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master's thesis
Utjecaj akutne neonatalne hipoksije na ekspresiju gena za vazoaktivni intestinalni peptid u hipokampusu adolescentnih štakora
Zagreb: University of Zagreb, Faculty of Science, 2022. urn:nbn:hr:217:992252
Runko, Valentina
University of Zagreb
Faculty of Science

Institutional repository: Repository of the Faculty of Science

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Runko, V. (2022). Utjecaj akutne neonatalne hipoksije na ekspresiju gena za vazoaktivni intestinalni peptid u hipokampusu adolescentnih štakora (Master's thesis). Zagreb: University of Zagreb, Faculty of Science. Retrieved from https://urn.nsk.hr/urn:nbn:hr:217:992252

Runko, Valentina. "Utjecaj akutne neonatalne hipoksije na ekspresiju gena za vazoaktivni intestinalni peptid u hipokampusu adolescentnih štakora." Master's thesis, University of Zagreb, Faculty of Science, 2022. https://urn.nsk.hr/urn:nbn:hr:217:992252

Runko, Valentina. "Utjecaj akutne neonatalne hipoksije na ekspresiju gena za vazoaktivni intestinalni peptid u hipokampusu adolescentnih štakora." Master's thesis, University of Zagreb, Faculty of Science, 2022. https://urn.nsk.hr/urn:nbn:hr:217:992252

Runko, V. (2022). 'Utjecaj akutne neonatalne hipoksije na ekspresiju gena za vazoaktivni intestinalni peptid u hipokampusu adolescentnih štakora', Master's thesis, University of Zagreb, Faculty of Science, accessed 25 November 2024, https://urn.nsk.hr/urn:nbn:hr:217:992252

Runko V. Utjecaj akutne neonatalne hipoksije na ekspresiju gena za vazoaktivni intestinalni peptid u hipokampusu adolescentnih štakora [Master's thesis]. Zagreb: University of Zagreb, Faculty of Science; 2022 [cited 2024 November 25] Available at: https://urn.nsk.hr/urn:nbn:hr:217:992252

V. Runko, "Utjecaj akutne neonatalne hipoksije na ekspresiju gena za vazoaktivni intestinalni peptid u hipokampusu adolescentnih štakora", Master's thesis, University of Zagreb, Faculty of Science, Zagreb, 2022. Available at: https://urn.nsk.hr/urn:nbn:hr:217:992252

Metadata
TitleUtjecaj akutne neonatalne hipoksije na ekspresiju gena za vazoaktivni intestinalni peptid u hipokampusu adolescentnih štakora
Title (english)The effect of acute neonatal hypoxia on the expression of vasoactive intestinal polypeptide gene in adolescent rat hippocampus
AuthorValentina Runko
MentorDubravka Hranilović (mentor)
Committee memberDubravka Hranilović (predsjednik povjerenstva)
Committee memberSilvija Černi (član povjerenstva)
Committee memberDuje Lisičić (član povjerenstva)
Committee memberDubravko Pavoković (član povjerenstva)
GranterUniversity of Zagreb
Faculty of Science
Zagreb
Defense date and country2022-04-28, Croatia
Scientific / art field,
discipline and subdiscipline		NATURAL SCIENCES
Biology
Abstract
Vazoaktivni intestinalni peptid (VIP) je regulatorna molekula, koja u sisavaca ima ulogu neurotransmitera, hormona te faktora rasta i razvoja. Unutar hipokampusa, VIP sudjeluje u kontroli sinaptičke plastičnosti, poput dugotrajne potencijacije i dugotrajne depresije. Smatra se da neuronski krugovi koji eksprimiraju VIP moderiraju prostorno učenje potaknuto nagradom. Ovisno o stupnju i trajanju, prenatalna hipoksija u ljudi spada u faktore rizika za niz poremećaja, uključujući cerebralnu paralizu, poremećaj pažnje s hiperaktivnošću, poremećaje autističnog spektra te poteškoće u prostornom učenju i radnoj memoriji. U ovom smo radu, koristeći model akutne neonatalne hipoksije u normobaričnim uvjetima, istražili uzrokuje li neonatalno izlaganje štakora hipoksiji promjenu u razini ekspresije gena za VIP u hipokampusu adolescenth štakora, kod kojih je ranije opažena promjena u prostornom učenju. Diferencijalnu ekspresiju gena za VIP istražila sam kvantitativnom lančanom reakcijom polimerazom, korištenjem RNA prethodno izolirane iz hipokampusa 16 tretiranih i 15 kontrolnih štakora oba spola. Dvosmjerna analiza varijanci pokazala je značajan utjecaj tretmana na ekspresiju gena za VIP, uz 2,5 puta veću ekspresiju u hipoksičnoj skupini. Zaključujemo da je akutno neonatalno izlaganje hipoksiji moglo dovesti do dugotrajnog kompenzatornog povećanja ekspresije gena za VIP, koje se pak može povezati s promjenama u prostornom učenju posredovanom nagradom.
Abstract (english)
Vasoactive intestinal peptide (VIP) is a regulatory molecule in the mammalian brain, acting as a neurotransmitter, hormone, and growth factor. Within the hippocampus, VIP participates in controlling synaptic plasticity phenomena, such as long-term potentiation and long-term depression. Neuron circuits which express VIP are thought to moderate reward-associated learning. Depending on duration and severity, prenatal and neonatal hypoxia is a risk factor for various disorders, including cerebral palsy, attention-deficit hyperactive disorder, autism spectrum disorders, and difficulties in spatial learning and working memory. In this paper, we used a model of neonatal hypoxia in normobaric conditions, in order to explore whether neonatal exposure to hypoxia changes VIP expression in the hippocampus of adolescent rats, which were earlier shown to have altered spatial learning. In order to explore the differential expression of VIP gene, I used qPCR method on RNA isolated from the hippocampus of adolescent rats of both sexes, 16 of which were subjected to hypoxia treatment, and 15 of which were controls. Two-way ANOVA showed a significant influence of treatment on VIP gene expression, which was 2.5 times higher in the hypoxia treated group. We conclude that acute neonatal exposure to hypoxia may lead to a long-term compensatory increase in VIP gene expression, which can be correlated to changes in reward-motivated spatial learning.
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:217:992252
ProjectNumber: IP-2019-04-3182
Title: Moždani izvanstanični matriks u razvitku i perinatalnoj hipoksiji
Title: Brain extracellular matrix in development and in perinatal hypoxia
Acronym: BRAINECM
Leader: Nataša Jovanov Milošević
Jurisdiction: Croatia
Funder: HRZZ
Funding stream: IP
ProjectNumber: KK.01.1.1.01.0007
Title: Eksperimentalna i klinička istraživanja hipoksijsko-ishemijskog oštećenja mozga u perinatalnoj i odrasloj dobi
Leader: Miloš Judaš
Jurisdiction: Croatia
Funder: ERDF
Study programmeTitle: Experimental Biology
Study programme type: university
Study level: graduate
Academic / professional title: magistar/magistra eksperimentalne biologije (magistar/magistra eksperimentalne biologije)
Type of resourceText
File originBorn digital
Access conditionsOpen access
Embargo expiration date: 2023-04-29
Terms of useLicence In copyright icon
Created on2022-10-17 08:55:16