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master's thesis
Istraživanje interakcija berberina i papaverina s ribonukleotidima SERS spektroskopijom
Zagreb: University of Zagreb, Faculty of Science, 2022. urn:nbn:hr:217:882475
Tikvenjak, Patricia
University of Zagreb
Faculty of Science
Department of Chemistry

Institutional repository: Repository of the Faculty of Science

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Tikvenjak, P. (2022). Istraživanje interakcija berberina i papaverina s ribonukleotidima SERS spektroskopijom (Master's thesis). Zagreb: University of Zagreb, Faculty of Science. Retrieved from https://urn.nsk.hr/urn:nbn:hr:217:882475

Tikvenjak, Patricia. "Istraživanje interakcija berberina i papaverina s ribonukleotidima SERS spektroskopijom." Master's thesis, University of Zagreb, Faculty of Science, 2022. https://urn.nsk.hr/urn:nbn:hr:217:882475

Tikvenjak, Patricia. "Istraživanje interakcija berberina i papaverina s ribonukleotidima SERS spektroskopijom." Master's thesis, University of Zagreb, Faculty of Science, 2022. https://urn.nsk.hr/urn:nbn:hr:217:882475

Tikvenjak, P. (2022). 'Istraživanje interakcija berberina i papaverina s ribonukleotidima SERS spektroskopijom', Master's thesis, University of Zagreb, Faculty of Science, accessed 23 December 2024, https://urn.nsk.hr/urn:nbn:hr:217:882475

Tikvenjak P. Istraživanje interakcija berberina i papaverina s ribonukleotidima SERS spektroskopijom [Master's thesis]. Zagreb: University of Zagreb, Faculty of Science; 2022 [cited 2024 December 23] Available at: https://urn.nsk.hr/urn:nbn:hr:217:882475

P. Tikvenjak, "Istraživanje interakcija berberina i papaverina s ribonukleotidima SERS spektroskopijom", Master's thesis, University of Zagreb, Faculty of Science, Zagreb, 2022. Available at: https://urn.nsk.hr/urn:nbn:hr:217:882475

Metadata
TitleIstraživanje interakcija berberina i papaverina s ribonukleotidima SERS spektroskopijom
Title (english)Study of berberine and papaverine interactions with ribonucleotides by SERS spectroscopy
AuthorPatricia Tikvenjak
MentorSnežana Miljanić (mentor)
Committee memberAleksandra Maršavelski (predsjednik povjerenstva)
Committee memberSnežana Miljanić (član povjerenstva)
Committee memberŽeljka Soldin (član povjerenstva)
Committee memberAdriana Kenđel (član povjerenstva)
GranterUniversity of Zagreb
Faculty of Science
(Department of Chemistry)
Zagreb
Defense date and country2022-09-30, Croatia
Scientific / art field,
discipline and subdiscipline		NATURAL SCIENCES
Chemistry
Abstract
Istraženo je površinski pojačano Ramanovo raspršenje alkaloida (berberin i papaverin), ribonukleotida (AMP, GMP, CMP, UMP) i njihovih kompleksa u koloidu srebra pripravljenom redukcijom srebrova nitrata s trinatrijevim citratom i agregiranom magnezijevim sulfatom. Na temelju koncentracijski ovisnih SERS spektara predloženi su mehanizmi adsorpcije molekula na nanočestice srebra. Alkaloidi se adsorbiraju na površinu nanočestica uslijed privlačnih elektrostatskih interakcija između pozitivnog naboja u strukturi molekula te negativnog naboja stabilizirajućeg sloja citratnih iona, dok ribonukleotidi najvjerojatnije zamjenjuju citratne ione na površini i dušikovom bazom ostvaruju vezu s površinom srebra. Spektralne promjene u SERS spektrima kompleksa pripravljenih u molarnom omjeru [alkaloid]/[ribonukleotid] = 1/1 analizirane su u odnosu na izračunati spektar zbroja SERS spektara pojedinih molekula. Opažene promjene SERS intenziteta upućuju na nastanak kompleksa i njegovu orjentaciju na površini nanočestica srebra, međutim jednoznačno ne ukazuju na vrstu interakcija između alkaloida i ribonukleotida.
Abstract (english)
Surface-enhanced Raman scattering of alkaloids (berberine and papaverine), ribonucleotides (AMP, GMP, CMP, UMP) and their complexes was studied in silver colloid prepared by reduction of silver nitrate with trisodium citrate and aggregated with magnesium sulfate. Based on concentration-dependent SERS spectra, the adsorption mechanisms of molecules on silver nanoparticles were suggested. Alkaloids were adsorbed on the nanoparticles surface due to attractive electrostatic interactions between the positive charge in the structure of the molecules and the negative charge of the stabilizing layer of citrate ions, while ribonucleotides most likely replaced citrate ions on the surface and formed a bond with the silver surface via the nitrogen base. Spectral changes in the SERS spectra of the complexes prepared in the molar ratio [alkaloid]/[ribonucleotide] = 1/1 were analyzed with respect to the calculated spectrum of the sum of the SERS spectra of individual molecules. The observed changes in SERS intensity implied the formation of a complex and its orientation on the silver nanoparticles surface, however, the type of interactions between alkaloids and ribonucleotides were not unambiguously indicated.
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:217:882475
Study programmeTitle: Graduate university studies in chemistry; specializations in: Research programme
Course: Research programme
Study programme type: university
Study level: graduate
Academic / professional title: magistar/magistra kemije (magistar/magistra kemije)
Type of resourceText
File originBorn digital
Access conditionsOpen access
Embargo expiration date: 2023-09-30
Terms of useLicence In copyright icon
Created on2022-10-24 16:29:27