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master's thesis
Unutarstanično prometovanje čvrstih lipidnih nanočestica
Zagreb: University of Zagreb, Faculty of Science, 2011. urn:nbn:hr:217:051287
Herceg, Viktorija
University of Zagreb
Faculty of Science
Department of Biology

Institutional repository: Repository of the Faculty of Science

Cite this document

Herceg, V. (2011). Unutarstanično prometovanje čvrstih lipidnih nanočestica (Master's thesis). Zagreb: University of Zagreb, Faculty of Science. Retrieved from https://urn.nsk.hr/urn:nbn:hr:217:051287

Herceg, Viktorija. "Unutarstanično prometovanje čvrstih lipidnih nanočestica." Master's thesis, University of Zagreb, Faculty of Science, 2011. https://urn.nsk.hr/urn:nbn:hr:217:051287

Herceg, Viktorija. "Unutarstanično prometovanje čvrstih lipidnih nanočestica." Master's thesis, University of Zagreb, Faculty of Science, 2011. https://urn.nsk.hr/urn:nbn:hr:217:051287

Herceg, V. (2011). 'Unutarstanično prometovanje čvrstih lipidnih nanočestica', Master's thesis, University of Zagreb, Faculty of Science, accessed 22 December 2024, https://urn.nsk.hr/urn:nbn:hr:217:051287

Herceg V. Unutarstanično prometovanje čvrstih lipidnih nanočestica [Master's thesis]. Zagreb: University of Zagreb, Faculty of Science; 2011 [cited 2024 December 22] Available at: https://urn.nsk.hr/urn:nbn:hr:217:051287

V. Herceg, "Unutarstanično prometovanje čvrstih lipidnih nanočestica", Master's thesis, University of Zagreb, Faculty of Science, Zagreb, 2011. Available at: https://urn.nsk.hr/urn:nbn:hr:217:051287

Metadata
TitleUnutarstanično prometovanje čvrstih lipidnih nanočestica
Title (english)Intracellular trafficking of solid lipid nanoparticles
AuthorViktorija Herceg
MentorMaja Matulić (mentor)
Committee memberMaja Matulić (predsjednik povjerenstva)
Committee memberAna Galov (član povjerenstva)
Committee memberInga Marijanović (član povjerenstva)
GranterUniversity of Zagreb
Faculty of Science
(Department of Biology)
Zagreb
Defense date and country2011-10-21, Croatia
Scientific / art field,
discipline and subdiscipline		NATURAL SCIENCES
Biology
Abstract
Čvrste lipidne nanočestice (SLN) su koloidalni prenosioci lijekova izgrađeni od biorazgradivih lipida. One poboljšavaju bioraspoloživost molekula slabo topivih u vodi i omogućuju produženo otpuštanje lijeka. SLN punjene edelfosinom, lijekom protiv raka, pokazale su obećavajuće rezultate u životinjskim modelima in vivo. Međutim, mehanizam djelovanja edelfosina ostao je nepoznat. Ciljevi ovoga rada bili su: razjasniti unos i unutarstanično prometovanje SLN fluorescencijskom mikroskopijom i transmisijskom elektronskom mikroskopijom (TEM) te procijeniti djelovanje slobodnog edelfosina i SLN punjenih edelfosinom na stanične linije raka inhibicijom proliferacije i apoptozom. SLN su bile pripremljene vrućom homogenizacijom. Razni fluorescentni spojevi (perilen, DiD, rodamin i Nile Red) bili su uspješno uklopljeni u SLN. Rezultati fluorescencijske mikroskopije i TEM-a pokazali su da su SLN ušle u stanice HL-60, K562 i GS-9L. Otpornost stanica GS-9L na djelovanje slobodnog edelfosina pokazana je inhibicijom proliferacije. SLN punjene edelfosinom djelovale su na stanice GS-9L, dok sam lijek nije pokazao nikakav učinak.
Abstract (english)
Solid lipid nanoparticlers (SLN) are colloidal drug carriers made of biodegradable lipids. They can improve the bioavailability of poorly water-soluble molecules and provide sustained release over time. SLN loaded with the anti-cancer drug edelfosine showed promising results in in vivo xenograft animal models. However, edelfosine mechanism of action remains unknown. The objectives were: to elucidate the uptake and intracellular trafficking of SLN by using fluorescent microscopy and transmission electron microscopy (TEM) and to evaluate the effect of free edelfosine and edelfosine-loaded SLN on cancer cell lines by inhibition of proliferation and apoptosis. SLN were prepared by hot homogenization. Various fluorescent compounds (Perylene, DiD, Rhodamine, Nile Red) were successfully encapsulated in SLN. SLN were uptaken by HL-60, K562 and GS-9L cells as demonstrated by fluorescent and TEM studies. GS-9L cell were resistant to action of free edelfosine as demonstrated by inhibition of proliferation. Edelfosine loaded-SLN were effective in treating GS-9L cell line whereas the free-drug was practically devoid of efficacy.
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:217:051287
Study programmeTitle: Molecular Biology
Study programme type: university
Study level: graduate
Academic / professional title: magistar/magistra molekularne biologije (magistar/magistra molekularne biologije)
Type of resourceText
File originBorn digital
Access conditionsAccess restricted to students and staff of home institution
Terms of useLicence In copyright icon
Created on2017-05-03 08:29:17