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doctoral thesis
Usporedba toksičnoga djelovanja nanočestica i ionskoga srebra na vodenu leću (Lemna minor L.)
Zagreb: University of Zagreb, Faculty of Science, 2022. urn:nbn:hr:217:396331
Glavaš Ljubimir, Katarina
University of Zagreb
Faculty of Science
Department of Biology

Institutional repository: Repository of the Faculty of Science

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Glavaš Ljubimir, K. (2022). Usporedba toksičnoga djelovanja nanočestica i ionskoga srebra na vodenu leću (Lemna minor L.) (Doctoral thesis). Zagreb: University of Zagreb, Faculty of Science. Retrieved from https://urn.nsk.hr/urn:nbn:hr:217:396331

Glavaš Ljubimir, Katarina. "Usporedba toksičnoga djelovanja nanočestica i ionskoga srebra na vodenu leću (Lemna minor L.)." Doctoral thesis, University of Zagreb, Faculty of Science, 2022. https://urn.nsk.hr/urn:nbn:hr:217:396331

Glavaš Ljubimir, Katarina. "Usporedba toksičnoga djelovanja nanočestica i ionskoga srebra na vodenu leću (Lemna minor L.)." Doctoral thesis, University of Zagreb, Faculty of Science, 2022. https://urn.nsk.hr/urn:nbn:hr:217:396331

Glavaš Ljubimir, K. (2022). 'Usporedba toksičnoga djelovanja nanočestica i ionskoga srebra na vodenu leću (Lemna minor L.)', Doctoral thesis, University of Zagreb, Faculty of Science, accessed 23 December 2024, https://urn.nsk.hr/urn:nbn:hr:217:396331

Glavaš Ljubimir K. Usporedba toksičnoga djelovanja nanočestica i ionskoga srebra na vodenu leću (Lemna minor L.) [Doctoral thesis]. Zagreb: University of Zagreb, Faculty of Science; 2022 [cited 2024 December 23] Available at: https://urn.nsk.hr/urn:nbn:hr:217:396331

K. Glavaš Ljubimir, "Usporedba toksičnoga djelovanja nanočestica i ionskoga srebra na vodenu leću (Lemna minor L.)", Doctoral thesis, University of Zagreb, Faculty of Science, Zagreb, 2022. Available at: https://urn.nsk.hr/urn:nbn:hr:217:396331

Metadata
TitleUsporedba toksičnoga djelovanja nanočestica i ionskoga srebra na vodenu leću (Lemna minor L.)
Title (english)Comparison of nanosilver and ionic silver toxicity in duckweed (Lemna minor L.)
AuthorKatarina Glavaš Ljubimir
MentorSandra Radić Brkanac (mentor)
Committee memberBiljana Balen (predsjednik povjerenstva)
Committee memberNada Oršolić (član povjerenstva)
GranterUniversity of Zagreb
Faculty of Science
(Department of Biology)
Zagreb
Defense date and country2022-02-04, Croatia
Scientific / art field,
discipline and subdiscipline		NATURAL SCIENCES
Biology
Universal decimal classification
(UDC)		57 - Biological sciences. Physical anthropology. Biodiversity
Abstract
Kontinuirana proizvodnja i upotreba nanočestica rezultira njihovim značajnim otpuštanjem u okoliš. Zahvaljujući svojim antibakterijskim svojstvima, nanočestice srebra (nAg) spadaju u najčešće korištene nanočestice u proizvodima široke potrošnje. Otpuštanjem nAg u vodeni okoliš, može doći do njihove akumulacije u vodenim biljkama i daljnjeg prijenosa kroz hranidbeni lanac. Cilj ovog istraživanja bio je procijeniti i usporediti toksičnost nAg i AgNO3 (ionsko srebro; iAg) na vodenu leću Lemna minor u rasponu koncentracija 0,5-25 mg/L. U tu svrhu nAg su karakterizirane, mjereno je nakupljanje Ag u biljci, stopa rasta, fluorescencija klorofila a, sadržaj fotosintetskih pigmenata te niz pokazatelja oksidacijskog stresa. Akumulacija Ag bila je višestruko veća u vodenoj leći izloženoj iAg nego u onoj izloženoj nAg. Smanjenje stope rasta, funkcionalnosti fotosustava II i sadržaja fotosintetskih pigmenata zabilježeno je u odgovoru na oba oblika Ag, no posebno pod utjecajem iAg. Sadržaj reaktivnih oblika kisika bio je povećan pod utjecajem oba oblika Ag, no iAg je uzrokovao znatnu veću razinu lipidne peroksidacije u vodenoj leći nego nAg. Aktivnost mjerenih antioksidacijskih enzima uglavnom je bila povećana pod utjecajem nAg te inhibirana pod utjecajem iAg, dok je sadržaj glutationa bio podjednako smanjen u odgovoru na oba oblika Ag. Zaključno, utvrđeno je da je iAg toksičnije za vodenu leću nego nAg. Takvi se rezultati mogu povezati s većim nakupljanjem Ag u vodenoj leći izloženoj iAg u odnosu na kontrolu, i posljedično jačim oksidacijskim stresom, koje je dovelo do smanjenja antioksidacijskih obrambenih mehanizama.
Abstract (english)
Continuous formation and utilization of nanoparticles have resulted with their significant discharge into the environment. Due to their antibacterial properties, silver nanoparticles (nAg) are increasingly used in consumer products. When released into the aquatic environment, nAg can be taken up by aquatic plants and transferred along the food chain. The aim of this work was to evaluate and compare toxicity of AgNO3 (ionic silver; iAg) and nanosilver in the concentration range 0,5-25 mg/L using duckweed Lemna minor as a model plant. For that purpose, characterization of nAg was done, and Ag uptake by plants, growth rate, chlorophyll a fluorescence, photosynthetic pigments content as well as a number of oxidative stress parameters were determined. Accumulation of Ag was several times higher in plants treated with iAg compared to nAg. Both forms of Ag significantly reduced growth rate, photosystem II functionality and photosynthetic pigments content, but the effect of iAg was more pronounced. Reactive oxygen species content increased under both forms of Ag, though iAg caused much greater extent of lipid peroxidation than nAg. Induction of antioxidative enzyme activities was mosty observed in response to nAg, while iAg caused their inhibition. The content of glutathione decreased to the same degree under both iAg and nAg. The results indicate that ionic silver is more toxic to duckweed than nanosilver. Such findings can be correlated with higer Ag accumulation in duckweed exposed to iAg, and consequently greater extent of oxidative stress, leading to impaired antioxidative defense mechanisms.
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:217:396331
Promotion2022
Study programmeTitle: Biology
Study programme type: university
Study level: postgraduate
Academic / professional title: doktor/doktorica znanosti, područje prirodnih znanosti, polje biologija (doktor/doktorica znanosti, područje prirodnih znanosti, polje biologija)
Type of resourceText
File originBorn digital
Access conditionsOpen access
Terms of useLicence In copyright icon
Created on2022-11-22 12:00:03