prikaz prve stranice dokumenta Dinamika specijaliziranoga metabolizma i fiziološkog odgovora vinove loze (Vitis vinifera) inficirane fitoplazmom flavescence dorée
Embargo period 2025-09-26
master's thesis
Dinamika specijaliziranoga metabolizma i fiziološkog odgovora vinove loze (Vitis vinifera) inficirane fitoplazmom flavescence dorée
Zagreb: University of Zagreb, Faculty of Science, 2022. urn:nbn:hr:217:743644
Davosir, Dino
University of Zagreb
Faculty of Science
Department of Biology

Institutional repository: Repository of the Faculty of Science

Cite this document

Davosir, D. (2022). Dinamika specijaliziranoga metabolizma i fiziološkog odgovora vinove loze (Vitis vinifera) inficirane fitoplazmom flavescence dorée (Master's thesis). Zagreb: University of Zagreb, Faculty of Science. Retrieved from https://urn.nsk.hr/urn:nbn:hr:217:743644

Davosir, Dino. "Dinamika specijaliziranoga metabolizma i fiziološkog odgovora vinove loze (Vitis vinifera) inficirane fitoplazmom flavescence dorée." Master's thesis, University of Zagreb, Faculty of Science, 2022. https://urn.nsk.hr/urn:nbn:hr:217:743644

Davosir, Dino. "Dinamika specijaliziranoga metabolizma i fiziološkog odgovora vinove loze (Vitis vinifera) inficirane fitoplazmom flavescence dorée." Master's thesis, University of Zagreb, Faculty of Science, 2022. https://urn.nsk.hr/urn:nbn:hr:217:743644

Davosir, D. (2022). 'Dinamika specijaliziranoga metabolizma i fiziološkog odgovora vinove loze (Vitis vinifera) inficirane fitoplazmom flavescence dorée', Master's thesis, University of Zagreb, Faculty of Science, accessed 21 May 2024, https://urn.nsk.hr/urn:nbn:hr:217:743644

Davosir D. Dinamika specijaliziranoga metabolizma i fiziološkog odgovora vinove loze (Vitis vinifera) inficirane fitoplazmom flavescence dorée [Master's thesis]. Zagreb: University of Zagreb, Faculty of Science; 2022 [cited 2024 May 21] Available at: https://urn.nsk.hr/urn:nbn:hr:217:743644

D. Davosir, "Dinamika specijaliziranoga metabolizma i fiziološkog odgovora vinove loze (Vitis vinifera) inficirane fitoplazmom flavescence dorée", Master's thesis, University of Zagreb, Faculty of Science, Zagreb, 2022. Available at: https://urn.nsk.hr/urn:nbn:hr:217:743644

Metadata
TitleDinamika specijaliziranoga metabolizma i fiziološkog odgovora vinove loze (Vitis vinifera) inficirane fitoplazmom flavescence dorée
Title (english)Dynamics of specialized metabolism and physiological responses of grapevine (Vitis vinifera) infected with flavescence dorée phytoplasma
AuthorDino Davosir
MentorMartina Šeruga Musić (mentor)
MentorIvana Šola (komentor)
Committee memberMartina Šeruga Musić (predsjednik povjerenstva)
Committee memberBiljana Balen (član povjerenstva)
Committee memberAna Galov (član povjerenstva)
Committee memberMarin Ježić (član povjerenstva)
GranterUniversity of Zagreb
Faculty of Science
(Department of Biology)
Zagreb
Defense date and country2022-09-26, Croatia
Scientific / art field,
discipline and subdiscipline		NATURAL SCIENCES
Biology
Abstract
Fitoplazme (rod ‘Candidatus Phytoplasma’) su unutarstanične parazitske bakterije i patogeni širokog kruga biljaka. Fitoplazma flavescence dorée (FDp) uzročnik je velikih šteta u vinogradarskoj proizvodnji u Europi. U ovom istraživanju, sakupljeni su listovi simptomatskih i asimptomatskih listova vinove loze (Vitis vinifera L.) var. 'Pinot gris' u tri vremenske točke tijekom razvoja infekcije. FDp je detektirana korištenjem kvantitativnog PCR i genotipizirana sekvenciranjem i filogenetskom analizom map gena, što je pokazalo prisutnost dva genotipa, M38 i M54. Korištenjem spektrofotometrijskih i kromatografskih (RP-HPLC, GC-MS) metoda, analiziran je utjecaj dva FDp genotipa na fiziološke parametre i fenolni metabolizam vinove loze tijekom razvoja infekcije. Genotip M54 je slabije djelovao na fiziološke parametre, ali je održavao viši titar nego M38. Analiza korelacija pokazala je da promjene fizioloških parametara mogu biti povezane s titrom FDp. Sadržaj svih glavnih grana fenolnog metabolizma i individualnih spojeva je pokazao slične obrasce, ukazujući na pojačanje temeljnog fenolnog biosintetskog puta zbog infekcije FDp, posebno u trećoj vremenskoj točki. Analizirane promjene fiziologije vinove loze ovisne o genotipu FDp daju podatke o metama FDp u vinovoj lozi i mogu poduprijeti identifikaciju potencijalnih efektora ove fitoplazme.
Abstract (english)
Phytoplasmas (genus ‘Candidatus Phytoplasma’) are intracellular parasitic bacteria and pathogens of numerous plants. Flavescence dorée phytoplasma (FDp) causes severe damage to viticultural production in Europe and is a quarantine pathogen in EU. In this study, symptomatic and asymptomatic grapevine (Vitis vinifera L.) var. ‘Pinot gris’ leaves were sampled at three time points through the development of infection. FDp was detected by using triplex real-time PCR and genotyped by sequencing and phylogenetic analysis of map gene which identified two genotypes, M38 and M54. By using spectrophotometric and chromatographic (RP-HPLC, GC-MS) analyses, impact of two FDp genotypes on grapevine physiological parameters and phenolic metabolism was evaluated through the development of infection. Genotype M54 impacted physiological parameters less severely, while maintaining higher titre than M38. Correlation analysis revealed that changes in physiological parameters could be linked to FDp titre. Content of all main branches of phenolic metabolism and most individual compounds had similar trends, pointing to the general induction of the stem phenolic biosynthetic pathway by FDp infection, particularly in the last time point. Analysed phytoplasma genotype-dependant changes to grapevine physiology provide data on certain targets of FDp in grapevine and could assist the identification of potential specific effectors of this phytoplasma.
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:217:743644
Study programmeTitle: Molecular Biology
Study programme type: university
Study level: graduate
Academic / professional title: magistar/magistra molekularne biologije (magistar/magistra molekularne biologije)
Type of resourceText
File originBorn digital
Access conditionsEmbargoed access
Embargo expiration date: 2025-09-26
Terms of useLicence In copyright icon
Created on2022-10-20 12:30:34