prikaz prve stranice dokumenta The role of tumor endothelial marker 1 in lung metastasis
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master's thesis
The role of tumor endothelial marker 1 in lung metastasis
Zagreb: University of Zagreb, Faculty of Science, 2022. urn:nbn:hr:217:968120
Dragičević, Dorian
University of Zagreb
Faculty of Science
Department of Biology

Institutional repository: Repository of the Faculty of Science

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Dragičević, D. (2022). The role of tumor endothelial marker 1 in lung metastasis (Master's thesis). Zagreb: University of Zagreb, Faculty of Science. Retrieved from https://urn.nsk.hr/urn:nbn:hr:217:968120

Dragičević, Dorian. "The role of tumor endothelial marker 1 in lung metastasis." Master's thesis, University of Zagreb, Faculty of Science, 2022. https://urn.nsk.hr/urn:nbn:hr:217:968120

Dragičević, Dorian. "The role of tumor endothelial marker 1 in lung metastasis." Master's thesis, University of Zagreb, Faculty of Science, 2022. https://urn.nsk.hr/urn:nbn:hr:217:968120

Dragičević, D. (2022). 'The role of tumor endothelial marker 1 in lung metastasis', Master's thesis, University of Zagreb, Faculty of Science, accessed 22 November 2024, https://urn.nsk.hr/urn:nbn:hr:217:968120

Dragičević D. The role of tumor endothelial marker 1 in lung metastasis [Master's thesis]. Zagreb: University of Zagreb, Faculty of Science; 2022 [cited 2024 November 22] Available at: https://urn.nsk.hr/urn:nbn:hr:217:968120

D. Dragičević, "The role of tumor endothelial marker 1 in lung metastasis", Master's thesis, University of Zagreb, Faculty of Science, Zagreb, 2022. Available at: https://urn.nsk.hr/urn:nbn:hr:217:968120

Metadata
TitleThe role of tumor endothelial marker 1 in lung metastasis
Title (croatian)Uloga tumorskog endotelnog markera 1 u metastaziranju pluća
AuthorDorian Dragičević
MentorStefan Offermanns (mentor)
MentorPetra Korać (komentor)
Committee memberPetra Korać (predsjednik povjerenstva)
Committee memberInga Urlić (član povjerenstva)
Committee memberBiljana Balen (član povjerenstva)
Committee memberPetra Peharec Štefanić (član povjerenstva)
GranterUniversity of Zagreb
Faculty of Science
(Department of Biology)
Zagreb
Defense date and country2022-09-27, Croatia
Scientific / art field,
discipline and subdiscipline		NATURAL SCIENCES
Biology
Abstract
Cancer relapse is driven by dormant tumor cells that are able to reactivate after perturbations in the microenvironmental niche. Endothelium-derived protein multimerin 2 (MMRN2) is hypothesized to induce tumor reawakening through the interaction with tumor endothelial marker 1 (TEM1). The main objective of this study was to assess the MMRN2-TEM1 axis in reactivation and proliferation of B16F10 melanoma and E0771 breast cancer cells, and elucidate function of TEM1 in lung metastasis. The MMRN2-TEM1 axis, induced by a coculture system comprised of endothelial cells and tumor cells, has demonstrated to stimulate phosphorylation of ERK1/2. Individual addition of PDGFB and MMRN2 fragments to dormant tumor cells caused their reactivation, conceivably through the TEM1-PDGFRB pathway. In vivo results have shown that absence of TEM1 does not influence primary tumor growth nor extravasation, but reduces macrometastasis formation in both intravenous and resection models of lung metastasis. These in vivo results suggest that TEM1 could be implicated in tumor mass dormancy. Computational models have shown significant correlation of TEM1 and PDGFRB as a prediction marker of low survival rates and higher relapse in various tumor types. Therefore, this study presents a pioneering research in defining the MMRN2-TEM1 reactivation axis as a potential target for state-of-the-art, personalized therapies intended for patients in remission.
Abstract (croatian)
Ponovnu pojavu tumora uzrokuju dormantne tumorske stanice koje su sposobne reaktivirati se uslijed promjena u mikrookolišnoj niši. Za endotelni protein multimerin 2 (MMRN2) se pretpostavlja da inducira nastanak tumora, stupanjem u interakciju s tumorskim endotelnim markerom 1 (TEM1). Glavni cilj ovoga rada bio je procijeniti ulogu osi MMRN2-TEM1 u reaktivaciji i proliferaciji stanica melanoma B16F10 i stanica tumora dojke E0771 te razjasniti funkciju proteina TEM1 u metastaziranju pluća. Pokazano je da os MMRN2-TEM1, inducirana kokulturom endotelnih stanica i tumorskih stanica, može stimulirati fosforilaciju proteina ERK1/2. Pojedinačni dodaci proteina PDGFRB i fragmenata MMRN2 dormantnim tumorskim stanicama uzrokovali su njihovu reaktivaciju, potencijalno signalnim putem TEM1-PDGFRB. Rezultati in vivo su pokazali da nedostatak proteina TEM1 ne utječe na rast primarnog tumora ni proces ekstravazacije, ali smanjuje formaciju makrometastaza u intravenoznom i resekcijskom modelu plućnog metastaziranja. Navedeni rezultati in vivo upućuju na to da protein TEM1 može biti uključen u regulaciju tumorske mase tijekom dormancije. Računalni modeli su ukazali na značajnu povezanost proteina TEM1 i proteina PDGFRB kao predikcijskog markera smanjenog preživljenja i češće ponovne pojave tumora kod brojnih tipova tumora. Ovaj rad predstavlja inicijalno istraživanje u definiranju reaktivacijske osi MMRN2-TEM1, kao potencijalne mete suvremenih, personaliziranih terapija namijenjenih pacijentima u remisiji.
Keywords
Keywords (croatian)
Languageenglish
URN:NBNurn:nbn:hr:217:968120
Study programmeTitle: Molecular Biology
Study programme type: university
Study level: graduate
Academic / professional title: magistar/magistra molekularne biologije (magistar/magistra molekularne biologije)
Type of resourceText
File originBorn digital
Access conditionsOpen access
Embargo expiration date: 2024-09-27
Terms of useLicence In copyright icon
Created on2022-10-21 10:13:53