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undergraduate thesis
Usmjerena evolucija kao metoda u dizajnu novih katalitičkih aktivnosti enzima
Zagreb: University of Zagreb, Faculty of Science, 2022. urn:nbn:hr:217:279245
Zubčić, Ivan
University of Zagreb
Faculty of Science
Department of Biology
University of Zagreb
Faculty of Science
Department of Chemistry

Institutional repository: Repository of the Faculty of Science

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Zubčić, I. (2022). Usmjerena evolucija kao metoda u dizajnu novih katalitičkih aktivnosti enzima (Undergraduate thesis). Zagreb: University of Zagreb, Faculty of Science; Zagreb: University of Zagreb, Faculty of Science. Retrieved from https://urn.nsk.hr/urn:nbn:hr:217:279245

Zubčić, Ivan. "Usmjerena evolucija kao metoda u dizajnu novih katalitičkih aktivnosti enzima." Undergraduate thesis, University of Zagreb, Faculty of Science; University of Zagreb, Faculty of Science, 2022. https://urn.nsk.hr/urn:nbn:hr:217:279245

Zubčić, Ivan. "Usmjerena evolucija kao metoda u dizajnu novih katalitičkih aktivnosti enzima." Undergraduate thesis, University of Zagreb, Faculty of Science; University of Zagreb, Faculty of Science, 2022. https://urn.nsk.hr/urn:nbn:hr:217:279245

Zubčić, I. (2022). 'Usmjerena evolucija kao metoda u dizajnu novih katalitičkih aktivnosti enzima', Undergraduate thesis, University of Zagreb, Faculty of Science; University of Zagreb, Faculty of Science, accessed 25 November 2024, https://urn.nsk.hr/urn:nbn:hr:217:279245

Zubčić I. Usmjerena evolucija kao metoda u dizajnu novih katalitičkih aktivnosti enzima [Undergraduate thesis]. Zagreb: University of Zagreb, Faculty of Science; Zagreb: University of Zagreb, Faculty of Science; 2022 [cited 2024 November 25] Available at: https://urn.nsk.hr/urn:nbn:hr:217:279245

I. Zubčić, "Usmjerena evolucija kao metoda u dizajnu novih katalitičkih aktivnosti enzima", Undergraduate thesis, University of Zagreb, Faculty of Science, Zagreb; University of Zagreb, Faculty of Science, Zagreb, 2022. Available at: https://urn.nsk.hr/urn:nbn:hr:217:279245

Metadata
TitleUsmjerena evolucija kao metoda u dizajnu novih katalitičkih aktivnosti enzima
Title (english)Directed evolution as a method in the design of new catalytic activities of enzymes
AuthorIvan Zubčić
MentorIta Gruić-Sovulj (mentor)
GranterUniversity of Zagreb
Faculty of Science
(Department of Biology)
Zagreb
GranterUniversity of Zagreb
Faculty of Science
(Department of Chemistry)
Zagreb
Defense date and country2022-09-22, Croatia
Scientific / art field,
discipline and subdiscipline		NATURAL SCIENCES
Biology
Abstract
Usmjerena evolucija je relativno nova metoda u dizajniranju već postojećih proteina. Metoda se temelji na uvođenju promjena u slijedu nukleotida DNA koja kodira za željeni protein. Nakon uvođenja promjena u željeni slijed nukleotida, pokušava se nekom metodom selekcije ili probira identificirati protein s promjenjenim svojstvima koja mogu sezati od od afiniteta eznima prema supstratu do termostabilnosti samog proteina. Važno je konstruirati pogodan sustav za probir ili selekciju koji omogćuje pronalaženje modificiranog proteina. Usmjerena evolucija je omogućila modifikaciju proteina u vidu kataliziranja novih reakcija, kataliziranju nastanka novih kemijskih veza, sintezi određenih molekula poput biogoriva i mnogih drugih mogućnosti.
Abstract (english)
Directed evolution is a relatively new method in designing already existing proteins. The method is based on the introduction of a change in the DNA sequence that codes for the desired protein. After the DNA sequence has been altered, an attempt is made by some method of selection to try to identify a protein with changed properties ranging from the affinity of enzymes to the substrate to the thermostability of the protein itself . It is important to construct a suitable screening/selection system that allows finding the modified protein. Directed evolution has made it possible to modify proteins in a way of catalyzing new reactions, catalyzing the formation of chemical bonds, synthesizing certain molecules such as biofuels and many other possibilities.
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:217:279245
Study programmeTitle: Molecular Biology
Study programme type: university
Study level: undergraduate
Academic / professional title: sveučilišni/a prvostupnik/prvostupnica (baccalaureus/baccalaurea) molekularne biologije (sveučilišni/a prvostupnik/prvostupnica (baccalaureus/baccalaurea) molekularne biologije)
Type of resourceText
File originBorn digital
Access conditionsOpen access
Terms of useLicence In copyright icon
Created on2022-10-13 10:41:54