In modern times the usage of pesticides to repel pests in food production is a necessity. Unfortunately, the pesticide residues in food can have a negative impact on consumers health. One of those widely used pesticides is metil 2-benzimidazole carbamate, also known as carbendazim, a benzimidazole fungicide whose mode of action is the inhibition of the spindle function during the cell division. This expirement was conducted in order to evaluate the genotoxic effect of carbendazim on the Swiss mouse. Groups of five male and five female mice were oraly given NOAEL dosage of carbendazim (20 mg/kg of body weight) during te period of 28 days. To evaluate the genotoxic effect micronucleus test in vivo and the single cell gel electrophoresis assay (comet assay) were used. The comet assay was conducted on blood, lymph node, spleen and thymus cells while the micronucleus test was conducted on the peripheral blood reticulocytes. The administered NOAEL dose of carbendazim during the 28 days caused high levels of DNA damage in male mice resulting in simultaneous significant increases of all three comet parameters in blood and thymus cells. Results show that there are differences of impact of carbendazim on DNA damage between sexes, that is carbendazim causes more intense DNA damage in male mice than in female mice. Carbendazim induced significant increase in the frequency of micronuclei in both sexes.