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master's thesis
Učinak topline na stabilnost genoma duhana Nicotiana tabacum L.
Zagreb: University of Zagreb, Faculty of Science, 2011. urn:nbn:hr:217:634788
Debogović, Lea
University of Zagreb
Faculty of Science
Department of Biology

Institutional repository: Repository of the Faculty of Science

Cite this document

Debogović, L. (2011). Učinak topline na stabilnost genoma duhana Nicotiana tabacum L. (Master's thesis). Zagreb: University of Zagreb, Faculty of Science. Retrieved from https://urn.nsk.hr/urn:nbn:hr:217:634788

Debogović, Lea. "Učinak topline na stabilnost genoma duhana Nicotiana tabacum L.." Master's thesis, University of Zagreb, Faculty of Science, 2011. https://urn.nsk.hr/urn:nbn:hr:217:634788

Debogović, Lea. "Učinak topline na stabilnost genoma duhana Nicotiana tabacum L.." Master's thesis, University of Zagreb, Faculty of Science, 2011. https://urn.nsk.hr/urn:nbn:hr:217:634788

Debogović, L. (2011). 'Učinak topline na stabilnost genoma duhana Nicotiana tabacum L.', Master's thesis, University of Zagreb, Faculty of Science, accessed 18 May 2024, https://urn.nsk.hr/urn:nbn:hr:217:634788

Debogović L. Učinak topline na stabilnost genoma duhana Nicotiana tabacum L. [Master's thesis]. Zagreb: University of Zagreb, Faculty of Science; 2011 [cited 2024 May 18] Available at: https://urn.nsk.hr/urn:nbn:hr:217:634788

L. Debogović, "Učinak topline na stabilnost genoma duhana Nicotiana tabacum L.", Master's thesis, University of Zagreb, Faculty of Science, Zagreb, 2011. Available at: https://urn.nsk.hr/urn:nbn:hr:217:634788

Metadata
TitleUčinak topline na stabilnost genoma duhana Nicotiana tabacum L.
Title (english)The effect of heat stress on the genetic stability of tobacco Nicotiana tabacum L.
AuthorLea Debogović
MentorMirjana Pavlica (mentor)
Committee memberMirjana Pavlica (predsjednik povjerenstva)
Committee memberTatjana Bakran-Petricioli (član povjerenstva)
Committee memberŽeljka Vidaković-Cifrek (član povjerenstva)
GranterUniversity of Zagreb
Faculty of Science
(Department of Biology)
Zagreb
Defense date and country2011-02-17, Croatia
Scientific / art field,
discipline and subdiscipline		NATURAL SCIENCES
Biology
Abstract
Klimatske promjene jedan su od najvećih izazova s kojima se danas suočavamo. Biljke, kao sesilni organizmi, nemaju mogućnost regulacije temperature ponašanjem, stoga bi stresni uvjeti okoliša kao posljedica globalnog zatopljenja mogli biti katastrofalni za biljke. Cilj istraživanja bio je utvrditi učinak toplinskog stresa na stabilnost genoma duhana. Biljke različite starosti, jedan i tri mjeseca, su bile kratkotrajno (2 h i 4 h) izložene temperaturi od 42 °C i ostavljene na oporavku 3 dana. Razina oštećenja i promjene u molekuli DNA duhana neposredno nakon tretmana i kroz oporavak procijenjeni su primjenom molekularnih metoda komet-test i RAPD. Rezultati komet-testa su pokazali statistički značajna oštećenja DNA neposredno nakon 2 h i 4 h izlaganja povišenoj temperaturi u odnosu na kontrolni uzorak, te popravak oštećenja nakon 3 dana oporavka, bez obzira na starost biljke. Rezultati metode RAPD nisu pokazali razlike u DNA obrascima.
Abstract (english)
Climate change is one of the biggest challenges we face today. Plants are sessile organisms, and as such do not have the ability to regulate their temperature by behavior. Therefore, stressful environmental conditions as a result of global warming may have catastrophic consequences for plants. The objective of this research was to determine the effect of heat stress on the genetic stability of tobacco plants. Plants of different ages, one and three months, were exposed short-term (2 h and 4 h) to temperature of 42 °C and left to recover for 3 days. The extent of DNA damage and DNA alterations after the treatment and during the recovery period were evaluated using the Comet assay and RAPD. Results of the Comet assay showed statistically significant damage to DNA immediately after 2 h and 4 h of exposure to high temperature compared to the control sample, but they also showed that damage was repaired after the 3 days of recovery, regardless of the plant age. RAPD analysis showed no difference in DNA profiles.
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:217:634788
Study programmeTitle: Ecology
Study programme type: university
Study level: graduate
Academic / professional title: magistar/magistra ekologije i zaštite prirode (magistar/magistra ekologije i zaštite prirode)
Type of resourceText
File originBorn digital
Access conditionsAccess restricted to students and staff of home institution
Terms of useLicence In copyright icon
Created on2017-05-03 08:04:08