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doctoral thesis
MECHANISM OF CHROMOSOME ALIGNMENT BY MICROTUBULE OVERLAPDEPENDENT FORCES AND ITS RELEVANCE FOR CHROMOSOME SEGREGATION ERRORS
Zagreb: University of Zagreb, Faculty of Science, 2022. urn:nbn:hr:217:637095
Risteski, Patrik
University of Zagreb
Faculty of Science
Department of Biology

Institutional repository: Repository of the Faculty of Science

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Risteski, P. (2022). MECHANISM OF CHROMOSOME ALIGNMENT BY MICROTUBULE OVERLAPDEPENDENT FORCES AND ITS RELEVANCE FOR CHROMOSOME SEGREGATION ERRORS (Doctoral thesis). Zagreb: University of Zagreb, Faculty of Science. Retrieved from https://urn.nsk.hr/urn:nbn:hr:217:637095

Risteski, Patrik. "MECHANISM OF CHROMOSOME ALIGNMENT BY MICROTUBULE OVERLAPDEPENDENT FORCES AND ITS RELEVANCE FOR CHROMOSOME SEGREGATION ERRORS." Doctoral thesis, University of Zagreb, Faculty of Science, 2022. https://urn.nsk.hr/urn:nbn:hr:217:637095

Risteski, Patrik. "MECHANISM OF CHROMOSOME ALIGNMENT BY MICROTUBULE OVERLAPDEPENDENT FORCES AND ITS RELEVANCE FOR CHROMOSOME SEGREGATION ERRORS." Doctoral thesis, University of Zagreb, Faculty of Science, 2022. https://urn.nsk.hr/urn:nbn:hr:217:637095

Risteski, P. (2022). 'MECHANISM OF CHROMOSOME ALIGNMENT BY MICROTUBULE OVERLAPDEPENDENT FORCES AND ITS RELEVANCE FOR CHROMOSOME SEGREGATION ERRORS', Doctoral thesis, University of Zagreb, Faculty of Science, accessed 28 November 2024, https://urn.nsk.hr/urn:nbn:hr:217:637095

Risteski P. MECHANISM OF CHROMOSOME ALIGNMENT BY MICROTUBULE OVERLAPDEPENDENT FORCES AND ITS RELEVANCE FOR CHROMOSOME SEGREGATION ERRORS [Doctoral thesis]. Zagreb: University of Zagreb, Faculty of Science; 2022 [cited 2024 November 28] Available at: https://urn.nsk.hr/urn:nbn:hr:217:637095

P. Risteski, "MECHANISM OF CHROMOSOME ALIGNMENT BY MICROTUBULE OVERLAPDEPENDENT FORCES AND ITS RELEVANCE FOR CHROMOSOME SEGREGATION ERRORS", Doctoral thesis, University of Zagreb, Faculty of Science, Zagreb, 2022. Available at: https://urn.nsk.hr/urn:nbn:hr:217:637095

Metadata
TitleMECHANISM OF CHROMOSOME ALIGNMENT BY MICROTUBULE OVERLAPDEPENDENT FORCES AND ITS RELEVANCE FOR CHROMOSOME SEGREGATION ERRORS
Title (croatian)MEHANIZAM PORAVNANJA KROMOSOMA SILAMA KOJE OVISE O PREKLAPANJU MIKROTUBULA I NJEGOV UTJECAJ NA POGREŠKE U RAZDVAJANJU KROMOSOMA
AuthorPatrik Risteski
MentorIva Marija Tolić (mentor)
Committee memberVedrana Filić Mileta (predsjednik povjerenstva)
Committee memberBiljana Balen (član povjerenstva)
GranterUniversity of Zagreb
Faculty of Science
(Department of Biology)
Zagreb
Defense date and country2022-10-11, Croatia
Scientific / art field,
discipline and subdiscipline		NATURAL SCIENCES
Biology
Universal decimal classification
(UDC)		576 - Cellular and subcellular biology. Cytology
Abstract
Chromosome alignment is a hallmark of mitosis and alignment defects, which persist in anaphase, lead to direct aneuploidy. Alignment at the spindle equator depends on pulling forces exerted at kinetochore fiber tips together with polar ejection forces. However, kinetochore fibers are also subjected to forces that drive their poleward flux. Here I introduce a microtubule flux-driven centering mechanism that relies on flux generated by forces within the overlaps of bridging and kinetochore fibers. This centering mechanism works so that the longer kinetochore fiber fluxes faster than the shorter one, moving the kinetochores towards the center. Kinetochores are better centered when overlaps are shorter and the kinetochore fiber flux is markedly slower than the bridging fiber flux. I identified Kif18A and Kif4A proteins as overlap and flux regulators and NuMA protein as a fiber coupler. Thus, I propose that length-dependent sliding forces exerted by the bridging fiber onto kinetochore fibers support chromosome alignment.
Abstract (croatian)
Poravnanje kromosoma je jedno od obilježja mitoze i problemi u poravnavanju, koji opstaju u anafazi, vode izravnoj aneuploidiji. Poravnanje na ekvatoru vretena ovisi o silama vučenja koje djeluju na vrhove kinetohornih vlakana zajedno sa silama izbacivanja s pola. Međutim, kinetohorna vlakna također su podvrgnuta silama koje pokreću njihov tok prema polu. U ovom radu predstavljam mehanizam za centriranje vođen tokom mikrotubula koji se oslanja na tok generiran silama unutar preklopa između premošćujućih i kinetohornih vlakana. Ovaj mehanizam centriranja radi tako da duže kinetohorno vlakno teče brže od kraćeg, što pomiče kinetohore prema centru. Kinetohore su bolje centrirane kada su preklapanja kraća i tok kinetohornih vlakana izrazito sporiji od toka premošćujućih vlakana. Identificirao sam proteine Kif18A i Kif4A kao regulatore preklapanja i toka mikrotubula i protein NuMA kao spojnicu vlakana. Stoga predlažem da sile klizanja koje su ovisne o duljini, a koje premošćujuće vlakno prenosi na kinetohorna vlakna, podržavaju poravnanje kromosoma.
Keywords
Keywords (croatian)
Languageenglish
URN:NBNurn:nbn:hr:217:637095
Promotion2022
Study programmeTitle: Biology
Study programme type: university
Study level: postgraduate
Academic / professional title: doktor/doktorica znanosti, područje prirodnih znanosti, polje biologija (doktor/doktorica znanosti, područje prirodnih znanosti, polje biologija)
Type of resourceText
File originBorn digital
Access conditionsOpen access
Terms of useLicence In copyright icon
Created on2022-11-17 11:51:28