and subdiscipline		NATURAL SCIENCES
Biology
Abstract
Nagli razvoj industrije uzrokuje sve veće zagađenje okoliša. Među brojne okolišne zagađivače ubrajaju se i teški metali. Toksičnost teških metala ovisi o njihovoj koncentraciji, pokretljivosti u biljci i okolišnim uvjetima. Esencijalni metali kao što je bakar, dostupni u prevelikoj koncentraciji postaju toksični za biljku. Kadmij je jedan od neesencijalnih toksičnih teških metala koji je genotoksičan i mutagen za većinu organizama. Cilj istraživanja bio je utvrditi osjetljivost genoma duhana na teške metale bakar i kadmij, pojedinačno i u kombinaciji, metodom komet-testa te istražiti ima li bakar sinergističko ili antagonističko djelovanje na genotoksičnost kadmija. Bioakumulacija istraživanih metala istražena je analizom sadržaja metala u biljnom tkivu tehnikom optičke emisijske spektroskopije uz induktivno spregnutu plazmu. Analiza sadržaja metala pokazala je da je akumulacija kadmija bila viša u korijenu nego u listu biljke duhana. Komet-testom potvrđena je osjetljivost genoma duhana na kadmij i bakar. Celularnim komet-testom uočeno je antagonističko djelovanje bakra na genotoksičnost kadmija u listu, a sinergističko u korijenu. Acelularni komet-test pokazao je da bakar smanjuje genotoksični učinak kadmija u listu.
Abstract (english)
Rapid industry development causes increase of environmental pollution. Among numerous environmental contaminants, heavy metals are of significant value. Toxicity of heavy metals depends on their concentration, mobility in the plant and environmental conditions. Essential metals such as copper, when present in excessive concentration become toxic to plants. Cadmium is one of the nonessential toxic heavy metals that is genotoxic and mutagenic to organisms. The main goal of this study was to determine the sensitivity of the tobacco genome on the heavy metals copper and cadmium, individually and in mixture by comet assay and to investigate whether copper has antagonistic or synergistic effect on the cadmium genotoxicity. Bioaccumulation of heavy metals was measured with inductively coupled plasma atomic emission spectroscopy. Analysis of metal content showed that cadmium accumulation was higher in roots than in leaves of tobacco plants. Comet assay confirmed the sensitivity of the tobacco genome to cadmium and copper. Cellular comet assay showed antagonistic effect of copper on cadmium genotoxicity in leaves, and synergistic effect in roots. Acellular comet test showed that copper reduces genotoxic effects of cadmium in leaves.
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:217:329095
Study programmeTitle: Integrated undergraduate and graduate university study of Biology and Chemistry Education; specializations in: Integrated undergraduate and graduate university study of Biology and Chemistry Education
Course: Integrated undergraduate and graduate university study of Biology and Chemistry Education
Study programme type: university
Study level: integrated undergraduate and graduate
Academic / professional title: magistar/magistra edukacije biologije i kemije (magistar/magistra edukacije biologije i kemije)
Type of resourceText
File originBorn digital
Access conditionsAccess restricted to students and staff of home institution
Terms of useLicence In copyright icon
Created on2017-05-03 07:49:23