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master's thesis
Terapijski potencijal moždanog neurotrofnog čimbenika, neurosteroida dehidroepiandrosterona i dehidroepiandrosteron sulfata u in vitro modelu Parkinsonove bolesti
Zagreb: University of Zagreb, Faculty of Science, 2022. urn:nbn:hr:217:815388
Bacelj, Nora
University of Zagreb
Faculty of Science
Department of Biology

Institutional repository: Repository of the Faculty of Science

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Bacelj, N. (2022). Terapijski potencijal moždanog neurotrofnog čimbenika, neurosteroida dehidroepiandrosterona i dehidroepiandrosteron sulfata u in vitro modelu Parkinsonove bolesti (Master's thesis). Zagreb: University of Zagreb, Faculty of Science. Retrieved from https://urn.nsk.hr/urn:nbn:hr:217:815388

Bacelj, Nora. "Terapijski potencijal moždanog neurotrofnog čimbenika, neurosteroida dehidroepiandrosterona i dehidroepiandrosteron sulfata u in vitro modelu Parkinsonove bolesti." Master's thesis, University of Zagreb, Faculty of Science, 2022. https://urn.nsk.hr/urn:nbn:hr:217:815388

Bacelj, Nora. "Terapijski potencijal moždanog neurotrofnog čimbenika, neurosteroida dehidroepiandrosterona i dehidroepiandrosteron sulfata u in vitro modelu Parkinsonove bolesti." Master's thesis, University of Zagreb, Faculty of Science, 2022. https://urn.nsk.hr/urn:nbn:hr:217:815388

Bacelj, N. (2022). 'Terapijski potencijal moždanog neurotrofnog čimbenika, neurosteroida dehidroepiandrosterona i dehidroepiandrosteron sulfata u in vitro modelu Parkinsonove bolesti', Master's thesis, University of Zagreb, Faculty of Science, accessed 22 November 2024, https://urn.nsk.hr/urn:nbn:hr:217:815388

Bacelj N. Terapijski potencijal moždanog neurotrofnog čimbenika, neurosteroida dehidroepiandrosterona i dehidroepiandrosteron sulfata u in vitro modelu Parkinsonove bolesti [Master's thesis]. Zagreb: University of Zagreb, Faculty of Science; 2022 [cited 2024 November 22] Available at: https://urn.nsk.hr/urn:nbn:hr:217:815388

N. Bacelj, "Terapijski potencijal moždanog neurotrofnog čimbenika, neurosteroida dehidroepiandrosterona i dehidroepiandrosteron sulfata u in vitro modelu Parkinsonove bolesti", Master's thesis, University of Zagreb, Faculty of Science, Zagreb, 2022. Available at: https://urn.nsk.hr/urn:nbn:hr:217:815388

Metadata
TitleTerapijski potencijal moždanog neurotrofnog čimbenika, neurosteroida dehidroepiandrosterona i dehidroepiandrosteron sulfata u in vitro modelu Parkinsonove bolesti
Title (english)Therapeutic potential of brain-derived neurotrophic factor, neurosteroid dehydroepiandrosterone and dehydroepiandrosterone sulfate in an in vitro model of Parkinson's disease
AuthorNora Bacelj
MentorMatea Nikolac Perković (mentor)
MentorJulija Erhardt (komentor)
Committee memberJulija Erhardt (predsjednik povjerenstva)
Committee memberDubravka Hranilović (član povjerenstva)
Committee memberZorana Grubić (član povjerenstva)
Committee memberSofia Ana Blažević (član povjerenstva)
GranterUniversity of Zagreb
Faculty of Science
(Department of Biology)
Zagreb
Defense date and country2022-09-21, Croatia
Scientific / art field,
discipline and subdiscipline		NATURAL SCIENCES
Biology
Abstract
Parkinsonova bolest je najčešći neurodegenerativni poremećaj kretanja kojeg karakteriziraju progresija bolesti te kompleksna klinička slika s motoričkim i ne-motoričkim simptomima. Neurosteroidi dehidroepiandrosteron (DHEA) i dehidroepiandrosteron sulfat (DHEAS) te moždani neurotrofni čimbenik (BDNF) ključni su za neuroprotekciju, neurogenezu, memoriju i učenje te djeluju antioksidativno i protuupalno. Uzimajući u obzir ove činjenice, kao i činjenicu da trenutno ne postoji lijek za Parkinsonovu bolest, ističe se važnost istraživanja novih potencijalnih terapeutika poput DHEA, DHEAS i BDNF koji će liječenje učiniti učinkovitijim. Cilj ovog rada je istražiti potencijalne učinke DHEA, DHEAS i BDNF u prevenciji smrti dopaminergičkih neurona uporabom in vitro modela, odnosno staničnih linija ozlijeđenih neurotoksinima rotenonom i 6-hidroksidopaminom koji uzrokuju oštećenja stanica nalik onima u Parkinsonovoj bolesti. U tu svrhu, provedene su analize vijabilnosti stanica, parametara oksidativnog stresa te detekcija stanične smrti. Rezultati istraživanja upućuju na to da DHEA, DHEAS i BDNF pokazuju značajan neuroprotektivni potencijal što se očituje kroz povećanje preživljavanja stanica te smanjenje razine unutarstaničnih reaktivnih kisikovih jedinki i apoptotičnih, odnosno nekrotičnih stanica.
Abstract (english)
Parkinson's disease is the most common neurodegenerative movement disorder characterized by disease progression and a complex clinical picture with motor and non-motor symptoms. The neurosteroids dehydroepiandrosterone (DHEA) and dehydroepiandrosterone sulfate (DHEAS) and brain-derived neurotrophic factor (BDNF) are crucial for neuroprotection, neurogenesis, memory and learning, and have antioxidant and anti-inflammatory effects. Considering these facts, as well as the fact that there is currently no cure for Parkinson's disease, the importance of research for new potential therapeutics such as DHEA, DHEAS and BDNF that will make the treatment more effective is essential. The aim of this thesis is to investigate the potential effects of DHEA, DHEAS and BDNF in preventing the death of dopaminergic neurons using in vitro model, i.e. cell lines injured by the neurotoxins rotenone and 6- hydroxydopamine, which cause cell damage similar to those in Parkinson's disease. For this purpose, analyzes of cell viability, oxidative stress parameters and detection of cell death were carried out. Research results indicate that DHEA, DHEAS and BDNF show a significant neuroprotective potential, which is manifested through an increase in cell survival and a decrease in the level of intracellular reactive oxygen species and apoptotic and/or necrotic cells.
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:217:815388
Study programmeTitle: Experimental Biology
Study programme type: university
Study level: graduate
Academic / professional title: magistar/magistra eksperimentalne biologije (magistar/magistra eksperimentalne biologije)
Type of resourceText
File originBorn digital
Access conditionsOpen access
Terms of useLicence In copyright icon
Created on2022-10-10 10:48:49