prikaz prve stranice dokumenta Utjecaj hranjive podloge na specijalizirane metabolite dviju sorti vinove loze (Vitis vinifera L.) u uvjetima in vitro
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master's thesis
Utjecaj hranjive podloge na specijalizirane metabolite dviju sorti vinove loze (Vitis vinifera L.) u uvjetima in vitro
Zagreb: University of Zagreb, Faculty of Science, 2022. urn:nbn:hr:217:935990
Spudić, Iva
University of Zagreb
Faculty of Science
Department of Biology

Institutional repository: Repository of the Faculty of Science

Cite this document

Spudić, I. (2022). Utjecaj hranjive podloge na specijalizirane metabolite dviju sorti vinove loze (Vitis vinifera L.) u uvjetima in vitro (Master's thesis). Zagreb: University of Zagreb, Faculty of Science. Retrieved from https://urn.nsk.hr/urn:nbn:hr:217:935990

Spudić, Iva. "Utjecaj hranjive podloge na specijalizirane metabolite dviju sorti vinove loze (Vitis vinifera L.) u uvjetima in vitro." Master's thesis, University of Zagreb, Faculty of Science, 2022. https://urn.nsk.hr/urn:nbn:hr:217:935990

Spudić, Iva. "Utjecaj hranjive podloge na specijalizirane metabolite dviju sorti vinove loze (Vitis vinifera L.) u uvjetima in vitro." Master's thesis, University of Zagreb, Faculty of Science, 2022. https://urn.nsk.hr/urn:nbn:hr:217:935990

Spudić, I. (2022). 'Utjecaj hranjive podloge na specijalizirane metabolite dviju sorti vinove loze (Vitis vinifera L.) u uvjetima in vitro', Master's thesis, University of Zagreb, Faculty of Science, accessed 23 December 2024, https://urn.nsk.hr/urn:nbn:hr:217:935990

Spudić I. Utjecaj hranjive podloge na specijalizirane metabolite dviju sorti vinove loze (Vitis vinifera L.) u uvjetima in vitro [Master's thesis]. Zagreb: University of Zagreb, Faculty of Science; 2022 [cited 2024 December 23] Available at: https://urn.nsk.hr/urn:nbn:hr:217:935990

I. Spudić, "Utjecaj hranjive podloge na specijalizirane metabolite dviju sorti vinove loze (Vitis vinifera L.) u uvjetima in vitro", Master's thesis, University of Zagreb, Faculty of Science, Zagreb, 2022. Available at: https://urn.nsk.hr/urn:nbn:hr:217:935990

Metadata
TitleUtjecaj hranjive podloge na specijalizirane metabolite dviju sorti vinove loze (Vitis vinifera L.) u uvjetima in vitro
Title (english)Effect of nutrient medium on the specialized metabolites of two grepevines (Vitis vinifera L.) cultivars in vitro
AuthorIva Spudić
MentorDarko Preiner (mentor)
MentorZlatko Liber (komentor)
Committee memberZlatko Liber (predsjednik povjerenstva)
Committee memberVesna Petrović Peroković (član povjerenstva)
Committee memberMirela Sertić Perić (član povjerenstva)
Committee memberDraginja Mrvoš-Sermek (član povjerenstva)
GranterUniversity of Zagreb
Faculty of Science
(Department of Biology)
Zagreb
Defense date and country2022-09-26, Croatia
Scientific / art field,
discipline and subdiscipline		NATURAL SCIENCES
Biology
Abstract
Cilj ovoga rada bio je uzgojiti dvije sorte vinove loze 'Portan' i 'Graševinu' u uvjetima in vitro na dvije različite hranjive podloge, uobičajenoj podlozi koja sadrži Murashige i Skoog medij te podlozi koja osim navedenog medija sadrži i usporivač rasta, alar B9. Biljke uzgojene u uvjetima usporenoga rasta bile su manje u vidu kraćih stabljika i manjih dimenzija listova u odnosu na biljke uzgojene na uobičajenoj podlozi. Istražen je utjecaj različitih podloga na sastav i sadržaj pojedinačnih polifenolnih te hlapljivih organskih spojeva u listovima navedenih sorti. Veće vrijednosti masenih udjela gotovo svih analiziranih polifenolnih spojeva dobivene su u uzorcima listova biljaka uzgojenih na uobičajenoj podlozi, dok su gotovo svi pojedinačni hlapljivi spojevi bez obzira u koju kemijsku skupinu pripadaju (terpenoide, kiseline, aldehide, ketone i alkohole) imali veće apsolutne površine pikova u uvjetima usporenog rasta. Dobiveni rezultati potvrđuju da uporaba hranjive podloge za usporeni rast nema negativan utjecaj na biljku čime je utvrđena prikladnost njene uporabe za potrebe čuvanja genetskog materijala vinove loze u uvjetima in vitro, a čime se smanjuje učestalost subkultivacije biljaka.
Abstract (english)
The aim of this work was to grow two grape varieties 'Portan' and 'Graševina' under in vitro conditions on two different nutrient media, the usual medium containing Murashige and Skoog medium and the medium containing, in addition to the mentioned medium, a growth retarder, alar B9. Plants grown under conditions of slow growth were smaller in terms of shorter stems and smaller leaf sizes compared to plants grown on the usual substrate. The influence of different nutrient media on the composition and content of individual polyphenolic and volatile organic compounds in the leaves of the mentioned varieties was tested. Higher values of content of almost all analyzed polyphenolic compounds were obtained in leaf samples of plants grown on the usual substrate, while almost all individual volatile compounds, regardless of which chemical group they belong to (terpenoids, acids, aldehydes, ketones, and alcohols), had greater responses in the slow growth conditions. The obtained results confirm that the use of nutrient medium for slow growth does not have a negative effect on the plant, which established the suitability of its use for the purposes of preserving the genetic material of the grapevine in vitro, which reduces the frequency of subcultivation of plants.
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:217:935990
Study programmeTitle: Integrated undergraduate and graduate university study of Biology and Chemistry Education; specializations in: Integrated undergraduate and graduate university study of Biology and Chemistry Education
Course: Integrated undergraduate and graduate university study of Biology and Chemistry Education
Study programme type: university
Study level: integrated undergraduate and graduate
Academic / professional title: magistar/magistra edukacije biologije i kemije (magistar/magistra edukacije biologije i kemije)
Type of resourceText
File originBorn digital
Access conditionsOpen access
Embargo expiration date: 2024-10-01
Terms of useLicence In copyright icon
Created on2022-10-25 11:03:15