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master's thesis
Krivulje rasta bakterija Bacillus subtilis i Acinetobacter baumannii u ovisnosti o temperaturi
Zagreb: University of Zagreb, Faculty of Science, 2022. urn:nbn:hr:217:046598
Ćelić, Branimira
University of Zagreb
Faculty of Science
Department of Biology

Institutional repository: Repository of the Faculty of Science

Cite this document

Ćelić, B. (2022). Krivulje rasta bakterija Bacillus subtilis i Acinetobacter baumannii u ovisnosti o temperaturi (Master's thesis). Zagreb: University of Zagreb, Faculty of Science. Retrieved from https://urn.nsk.hr/urn:nbn:hr:217:046598

Ćelić, Branimira. "Krivulje rasta bakterija Bacillus subtilis i Acinetobacter baumannii u ovisnosti o temperaturi." Master's thesis, University of Zagreb, Faculty of Science, 2022. https://urn.nsk.hr/urn:nbn:hr:217:046598

Ćelić, Branimira. "Krivulje rasta bakterija Bacillus subtilis i Acinetobacter baumannii u ovisnosti o temperaturi." Master's thesis, University of Zagreb, Faculty of Science, 2022. https://urn.nsk.hr/urn:nbn:hr:217:046598

Ćelić, B. (2022). 'Krivulje rasta bakterija Bacillus subtilis i Acinetobacter baumannii u ovisnosti o temperaturi', Master's thesis, University of Zagreb, Faculty of Science, accessed 22 November 2024, https://urn.nsk.hr/urn:nbn:hr:217:046598

Ćelić B. Krivulje rasta bakterija Bacillus subtilis i Acinetobacter baumannii u ovisnosti o temperaturi [Master's thesis]. Zagreb: University of Zagreb, Faculty of Science; 2022 [cited 2024 November 22] Available at: https://urn.nsk.hr/urn:nbn:hr:217:046598

B. Ćelić, "Krivulje rasta bakterija Bacillus subtilis i Acinetobacter baumannii u ovisnosti o temperaturi", Master's thesis, University of Zagreb, Faculty of Science, Zagreb, 2022. Available at: https://urn.nsk.hr/urn:nbn:hr:217:046598

Metadata
TitleKrivulje rasta bakterija Bacillus subtilis i Acinetobacter baumannii u ovisnosti o temperaturi
Title (english)Temperature dependent growth curves of bacteria Bacillus subtilis and Acinetobacter baumannii
AuthorBranimira Ćelić
MentorJasna Hrenović (mentor)
Committee memberJasna Hrenović (predsjednik povjerenstva)
Committee memberRenata Šoštarić (član povjerenstva)
Committee memberBiljana Balen (član povjerenstva)
Committee memberPetar Kružić (član povjerenstva)
GranterUniversity of Zagreb
Faculty of Science
(Department of Biology)
Zagreb
Defense date and country2022-09-23, Croatia
Scientific / art field,
discipline and subdiscipline		NATURAL SCIENCES
Biology
Abstract
Bacillus subtilis je Gram-pozitivna bakterija štapićastog oblika koja stvara spore otporne na visoku temperaturu. Nalazi se u tlu i nepatogena je. Bakterija Acinetobacter baumannii je Gramnegativni kokobacil koji se najčešće pojavljuje u bolničkom okruženju. Cilj ovog istraživanja bio je utvrditi krivulje rasta, preživljavanje i eventualno umnažanje bakterija B. subtilis i A. baumannii, kao i koncentraciju endospora B. subtilis u ovisnosti o različitim temperaturama inkubacije (4, 22, 35, 44 i 50 °C) kroz 52 h u hranjivom mediju. Obje bakterije su preživjele sve istraživane temperaturne vrijednosti. Uočen je izostanak umnažanja obje bakterije pri temperaturi od 4 °C. Što je temperatura inkubacije bila bliža temperaturnom optimumu bakterijskih vrsta, to je i umnažanje bilo značajnije. Udio endospora u kulturi B. subtilis se izrazito povećao nakon 5 h inkubacije pri 50 °C kao način prilagodbe na nepovoljne uvjete okoliša.
Abstract (english)
Bacillus subtilis is a Gram-positive, rod-shaped bacterium that forms spores resistant to high temperatures. It is found in the soil and is non-pathogenic. The bacterium Acinetobacter baumannii is a Gram-negative coccobacillus that occurs most often in the hospital environment. The aim of this research was to determine the growth curves, survival, and eventual reproduction of B. subtilis and A. baumannii, as well as the concentration of B. subtilis endospores depending on different incubation temperatures (4, 22, 35, 44 and 50 °C) over 52 hours. Both bacteria survived all investigated temperature ranges. The absence of multiplication of both bacteria at a temperature of 4 °C was observed. The closer the incubation temperature was to the temperature optimum of the bacterial species, the more significant the multiplication was. The proportion of endospores in the culture of B. subtilis increased after 5 hours of incubation at 50 °C as a way of adapting to unfavorable environmental conditions.
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:217:046598
Study programmeTitle: Experimental Biology
Study programme type: university
Study level: graduate
Academic / professional title: magistar/magistra eksperimentalne biologije (magistar/magistra eksperimentalne biologije)
Type of resourceText
File originBorn digital
Access conditionsOpen access
Terms of useLicence In copyright icon
Created on2022-10-17 08:02:46