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doctoral thesis
HUMAN NME6 PROTEIN: SUBCELLULAR LOCALIZATION, STRUCTURE AND FUNCTION
Zagreb: University of Zagreb, Faculty of Science, 2022. urn:nbn:hr:217:788932
Proust, Bastien Lucien Jean
University of Zagreb
Faculty of Science
Department of Biology

Institutional repository: Repository of the Faculty of Science

Cite this document

Proust, B. L. J. (2022). HUMAN NME6 PROTEIN: SUBCELLULAR LOCALIZATION, STRUCTURE AND FUNCTION (Doctoral thesis). Zagreb: University of Zagreb, Faculty of Science. Retrieved from https://urn.nsk.hr/urn:nbn:hr:217:788932

Proust, Bastien Lucien Jean. "HUMAN NME6 PROTEIN: SUBCELLULAR LOCALIZATION, STRUCTURE AND FUNCTION." Doctoral thesis, University of Zagreb, Faculty of Science, 2022. https://urn.nsk.hr/urn:nbn:hr:217:788932

Proust, Bastien Lucien Jean. "HUMAN NME6 PROTEIN: SUBCELLULAR LOCALIZATION, STRUCTURE AND FUNCTION." Doctoral thesis, University of Zagreb, Faculty of Science, 2022. https://urn.nsk.hr/urn:nbn:hr:217:788932

Proust, B. L. J. (2022). 'HUMAN NME6 PROTEIN: SUBCELLULAR LOCALIZATION, STRUCTURE AND FUNCTION', Doctoral thesis, University of Zagreb, Faculty of Science, accessed 13 October 2024, https://urn.nsk.hr/urn:nbn:hr:217:788932

Proust BLJ. HUMAN NME6 PROTEIN: SUBCELLULAR LOCALIZATION, STRUCTURE AND FUNCTION [Doctoral thesis]. Zagreb: University of Zagreb, Faculty of Science; 2022 [cited 2024 October 13] Available at: https://urn.nsk.hr/urn:nbn:hr:217:788932

B. L. J. Proust, "HUMAN NME6 PROTEIN: SUBCELLULAR LOCALIZATION, STRUCTURE AND FUNCTION", Doctoral thesis, University of Zagreb, Faculty of Science, Zagreb, 2022. Available at: https://urn.nsk.hr/urn:nbn:hr:217:788932

Metadata
TitleHUMAN NME6 PROTEIN: SUBCELLULAR LOCALIZATION, STRUCTURE AND FUNCTION
Title (croatian)UNUTARSTANIČNI SMJEŠTAJ, STRUKTURA I FUNKCIJA PROTEINA NME6 U ČOVJEKA
AuthorBastien Lucien Jean Proust
MentorMaja Herak-Bosnar (mentor)
Committee memberMaja Matulić (predsjednik povjerenstva)
Committee memberBiljana Balen (član povjerenstva)
GranterUniversity of Zagreb
Faculty of Science
(Department of Biology)
Zagreb
Defense date and country2022-11-25, Croatia
Scientific / art field,
discipline and subdiscipline		NATURAL SCIENCES
Biology
Universal decimal classification
(UDC)		577 - Biochemistry. Molecular biology. Biophysics
Abstract
Nucleoside diphosphate kinase (NDPK/NME/NM23) family of enzymes catalyze the transfer of gamma phosphate group from nucleoside triphosphates to nucleoside diphosphates. The family sparked interest after the discovery of metastasis suppressor activity of NME1, leading to a comprehensive characterization of Group I members (NME1-NME4). In contrast, the evolutionary ancient Group II members (NME5-NME9) are to date barely explored. We aimed to produce an extensive description of the human NME6 protein, by revealing its expression pattern, enzymatic activity, subcellular localization, quaternary structure and cellular function. Our results show the ubiquitous expression of NME6. We demonstrated a lack of enzymatic activity linked to oligomerization deficiency. We confirmed the mitochondrial localization and refined it to a matrix-facing protein, partially bound to the mitochondrial inner membrane, where NME6 interacts with RCC1L protein. Finally, we unraveled a negative impact of NME6 on mitochondrial respiration, possibly linked to the regulation of mitochondria-encoded protein expression.
Abstract (croatian)
Nukleozid difosfatske kinaze, (NDPK/NME/NM23), kataliziraju prijenos gama fosfata s nukleozid trifosfata na nukleozid difosfate. Ova obitelj enzima privukla je veliku pozornost nakon otkrića da je jedan od njenih članova, NME1, uključen u sprječavanje nastanka metastaza. To je dovelo do sveobuhvatnih istraživanja članova NME iz skupine I (od NME1 do NME4). Nasuprot tome, evolucijski „stariji“ članovi Grupe II (od NME5 do NME9) do danas su vrlo malo istraženi. Cilj ovog rada bio je detaljnije istražiti ljudski protein NME6, utvrditi njegovu ekspresiju, enzimsku aktivnosti, unutarstanični smještaj, kvarternu strukturu i stanične funkcije. Naši rezultati pokazuju sveprisutnu ekspresiju proteina NME6. Pokazali smo da NME6 ne stvara oligomere te da, u vezi s time, ne pokazuje ni enzimatsku aktivnost. Pokazali smo da je protein smješten u matriksu mitohondrija, vjerojatno djelomično vezan na unutarnju membranu, gdje stupa u interakciju s proteinom RCC1L. Konačno, otkrili smo negativan utjecaj pojačane ekspresije NME6 na stanično disanje, što je vjerojatno povezano s regulacijom ekspresije proteina kodiranih mitohondrijskom DNA.
Keywords
Keywords (croatian)
Languageenglish
URN:NBNurn:nbn:hr:217:788932
Promotion2022
Study programmeTitle: Biology
Study programme type: university
Study level: postgraduate
Academic / professional title: doktor/doktorica znanosti, područje prirodnih znanosti, polje biologija (doktor/doktorica znanosti, područje prirodnih znanosti, polje biologija)
Type of resourceText
File originBorn digital
Access conditionsOpen access
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Created on2022-12-12 11:30:11