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master's thesis
Procjena genotoksičnog učinka pesticida imazalila primjenom alkalnog komet-testa i mikronukleus-testa u miša
Zagreb: University of Zagreb, Faculty of Science, 2011. urn:nbn:hr:217:889771
Šibić, Leana
University of Zagreb
Faculty of Science
Department of Biology

Institutional repository: Repository of the Faculty of Science

Cite this document

Šibić, L. (2011). Procjena genotoksičnog učinka pesticida imazalila primjenom alkalnog komet-testa i mikronukleus-testa u miša (Master's thesis). Zagreb: University of Zagreb, Faculty of Science. Retrieved from https://urn.nsk.hr/urn:nbn:hr:217:889771

Šibić, Leana. "Procjena genotoksičnog učinka pesticida imazalila primjenom alkalnog komet-testa i mikronukleus-testa u miša." Master's thesis, University of Zagreb, Faculty of Science, 2011. https://urn.nsk.hr/urn:nbn:hr:217:889771

Šibić, Leana. "Procjena genotoksičnog učinka pesticida imazalila primjenom alkalnog komet-testa i mikronukleus-testa u miša." Master's thesis, University of Zagreb, Faculty of Science, 2011. https://urn.nsk.hr/urn:nbn:hr:217:889771

Šibić, L. (2011). 'Procjena genotoksičnog učinka pesticida imazalila primjenom alkalnog komet-testa i mikronukleus-testa u miša', Master's thesis, University of Zagreb, Faculty of Science, accessed 27 December 2024, https://urn.nsk.hr/urn:nbn:hr:217:889771

Šibić L. Procjena genotoksičnog učinka pesticida imazalila primjenom alkalnog komet-testa i mikronukleus-testa u miša [Master's thesis]. Zagreb: University of Zagreb, Faculty of Science; 2011 [cited 2024 December 27] Available at: https://urn.nsk.hr/urn:nbn:hr:217:889771

L. Šibić, "Procjena genotoksičnog učinka pesticida imazalila primjenom alkalnog komet-testa i mikronukleus-testa u miša", Master's thesis, University of Zagreb, Faculty of Science, Zagreb, 2011. Available at: https://urn.nsk.hr/urn:nbn:hr:217:889771

Metadata
TitleProcjena genotoksičnog učinka pesticida imazalila primjenom alkalnog komet-testa i mikronukleus-testa u miša
Title (english)Evaluation of genotoxic effect of pesticide Imazalil using alkaline comet assay and micronucelus assay in mice
AuthorLeana Šibić
MentorVesna Benković (mentor)
Committee memberVesna Benković (predsjednik povjerenstva)
Committee memberAlan Moro (član povjerenstva)
Committee memberDanijel Orešić (član povjerenstva)
Committee memberMaria Špoljar (član povjerenstva)
GranterUniversity of Zagreb
Faculty of Science
(Department of Biology)
Zagreb
Defense date and country2011-07-08, Croatia
Scientific / art field,
discipline and subdiscipline		NATURAL SCIENCES
Biology
Abstract
Imazalil je fungicid, koji je široko rasprostranjen u poljoprivredi, koristi se za suzbijanje gljivica na voću, povrću i ukrasnom bilju. Također se koristi u tretmanima sjemenja nakon žetve te u sprečavanju propadanja citrusnog voća i banana prilikom skladištenja. Cilj ovog istraživanja bio je utvrditi genotoksični učinak ovog pesticida na tkiva pojedinih organa (slezena, limfni čvorovi, timus i krv) Swiss miševa. Plan pokusa bio je sukladan odredbama OECD 407. Životinje su raspoređene u 2 skupine (kontrolna i tretirana, u svakoj skupini je po 5 muških i 5 ženskih životinja). Eksperimentalne životinje tretirane su NOAEL dozom pesticida Imazalila (10 mg/kg) koji je svakodnevno primjenjivan oralno tijekom 28 dana, nakon čega je izvršen alkalni komet test na stanicama krvi, limfnih čvorova, slezene i timusa i brojanje mikronukleusa u perifernoj krvi. Imazalil je inducirao značajno povećanje učestalosti mikronukleusa kod oba spola. Uočili smo povećanje vrijednosti duljine repa kometa u tretiranoj skupini životinja na stanice četiri ispitivana organa u odnosu na kontrolnu skupinu. Značajno povećanje vrijednosti duljine repa u stanicama krvi, slezene, timusa i limfnih čvorova ukazuje na jači genotoksični učinak Imazalila u ženskih nego u muških miševa.
Abstract (english)
Imazalil is a fungicide that is widely used in agriculture to control fungi on fruits, vegetables and ornamentals. It is also used in treatment of seeds after harvest and to prevent deterioration of citrus fruits and bananas during storage. The aim of this research was to determine the genotoxic effects of this pesticide in tissues of some organs (spleen, lymph nodes, thymus and blood) in Swiss mice. The plan of experiment was consistent with written standard OECD 407. The animals were classified in two groups (control and treated, in each group by 5 male and 5 female animals). Experimental animals were exposed to the NOAEL dose of the pesticide Imazalil (10 mg / kg) which was daily administered orally for 28 days, after which we made alkaline comet assay performed on cells of the blood, lymph nodes, spleen and thymus, and counting of micronuclei in peripheral blood. Imazalil induced significant increase in the frequency of micronuclei in both sexes. We found increased values of comet tail length in the treated group of animals in cells of four examined organs in relation to the control group. The significant increase in the value of tail length in blood cells, spleen, thymus and lymph nodes indicates a stronger genotoxic effect of Imazalil in the female than in male mice.
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:217:889771
Study programmeTitle: Enviromental Sciences
Study programme type: university
Study level: graduate
Academic / professional title: magistar/magistra struke znanosti o okolišu (magistar/magistra struke znanosti o okolišu)
Type of resourceText
File originBorn digital
Access conditionsAccess restricted to students and staff of home institution
Terms of useLicence In copyright icon
Created on2017-05-03 10:25:13