prikaz prve stranice dokumenta Ekspresija gena potaknuta signalizacijom Toll-like receptora 2 u kroničnoj fazi oporavka nakon ishemijske ozljede mišjeg mozga
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master's thesis
Ekspresija gena potaknuta signalizacijom Toll-like receptora 2 u kroničnoj fazi oporavka nakon ishemijske ozljede mišjeg mozga
Zagreb: University of Zagreb, Faculty of Science, 2011. urn:nbn:hr:217:616858
Krmpotić, Lora
University of Zagreb
Faculty of Science
Department of Biology

Institutional repository: Repository of the Faculty of Science

Cite this document

Krmpotić, L. (2011). Ekspresija gena potaknuta signalizacijom Toll-like receptora 2 u kroničnoj fazi oporavka nakon ishemijske ozljede mišjeg mozga (Master's thesis). Zagreb: University of Zagreb, Faculty of Science. Retrieved from https://urn.nsk.hr/urn:nbn:hr:217:616858

Krmpotić, Lora. "Ekspresija gena potaknuta signalizacijom Toll-like receptora 2 u kroničnoj fazi oporavka nakon ishemijske ozljede mišjeg mozga." Master's thesis, University of Zagreb, Faculty of Science, 2011. https://urn.nsk.hr/urn:nbn:hr:217:616858

Krmpotić, Lora. "Ekspresija gena potaknuta signalizacijom Toll-like receptora 2 u kroničnoj fazi oporavka nakon ishemijske ozljede mišjeg mozga." Master's thesis, University of Zagreb, Faculty of Science, 2011. https://urn.nsk.hr/urn:nbn:hr:217:616858

Krmpotić, L. (2011). 'Ekspresija gena potaknuta signalizacijom Toll-like receptora 2 u kroničnoj fazi oporavka nakon ishemijske ozljede mišjeg mozga', Master's thesis, University of Zagreb, Faculty of Science, accessed 26 November 2024, https://urn.nsk.hr/urn:nbn:hr:217:616858

Krmpotić L. Ekspresija gena potaknuta signalizacijom Toll-like receptora 2 u kroničnoj fazi oporavka nakon ishemijske ozljede mišjeg mozga [Master's thesis]. Zagreb: University of Zagreb, Faculty of Science; 2011 [cited 2024 November 26] Available at: https://urn.nsk.hr/urn:nbn:hr:217:616858

L. Krmpotić, "Ekspresija gena potaknuta signalizacijom Toll-like receptora 2 u kroničnoj fazi oporavka nakon ishemijske ozljede mišjeg mozga", Master's thesis, University of Zagreb, Faculty of Science, Zagreb, 2011. Available at: https://urn.nsk.hr/urn:nbn:hr:217:616858

Metadata
TitleEkspresija gena potaknuta signalizacijom Toll-like receptora 2 u kroničnoj fazi oporavka nakon ishemijske ozljede mišjeg mozga
Title (english)Expression of genes involved in TLR2 signaling pathway during the chronic phase of recovery after ischemia in mouse brain
AuthorLora Krmpotić
MentorSrećko Gajović (mentor)
Committee memberIvana Ivančić Baće (predsjednik povjerenstva)
Committee memberSrećko Gajović (član povjerenstva)
Committee memberJasna Lajtner (član povjerenstva)
GranterUniversity of Zagreb
Faculty of Science
(Department of Biology)
Zagreb
Defense date and country2011-07-11, Croatia
Scientific / art field,
discipline and subdiscipline		NATURAL SCIENCES
Biology
Abstract
Moždani udar godišnje pogodi 15 milijuna ljudi diljem svijeta i vodeći je uzrok invaliditeta u odraslih. Ishemijski moždani udar nastaje začepljenjem arterije u mozgu, a kao posljedica nastaje hiperfuzija koja vodi ekscitotoksičnosti, oksidativnom stresu, disfunkciji krvno-moždane barijere, te aktivaciji post-ishemijske upale. Cilj ovog rada bio je pokazati kako se ekspresija gena koji sudjeluju u TLR2 signalnom putu mijenja u kroničnoj fazi reperfuzije vremenskog razdoblja od 72h do 1 mjesec. Miševima je začepljena središnja moždana arterija tijekom 1 sata, izoliran im je mozak i izmjerena razina ekspresije gena kvantitativnom lančanom reakcijom polimeraze. Rad je pokazao da se ekspresija gena aktivatora TLR2 signalnog puta smanjila, a inhibitora povećala u TLR2 KO miševa u odnosu na divlji tip. Regulacija gena u TLR2 signalizaciji mogla bi biti ključ za ublažavanje štete nastale ishemijskim moždanim udarom.
Abstract (english)
15 million people a year worldwide are afflected with stroke which is the main cause of disability in adults. Ischemic stroke is caused by blockage of blood flow in the brain, which subsequently causes hyperfusion, excitotoxicity, oxydative stress, damage of blood-brain barrier and postischemic inflammation. The aim of the thesis was to show how the expression of TLR2 signaling pathway genes changes during the chronic phase of recovery, 72 h to 1 month after induced ischemia in mouse brain. Middle cerebral artery occlusion (MCAO) was performed on mice for induction of ischemia and qPCR to measure the relative expression of selected genes. The results showed that the expression of pathway activators decreased and inhibitors increased in TLR2 KO relative to WT, in chronic phase of reperfusion. Regulation of these genes in TLR2 signaling pathway could prove to be paramount in attenuating damage caused by ischemic stroke.
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:217:616858
Study programmeTitle: Molecular Biology
Study programme type: university
Study level: graduate
Academic / professional title: magistar/magistra molekularne biologije (magistar/magistra molekularne biologije)
Type of resourceText
File originBorn digital
Access conditionsAccess restricted to students and staff of home institution
Terms of useLicence In copyright icon
Created on2017-05-03 09:08:17