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master's thesis
Osjetljivost mutanata i nadekspresora za auksinske amidohidrolaze uročnjaka (Arabidopsis thaliana) na solni, osmotski i temperaturni stres
Zagreb: University of Zagreb, Faculty of Science, 2022. urn:nbn:hr:217:252137
Tudor, Matko
University of Zagreb
Faculty of Science
Department of Biology

Institutional repository: Repository of the Faculty of Science

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Tudor, M. (2022). Osjetljivost mutanata i nadekspresora za auksinske amidohidrolaze uročnjaka (Arabidopsis thaliana) na solni, osmotski i temperaturni stres (Master's thesis). Zagreb: University of Zagreb, Faculty of Science. Retrieved from https://urn.nsk.hr/urn:nbn:hr:217:252137

Tudor, Matko. "Osjetljivost mutanata i nadekspresora za auksinske amidohidrolaze uročnjaka (Arabidopsis thaliana) na solni, osmotski i temperaturni stres." Master's thesis, University of Zagreb, Faculty of Science, 2022. https://urn.nsk.hr/urn:nbn:hr:217:252137

Tudor, Matko. "Osjetljivost mutanata i nadekspresora za auksinske amidohidrolaze uročnjaka (Arabidopsis thaliana) na solni, osmotski i temperaturni stres." Master's thesis, University of Zagreb, Faculty of Science, 2022. https://urn.nsk.hr/urn:nbn:hr:217:252137

Tudor, M. (2022). 'Osjetljivost mutanata i nadekspresora za auksinske amidohidrolaze uročnjaka (Arabidopsis thaliana) na solni, osmotski i temperaturni stres', Master's thesis, University of Zagreb, Faculty of Science, accessed 29 November 2024, https://urn.nsk.hr/urn:nbn:hr:217:252137

Tudor M. Osjetljivost mutanata i nadekspresora za auksinske amidohidrolaze uročnjaka (Arabidopsis thaliana) na solni, osmotski i temperaturni stres [Master's thesis]. Zagreb: University of Zagreb, Faculty of Science; 2022 [cited 2024 November 29] Available at: https://urn.nsk.hr/urn:nbn:hr:217:252137

M. Tudor, "Osjetljivost mutanata i nadekspresora za auksinske amidohidrolaze uročnjaka (Arabidopsis thaliana) na solni, osmotski i temperaturni stres", Master's thesis, University of Zagreb, Faculty of Science, Zagreb, 2022. Available at: https://urn.nsk.hr/urn:nbn:hr:217:252137

Metadata
TitleOsjetljivost mutanata i nadekspresora za auksinske amidohidrolaze uročnjaka (Arabidopsis thaliana) na solni, osmotski i temperaturni stres
Title (english)The sensitivity of Arabidopsis thaliana auxin amidohydrolase mutants and overexpressors on salt, osmotic and temperature stress
AuthorMatko Tudor
MentorNataša Bauer (mentor)
Committee memberNataša Bauer (predsjednik povjerenstva)
Committee memberVišnja Besendorfer (član povjerenstva)
Committee memberIvana Šola (član povjerenstva)
Committee memberDunja Leljak-Levanić (član povjerenstva)
GranterUniversity of Zagreb
Faculty of Science
(Department of Biology)
Zagreb
Defense date and country2022-09-29, Croatia
Scientific / art field,
discipline and subdiscipline		NATURAL SCIENCES
Biology
Abstract
Za optimalni učinak, koncentracija auksina u stanicama je strogo regulirana transportom i metabolizmom auksina. Konjugati auksina predstavljaju skladišnu ili razgradnu formu auksina. Auksinske amidohidolaze su enzimi koji hidroliziraju konjugate te oslobađaju aktivni oblik auksina. U ovom istraživanju, odredio sam relativnu ekspresiju gena za auksinske amidohidrolaze ILR1, ILL2 i IAR3 u nadekspresorskim linijama ILR1 13.3, ILR1 3.1, ILL2 25.6, ILL2 27.2, ILL2 8.2, IAR3 11.5 te IAR3 13.4 vrste Arabidopsis thaliana, ekotipa Wassilewskija. Istražio sam utjecaj solnog (150 mM NaCl), osmotskog (300 mM manitola) i temperaturnog stresa (38 °C) na produžni rast i razvoj korijena kod nadekspresora i mutanata (ilr1-2, ill2-1, iar3-2, ill2-1iar3-2 i ilr1-2ill2-1iar3-2) auksinskih amidohidrolaza. Uz to, analizirao sam lokalizaciju rekombinantnog proteina ILL2-GFP u korijenu biljke nadekspresora ILL2 8.2 uzgajanih u uvjetima solnog, osmotskog i temperaturnog stresa. Nadekspresorske linije ILR1 3.1, ILR1 13.3 i ILL2 27.2 imale su 731.17, 182.5 odnosno 350.93 puta veću ekspresiju odgovarajućeg gena auksinske amidohidrolaze u odnosu na divlji tip. Navedene linije su imale i značajnu promjenu u arhitekturi korijena u uvjetima solnog stresa u odnosu na divlji tip. U uvjetima solnog i osmotskog stresa nakupljanje proteina ILL2 bilo je povećano u zoni sazrijevanja korijena, dok se u vršku korijena nije mijenjalo u odnosu na kontrolne uvjete.
Abstract (english)
Auxins act in concentration dependant manner, that is tightly controled by auxin metabolism as well as its transportation. Auxin conjugates are storage or degradable forms of auxins. Auxin amidohydrolases are enzymes that hydrolyse conjugates and release the active form of auxin. In this research, I measured relative expression of auxin amidohydrolases ILR1, ILL2 i IAR3 in Arabidopsis thaliana, ecotype Wassilewskija, overexpressing lines ILR1 13.3, ILR1 3.1, ILL2 25.6, ILL2 27.2, ILL2 8.2, IAR3 11.5 and IAR3 13.4. To reveal function of auxin amidohydrolases in plant stress response I investigated the influence of salt (150 mM NaCl), osmotic (300 mM mannitol) and temperature stress (38 °C) on growth and development of roots in overexpressing lines and auxin amidohydrolase mutants (ilr1-2, ill2-1, iar3-2, ill2- 1iar3-2 i ilr1-2ill2-1iar3-2). Further, I analysed localisation of recombinant protein ILL2-GFP in the overexpressing line ILL2 8.2, grown in conditions of salt, osmotic and temperature stress. Overexpressing lines ILR1 3.1, ILR1 13.3 and ILL2 27.2 had 731.17, 182.5 and 350.93 times higher expression of corresponding native gene compared to wild type, respectively. The root architecture of those overexpressing lines was significantly changed in salt stress condition, compared to wild type. In salt and osmotic stress, the accumulation of ILL2 was significanly increased in the root maturation zone and did not change in the root tip compared to the control conditions.
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:217:252137
Study programmeTitle: Molecular Biology
Study programme type: university
Study level: graduate
Academic / professional title: magistar/magistra molekularne biologije (magistar/magistra molekularne biologije)
Type of resourceText
File originBorn digital
Access conditionsOpen access
Terms of useLicence In copyright icon
Created on2022-10-26 10:55:50